Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Primary Canine Mast Cell Tumors and Related Regional Lymph Node Metastasis
Vascular endothelial growth factor (VEGF) is an important regulator of angiogenesis and has been identified as an autocrine growth factor for neoplastic cells of several tumors. The aim of this study was to evaluate VEGF expression in canine mast cell tumors and their respective lymph node metastase...
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MDPI AG
2025-01-01
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| author | Alice Corrêa Rassele Isabella Oliveira Almeida Maylla Garschagen Gava Pedro Antônio Bronhara Pimentel Antonio Giuliano Felipe Augusto Ruiz Sueiro Ayisa Rodrigues de Oliveira Andrigo Barboza de Nardi Rodrigo dos Santos Horta |
| author_facet | Alice Corrêa Rassele Isabella Oliveira Almeida Maylla Garschagen Gava Pedro Antônio Bronhara Pimentel Antonio Giuliano Felipe Augusto Ruiz Sueiro Ayisa Rodrigues de Oliveira Andrigo Barboza de Nardi Rodrigo dos Santos Horta |
| author_sort | Alice Corrêa Rassele |
| collection | DOAJ |
| description | Vascular endothelial growth factor (VEGF) is an important regulator of angiogenesis and has been identified as an autocrine growth factor for neoplastic cells of several tumors. The aim of this study was to evaluate VEGF expression in canine mast cell tumors and their respective lymph node metastases. For this purpose, 28 patients with mast cell tumors and confirmed regional lymph node metastasis were selected. The samples were submitted for analysis with immunohistochemistry. VEGF was expressed in 14/28 patients (50%) and 35.7% of primary MCTs (10/28), and only 14.3% expressed VEGF in both the primary tumor and its respective metastasis (4/28), with fair agreement (Κ = 0.250). There was a weak correlation between VEGF and tumor size (<i>p</i> = 0.016, rs = 0.045). In this cohort, survival time was correlated with Kiupel grade, mitotic index, tumor necrosis, tumor location, and systemic treatment. VEGF immunolabeling had no influence on survival; however, patients with positive expression may benefit from specific therapy. |
| format | Article |
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| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-6015a6768cf74bbca8f64e6d3076beeb2025-01-24T13:18:26ZengMDPI AGAnimals2076-26152025-01-0115228310.3390/ani15020283Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Primary Canine Mast Cell Tumors and Related Regional Lymph Node MetastasisAlice Corrêa Rassele0Isabella Oliveira Almeida1Maylla Garschagen Gava2Pedro Antônio Bronhara Pimentel3Antonio Giuliano4Felipe Augusto Ruiz Sueiro5Ayisa Rodrigues de Oliveira6Andrigo Barboza de Nardi7Rodrigo dos Santos Horta8Department of Veterinary Medicine and Surgery, Agricultural and Veterinary Sciences, Universidade Estadual Paulista, Jaboticabal 14884-900, BrazilDepartment of Veterinary Medicine and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte 31310-250, BrazilMaylla Gava Patologia Veterinaria, Praia das Gaivotas, Vila Velha 29102-571, BrazilDepartment of Veterinary Medicine and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte 31310-250, BrazilDepartment of Veterinary Clinical Science, Jockey Club College of Veterinary Medicine, City University of Hong Kong, Kowloon, Hong KongVETPAT, Campinas 13070-070, BrazilDepartment of Veterinary Medicine and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte 31310-250, BrazilDepartment of Veterinary Medicine and Surgery, Agricultural and Veterinary Sciences, Universidade Estadual Paulista, Jaboticabal 14884-900, BrazilDepartment of Veterinary Medicine and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte 31310-250, BrazilVascular endothelial growth factor (VEGF) is an important regulator of angiogenesis and has been identified as an autocrine growth factor for neoplastic cells of several tumors. The aim of this study was to evaluate VEGF expression in canine mast cell tumors and their respective lymph node metastases. For this purpose, 28 patients with mast cell tumors and confirmed regional lymph node metastasis were selected. The samples were submitted for analysis with immunohistochemistry. VEGF was expressed in 14/28 patients (50%) and 35.7% of primary MCTs (10/28), and only 14.3% expressed VEGF in both the primary tumor and its respective metastasis (4/28), with fair agreement (Κ = 0.250). There was a weak correlation between VEGF and tumor size (<i>p</i> = 0.016, rs = 0.045). In this cohort, survival time was correlated with Kiupel grade, mitotic index, tumor necrosis, tumor location, and systemic treatment. VEGF immunolabeling had no influence on survival; however, patients with positive expression may benefit from specific therapy.https://www.mdpi.com/2076-2615/15/2/283neoplasmdogprognostictreatmentdissemination |
| spellingShingle | Alice Corrêa Rassele Isabella Oliveira Almeida Maylla Garschagen Gava Pedro Antônio Bronhara Pimentel Antonio Giuliano Felipe Augusto Ruiz Sueiro Ayisa Rodrigues de Oliveira Andrigo Barboza de Nardi Rodrigo dos Santos Horta Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Primary Canine Mast Cell Tumors and Related Regional Lymph Node Metastasis Animals neoplasm dog prognostic treatment dissemination |
| title | Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Primary Canine Mast Cell Tumors and Related Regional Lymph Node Metastasis |
| title_full | Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Primary Canine Mast Cell Tumors and Related Regional Lymph Node Metastasis |
| title_fullStr | Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Primary Canine Mast Cell Tumors and Related Regional Lymph Node Metastasis |
| title_full_unstemmed | Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Primary Canine Mast Cell Tumors and Related Regional Lymph Node Metastasis |
| title_short | Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Primary Canine Mast Cell Tumors and Related Regional Lymph Node Metastasis |
| title_sort | immunohistochemical expression of vascular endothelial growth factor vegf in primary canine mast cell tumors and related regional lymph node metastasis |
| topic | neoplasm dog prognostic treatment dissemination |
| url | https://www.mdpi.com/2076-2615/15/2/283 |
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