DJ-1 as a potential biomarker for the early diagnosis in lung cancer patients
DJ-1 is a novel oncogene that can transform NIH3T3 cells in cooperation with the activated ras gene. DJ-1 appears to have its greatest effect on tumourigenesis, and it may have a greater impact on early-stage lung cancers. In this study, we proposed to investigate the clinical value of DJ-1 protein...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-06-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317714625 |
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| _version_ | 1850057070075707392 |
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| author | Binbin Han Jiwen Wang Jia Gao Shana Feng Yu Zhu Xuexiang Li Ting Xiao Jun Qi Wei Cui |
| author_facet | Binbin Han Jiwen Wang Jia Gao Shana Feng Yu Zhu Xuexiang Li Ting Xiao Jun Qi Wei Cui |
| author_sort | Binbin Han |
| collection | DOAJ |
| description | DJ-1 is a novel oncogene that can transform NIH3T3 cells in cooperation with the activated ras gene. DJ-1 appears to have its greatest effect on tumourigenesis, and it may have a greater impact on early-stage lung cancers. In this study, we proposed to investigate the clinical value of DJ-1 protein in the early diagnosis of lung cancer and compared its diagnostic value with other biomarkers. Preoperative serum DJ-1 levels were measured in 300 lung cancer patients and compared with benign pulmonary disease (n = 44) and healthy volunteers (n = 64). Using tissue microarrays and immunohistochemical analyses, we compared the DJ-1 expression between the primary squamous cell carcinoma tumours and matched metastatic tissues from a lymph node. The baseline preoperative serum DJ-1 of lung cancer patients was significantly higher than that of benign diseases and healthy controls (p < 0.001). In the early-stage subgroup, the median DJ-1 concentration (ng/mL) was significantly higher than that of the advanced stage (12.90 vs 7.75, p < 0.05). Using immunohistochemistry, we observed that the DJ-1 staining intensity was generally weaker and less common in the metastatic tissues compared with that in the primary tumour (McNemar–Bowker Test, p = 0.008). DJ-1 was highly expressed in the early stage of lung cancer, and its expression was significantly decreased after metastasis. Therefore, DJ-1 may be a potential biomarker for the early diagnosis and monitoring of lung cancer metastasis. |
| format | Article |
| id | doaj-art-5ffd93afb02c49baae80ad28280107f4 |
| institution | DOAJ |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-5ffd93afb02c49baae80ad28280107f42025-08-20T02:51:31ZengSAGE PublishingTumor Biology1423-03802017-06-013910.1177/1010428317714625DJ-1 as a potential biomarker for the early diagnosis in lung cancer patientsBinbin Han0Jiwen Wang1Jia Gao2Shana Feng3Yu Zhu4Xuexiang Li5Ting Xiao6Jun Qi7Wei Cui8Department of Clinical Laboratory, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, ChinaDepartment of Thoracic Surgery, Zhejiang Cancer Hospital, Hangzhou, ChinaDepartment of Clinical Laboratory, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, ChinaDepartment of Clinical Laboratory, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, ChinaDepartment of Clinical Laboratory, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, ChinaDepartment of Clinical Laboratory, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, ChinaState Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, National Cancer Center/ Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaDepartment of Clinical Laboratory, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, ChinaDepartment of Clinical Laboratory, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, ChinaDJ-1 is a novel oncogene that can transform NIH3T3 cells in cooperation with the activated ras gene. DJ-1 appears to have its greatest effect on tumourigenesis, and it may have a greater impact on early-stage lung cancers. In this study, we proposed to investigate the clinical value of DJ-1 protein in the early diagnosis of lung cancer and compared its diagnostic value with other biomarkers. Preoperative serum DJ-1 levels were measured in 300 lung cancer patients and compared with benign pulmonary disease (n = 44) and healthy volunteers (n = 64). Using tissue microarrays and immunohistochemical analyses, we compared the DJ-1 expression between the primary squamous cell carcinoma tumours and matched metastatic tissues from a lymph node. The baseline preoperative serum DJ-1 of lung cancer patients was significantly higher than that of benign diseases and healthy controls (p < 0.001). In the early-stage subgroup, the median DJ-1 concentration (ng/mL) was significantly higher than that of the advanced stage (12.90 vs 7.75, p < 0.05). Using immunohistochemistry, we observed that the DJ-1 staining intensity was generally weaker and less common in the metastatic tissues compared with that in the primary tumour (McNemar–Bowker Test, p = 0.008). DJ-1 was highly expressed in the early stage of lung cancer, and its expression was significantly decreased after metastasis. Therefore, DJ-1 may be a potential biomarker for the early diagnosis and monitoring of lung cancer metastasis.https://doi.org/10.1177/1010428317714625 |
| spellingShingle | Binbin Han Jiwen Wang Jia Gao Shana Feng Yu Zhu Xuexiang Li Ting Xiao Jun Qi Wei Cui DJ-1 as a potential biomarker for the early diagnosis in lung cancer patients Tumor Biology |
| title | DJ-1 as a potential biomarker for the early diagnosis in lung cancer patients |
| title_full | DJ-1 as a potential biomarker for the early diagnosis in lung cancer patients |
| title_fullStr | DJ-1 as a potential biomarker for the early diagnosis in lung cancer patients |
| title_full_unstemmed | DJ-1 as a potential biomarker for the early diagnosis in lung cancer patients |
| title_short | DJ-1 as a potential biomarker for the early diagnosis in lung cancer patients |
| title_sort | dj 1 as a potential biomarker for the early diagnosis in lung cancer patients |
| url | https://doi.org/10.1177/1010428317714625 |
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