Purification and identification of anti-inflammatory peptides from sturgeon (Acipenser schrenckii) cartilage
Cartilage is a nonedible byproduct with little saleable value. However, previous studies have proposed the possibility of producing peptides from cartilage with immune function modulation potential. The current study aimed to investigate the potential anti-inflammatory activity of peptides derived f...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000824 |
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| author | Li Yuan Qian Chu Bei Yang Wei Zhang Quancai Sun Ruichang Gao |
| author_facet | Li Yuan Qian Chu Bei Yang Wei Zhang Quancai Sun Ruichang Gao |
| author_sort | Li Yuan |
| collection | DOAJ |
| description | Cartilage is a nonedible byproduct with little saleable value. However, previous studies have proposed the possibility of producing peptides from cartilage with immune function modulation potential. The current study aimed to investigate the potential anti-inflammatory activity of peptides derived from sturgeon (Acipenser schrenckii) cartilage in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Five peptide sequences, including four novel peptides, were identified from ethanol-soluble cartilage hydrolysates. Among these five peptides, LTGP, LLLE, LLEL and VGPAGPAGP reduced the production of nitric oxide (NO) and interleukin-6 (IL-6) while increasing interleukin-10 (IL-10) excretion. Transcriptome analysis suggested the inhibition of activated mitogen-activated protein kinase (MAPK) and interleukin-17 (IL-17) signaling pathways after LLEL intervention. MAPK, which is involved in the IL-17 signaling pathway, was further proved to be blocked by downregulating the phosphorylation of p38, extracellular-signal regulated protein kinase (ERK), and c-jun N-terminal kinase (JNK). This novel peptide offers an attractive approach to develop functional foods. |
| format | Article |
| id | doaj-art-60059d6476234f368e621bb052119970 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-60059d6476234f368e621bb0521199702025-02-03T05:55:57ZengTsinghua University PressFood Science and Human Wellness2213-45302023-11-0112621752183Purification and identification of anti-inflammatory peptides from sturgeon (Acipenser schrenckii) cartilageLi Yuan0Qian Chu1Bei Yang2Wei Zhang3Quancai Sun4Ruichang Gao5School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Corresponding author.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Bio-resources Key Laboratory of Shaanxi Province, School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China; Corresponding author.Cartilage is a nonedible byproduct with little saleable value. However, previous studies have proposed the possibility of producing peptides from cartilage with immune function modulation potential. The current study aimed to investigate the potential anti-inflammatory activity of peptides derived from sturgeon (Acipenser schrenckii) cartilage in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Five peptide sequences, including four novel peptides, were identified from ethanol-soluble cartilage hydrolysates. Among these five peptides, LTGP, LLLE, LLEL and VGPAGPAGP reduced the production of nitric oxide (NO) and interleukin-6 (IL-6) while increasing interleukin-10 (IL-10) excretion. Transcriptome analysis suggested the inhibition of activated mitogen-activated protein kinase (MAPK) and interleukin-17 (IL-17) signaling pathways after LLEL intervention. MAPK, which is involved in the IL-17 signaling pathway, was further proved to be blocked by downregulating the phosphorylation of p38, extracellular-signal regulated protein kinase (ERK), and c-jun N-terminal kinase (JNK). This novel peptide offers an attractive approach to develop functional foods.http://www.sciencedirect.com/science/article/pii/S2213453023000824Sturgeon cartilagePeptidesAnti-inflammatoryMAPK |
| spellingShingle | Li Yuan Qian Chu Bei Yang Wei Zhang Quancai Sun Ruichang Gao Purification and identification of anti-inflammatory peptides from sturgeon (Acipenser schrenckii) cartilage Food Science and Human Wellness Sturgeon cartilage Peptides Anti-inflammatory MAPK |
| title | Purification and identification of anti-inflammatory peptides from sturgeon (Acipenser schrenckii) cartilage |
| title_full | Purification and identification of anti-inflammatory peptides from sturgeon (Acipenser schrenckii) cartilage |
| title_fullStr | Purification and identification of anti-inflammatory peptides from sturgeon (Acipenser schrenckii) cartilage |
| title_full_unstemmed | Purification and identification of anti-inflammatory peptides from sturgeon (Acipenser schrenckii) cartilage |
| title_short | Purification and identification of anti-inflammatory peptides from sturgeon (Acipenser schrenckii) cartilage |
| title_sort | purification and identification of anti inflammatory peptides from sturgeon acipenser schrenckii cartilage |
| topic | Sturgeon cartilage Peptides Anti-inflammatory MAPK |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000824 |
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