Novel Identification of CD74 as a Biomarker for Diagnosing and Prognosing Sepsis Patients
Kaibo Hu,1,2,* Ao Shi,3,* Yuan Shu,1,2 Shivon Sudesh,3 Jitao Ling,1,2 Yixuan Chen,2,4 Fuzhou Hua,4 Shuchun Yu,4 Jing Zhang,4 Peng Yu1 1Department of Endocrinology and Metabolism, second Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China; 2The second Clin...
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Dove Medical Press
2025-03-01
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| Series: | Journal of Inflammation Research |
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| Online Access: | https://www.dovepress.com/novel-identification-of-cd74-as-a-biomarker-for-diagnosing-and-prognos-peer-reviewed-fulltext-article-JIR |
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| author | Hu K Shi A Shu Y Sudesh S Ling J Chen Y Hua F Yu S Zhang J Yu P |
| author_facet | Hu K Shi A Shu Y Sudesh S Ling J Chen Y Hua F Yu S Zhang J Yu P |
| author_sort | Hu K |
| collection | DOAJ |
| description | Kaibo Hu,1,2,* Ao Shi,3,* Yuan Shu,1,2 Shivon Sudesh,3 Jitao Ling,1,2 Yixuan Chen,2,4 Fuzhou Hua,4 Shuchun Yu,4 Jing Zhang,4 Peng Yu1 1Department of Endocrinology and Metabolism, second Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China; 2The second Clinical Medical College, Nanchang University, Nanchang, People’s Republic of China; 3Faculty of Medicine, St George’s University of London, London, UK; 4Department of Anesthesiology, second Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Peng Yu, Email zhangyuteam2022@163.comPurpose: Sepsis, a life-threatening inflammatory condition due to an imbalanced response to infections, has been a major concern. Necroptosis, a newly discovered programmed cell death form, plays a crucial role in various inflammatory diseases. Our study aims to identify necroptosis - related genes (NRGs) and explore their potential for sepsis diagnosis.Patients and methods: We used weighted gene co-expression network analysis to identify gene modules associated with sepsis. Cox regression and Kaplan-Meier methods were employed to assess the diagnostic and prognostic value of these genes. Single-cell and immune infiltration analyses were carried out to explore the immune environment in sepsis. Plasma CD74 protein levels were quantified in our samples, and relevant clinical data from electronic patient records were analyzed for correlation.Results: CD74 was identified through the intersection of the hub genes of sepsis and NRGs related modules. Septic patients had lower CD74 expression compared to healthy controls. The CD74-based diagnostic model showed better performance in the training dataset (AUC, 0.79 [95% CI, 0.75– 0.84]), was cross-validated in external datasets, and demonstrated better performances than other published diagnostic models. Pathway analysis and single-cell profiling supported further exploration of CD74-related inflammation and immune response in sepsis.Conclusion: This study presents the first quantitative assessment of human plasma CD74 in sepsis patients. CD74 levels were significantly lower in the sepsis cohort. CD74 warrants further exploration as a potential prognostic and therapeutic target for sepsis. Keywords: CD74, immunity, diagnosis, sepsis, prognosis |
| format | Article |
| id | doaj-art-5ff59a347e2a4fd1886c4753db9ac9d7 |
| institution | DOAJ |
| issn | 1178-7031 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj-art-5ff59a347e2a4fd1886c4753db9ac9d72025-08-20T03:01:39ZengDove Medical PressJournal of Inflammation Research1178-70312025-03-01Volume 1838293842101148Novel Identification of CD74 as a Biomarker for Diagnosing and Prognosing Sepsis PatientsHu KShi AShu YSudesh SLing JChen YHua FYu SZhang JYu PKaibo Hu,1,2,* Ao Shi,3,* Yuan Shu,1,2 Shivon Sudesh,3 Jitao Ling,1,2 Yixuan Chen,2,4 Fuzhou Hua,4 Shuchun Yu,4 Jing Zhang,4 Peng Yu1 1Department of Endocrinology and Metabolism, second Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China; 2The second Clinical Medical College, Nanchang University, Nanchang, People’s Republic of China; 3Faculty of Medicine, St George’s University of London, London, UK; 4Department of Anesthesiology, second Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Peng Yu, Email zhangyuteam2022@163.comPurpose: Sepsis, a life-threatening inflammatory condition due to an imbalanced response to infections, has been a major concern. Necroptosis, a newly discovered programmed cell death form, plays a crucial role in various inflammatory diseases. Our study aims to identify necroptosis - related genes (NRGs) and explore their potential for sepsis diagnosis.Patients and methods: We used weighted gene co-expression network analysis to identify gene modules associated with sepsis. Cox regression and Kaplan-Meier methods were employed to assess the diagnostic and prognostic value of these genes. Single-cell and immune infiltration analyses were carried out to explore the immune environment in sepsis. Plasma CD74 protein levels were quantified in our samples, and relevant clinical data from electronic patient records were analyzed for correlation.Results: CD74 was identified through the intersection of the hub genes of sepsis and NRGs related modules. Septic patients had lower CD74 expression compared to healthy controls. The CD74-based diagnostic model showed better performance in the training dataset (AUC, 0.79 [95% CI, 0.75– 0.84]), was cross-validated in external datasets, and demonstrated better performances than other published diagnostic models. Pathway analysis and single-cell profiling supported further exploration of CD74-related inflammation and immune response in sepsis.Conclusion: This study presents the first quantitative assessment of human plasma CD74 in sepsis patients. CD74 levels were significantly lower in the sepsis cohort. CD74 warrants further exploration as a potential prognostic and therapeutic target for sepsis. Keywords: CD74, immunity, diagnosis, sepsis, prognosishttps://www.dovepress.com/novel-identification-of-cd74-as-a-biomarker-for-diagnosing-and-prognos-peer-reviewed-fulltext-article-JIRcd74immunitydiagnosissepsisprognosis |
| spellingShingle | Hu K Shi A Shu Y Sudesh S Ling J Chen Y Hua F Yu S Zhang J Yu P Novel Identification of CD74 as a Biomarker for Diagnosing and Prognosing Sepsis Patients Journal of Inflammation Research cd74 immunity diagnosis sepsis prognosis |
| title | Novel Identification of CD74 as a Biomarker for Diagnosing and Prognosing Sepsis Patients |
| title_full | Novel Identification of CD74 as a Biomarker for Diagnosing and Prognosing Sepsis Patients |
| title_fullStr | Novel Identification of CD74 as a Biomarker for Diagnosing and Prognosing Sepsis Patients |
| title_full_unstemmed | Novel Identification of CD74 as a Biomarker for Diagnosing and Prognosing Sepsis Patients |
| title_short | Novel Identification of CD74 as a Biomarker for Diagnosing and Prognosing Sepsis Patients |
| title_sort | novel identification of cd74 as a biomarker for diagnosing and prognosing sepsis patients |
| topic | cd74 immunity diagnosis sepsis prognosis |
| url | https://www.dovepress.com/novel-identification-of-cd74-as-a-biomarker-for-diagnosing-and-prognos-peer-reviewed-fulltext-article-JIR |
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