Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products
In this study, a label-free, portable and reproducible immunochip based on quartz crystal microbalance (QCM) was developed for the qualitative detection of β-lactoglobulin (β-LG), an allergen, in milk products. Experimental parameters in the fabrication and regeneration procedure such as pH of the c...
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Tsinghua University Press
2022-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000532 |
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| author | Mingfei Pan Liping Hong Jingying Yang Xiaoqian Xie Kaixin Liu Shuo Wang |
| author_facet | Mingfei Pan Liping Hong Jingying Yang Xiaoqian Xie Kaixin Liu Shuo Wang |
| author_sort | Mingfei Pan |
| collection | DOAJ |
| description | In this study, a label-free, portable and reproducible immunochip based on quartz crystal microbalance (QCM) was developed for the qualitative detection of β-lactoglobulin (β-LG), an allergen, in milk products. Experimental parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment, amount of anti-β-LG antibody and regeneration reagent were optimized in detail. Under optimal conditions, the proposed QCM immunochip exhibited good recognition of β-LG, with a calibration curve of ΔF = 12.877Cβ-LG0.4809 (R2 = 0.9982) and limit of detection of 0.04 μg/mL. Additionally, this portable QCM immunochip had good stability, high specificity, and no obvious cross-reaction to three other milk proteins (α-casein, α-lactalbumin, and lactoferrin). It could compete a qualitative measurement within 5 min, and could be reused at least ten times. In the β-LG analysis of actual milk samples, the developed QCM immunochip yielded reliable and accurate results, which correlated strongly with those from the standard HPLC method (R2 = 0.9969). Thus, the portable, stable, and reproducible QCM immunochip developed in this study allowed the rapid, cost-effectively and sensitively measure the β-LG in milk products. |
| format | Article |
| id | doaj-art-6006451f03094609adb8654d2afd3293 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-6006451f03094609adb8654d2afd32932025-02-03T06:55:41ZengTsinghua University PressFood Science and Human Wellness2213-45302022-09-0111513151321Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk productsMingfei Pan0Liping Hong1Jingying Yang2Xiaoqian Xie3Kaixin Liu4Shuo Wang5Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China; Corresponding author at: Tianjin University of Science & Technology, Tianjin 300457, China. Fax: (86 22) 60912493.In this study, a label-free, portable and reproducible immunochip based on quartz crystal microbalance (QCM) was developed for the qualitative detection of β-lactoglobulin (β-LG), an allergen, in milk products. Experimental parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment, amount of anti-β-LG antibody and regeneration reagent were optimized in detail. Under optimal conditions, the proposed QCM immunochip exhibited good recognition of β-LG, with a calibration curve of ΔF = 12.877Cβ-LG0.4809 (R2 = 0.9982) and limit of detection of 0.04 μg/mL. Additionally, this portable QCM immunochip had good stability, high specificity, and no obvious cross-reaction to three other milk proteins (α-casein, α-lactalbumin, and lactoferrin). It could compete a qualitative measurement within 5 min, and could be reused at least ten times. In the β-LG analysis of actual milk samples, the developed QCM immunochip yielded reliable and accurate results, which correlated strongly with those from the standard HPLC method (R2 = 0.9969). Thus, the portable, stable, and reproducible QCM immunochip developed in this study allowed the rapid, cost-effectively and sensitively measure the β-LG in milk products.http://www.sciencedirect.com/science/article/pii/S2213453022000532β-Lactoglobulin allergenQuartz crystal microbalanceImmunochipMilk products |
| spellingShingle | Mingfei Pan Liping Hong Jingying Yang Xiaoqian Xie Kaixin Liu Shuo Wang Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products Food Science and Human Wellness β-Lactoglobulin allergen Quartz crystal microbalance Immunochip Milk products |
| title | Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products |
| title_full | Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products |
| title_fullStr | Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products |
| title_full_unstemmed | Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products |
| title_short | Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products |
| title_sort | fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label free detection of β lactoglobulin allergen in milk products |
| topic | β-Lactoglobulin allergen Quartz crystal microbalance Immunochip Milk products |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000532 |
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