HIC-5: A Mobile Molecular Scaffold Regulating the Anchorage Dependence of Cell Growth

HIC-5 is a multidomain LIM protein homologous to paxillin that serves as a molecular scaffold at focal adhesions and in the nucleus. It forms mobile molecular units with LIM-only proteins, PINCH, and CRP2 and translocates in and out of the nucleus via a nuclear export signal (NES). Of note, NES of H...

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Main Authors: Motoko Shibanuma, Kazunori Mori, Kiyoshi Nose
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:International Journal of Cell Biology
Online Access:http://dx.doi.org/10.1155/2012/426138
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author Motoko Shibanuma
Kazunori Mori
Kiyoshi Nose
author_facet Motoko Shibanuma
Kazunori Mori
Kiyoshi Nose
author_sort Motoko Shibanuma
collection DOAJ
description HIC-5 is a multidomain LIM protein homologous to paxillin that serves as a molecular scaffold at focal adhesions and in the nucleus. It forms mobile molecular units with LIM-only proteins, PINCH, and CRP2 and translocates in and out of the nucleus via a nuclear export signal (NES). Of note, NES of HIC-5 is distinctive in its sensitivity to the cellular redox state. Recently, the mobile units of HIC-5 have been suggested to be involved in the regulation of the anchorage dependence of cell growth. On loss of adhesion, an increase in reactive oxygen species in the cells modifies NES and stops shuttling, which leads to cell-cycle control. More specifically, the system circumvents nuclear localization of cyclin D1 and transactivates p21Cip1 in detached cells, thereby avoiding anchorage-independent cell growth. Thus, the HIC-5-LIM only protein complex has emerged as a fail-safe system for regulating the anchorage dependence of cell growth.
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series International Journal of Cell Biology
spelling doaj-art-6023ca48c1164cb097d033a3bffa8c192025-08-20T02:35:21ZengWileyInternational Journal of Cell Biology1687-88761687-88842012-01-01201210.1155/2012/426138426138HIC-5: A Mobile Molecular Scaffold Regulating the Anchorage Dependence of Cell GrowthMotoko Shibanuma0Kazunori Mori1Kiyoshi Nose2Department of Cancer Cell Biology, Showa University School of Pharmacy, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Cancer Cell Biology, Showa University School of Pharmacy, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Cancer Cell Biology, Showa University School of Pharmacy, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanHIC-5 is a multidomain LIM protein homologous to paxillin that serves as a molecular scaffold at focal adhesions and in the nucleus. It forms mobile molecular units with LIM-only proteins, PINCH, and CRP2 and translocates in and out of the nucleus via a nuclear export signal (NES). Of note, NES of HIC-5 is distinctive in its sensitivity to the cellular redox state. Recently, the mobile units of HIC-5 have been suggested to be involved in the regulation of the anchorage dependence of cell growth. On loss of adhesion, an increase in reactive oxygen species in the cells modifies NES and stops shuttling, which leads to cell-cycle control. More specifically, the system circumvents nuclear localization of cyclin D1 and transactivates p21Cip1 in detached cells, thereby avoiding anchorage-independent cell growth. Thus, the HIC-5-LIM only protein complex has emerged as a fail-safe system for regulating the anchorage dependence of cell growth.http://dx.doi.org/10.1155/2012/426138
spellingShingle Motoko Shibanuma
Kazunori Mori
Kiyoshi Nose
HIC-5: A Mobile Molecular Scaffold Regulating the Anchorage Dependence of Cell Growth
International Journal of Cell Biology
title HIC-5: A Mobile Molecular Scaffold Regulating the Anchorage Dependence of Cell Growth
title_full HIC-5: A Mobile Molecular Scaffold Regulating the Anchorage Dependence of Cell Growth
title_fullStr HIC-5: A Mobile Molecular Scaffold Regulating the Anchorage Dependence of Cell Growth
title_full_unstemmed HIC-5: A Mobile Molecular Scaffold Regulating the Anchorage Dependence of Cell Growth
title_short HIC-5: A Mobile Molecular Scaffold Regulating the Anchorage Dependence of Cell Growth
title_sort hic 5 a mobile molecular scaffold regulating the anchorage dependence of cell growth
url http://dx.doi.org/10.1155/2012/426138
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AT kiyoshinose hic5amobilemolecularscaffoldregulatingtheanchoragedependenceofcellgrowth