Volatile Metabolome and Transcriptomic Analysis of <i>Kosakonia cowanii</i> Ch1 During Competitive Interaction with <i>Sclerotium rolfsii</i> Reveals New Biocontrol Insights
The volatile organic compounds (VOCs) produced by <i>K. cowanii</i> Ch1 play a significant role in the inhibition of the mycelial growth of phytopathogen strains. As a continuation of our previous studies, we aim to elucidate the mechanisms of the responses of <i>K. cowanii</i&g...
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2025-06-01
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| author | Yoali Fernanda Hernández Gómez Jacqueline González Espinosa Griselda Catalina Olvera Rivas Jackeline Lizzeta Arvizu Gómez José Humberto Valenzuela Soto Miguel Angel Ramos López Aldo Amaro Reyes Eloy Rodríguez de León Carlos Saldaña José Luis Hernández Flores Juan Campos Guillén |
| author_facet | Yoali Fernanda Hernández Gómez Jacqueline González Espinosa Griselda Catalina Olvera Rivas Jackeline Lizzeta Arvizu Gómez José Humberto Valenzuela Soto Miguel Angel Ramos López Aldo Amaro Reyes Eloy Rodríguez de León Carlos Saldaña José Luis Hernández Flores Juan Campos Guillén |
| author_sort | Yoali Fernanda Hernández Gómez |
| collection | DOAJ |
| description | The volatile organic compounds (VOCs) produced by <i>K. cowanii</i> Ch1 play a significant role in the inhibition of the mycelial growth of phytopathogen strains. As a continuation of our previous studies, we aim to elucidate the mechanisms of the responses of <i>K. cowanii</i> Ch1 against <i>S. rolfsii</i> during a colonization competence interaction in the presence and absence of a mixture of bacterial VOCs under in vitro conditions. The results of this study showed that, in the absence of bacterial VOCs, <i>K. cowanii</i> Ch1 cannot compete against <i>S. rolfsii</i>, and the RNA-Seq analysis revealed the differential expression of genes related to the oxidative stress response in <i>K. cowanii</i> Ch1 for survival. However, in the presence of bacterial VOCs, an interesting phenotypical response was observed in <i>K. cowanii</i> Ch1, resulting in the mycelial growth inhibition of <i>S. rolfsii</i>. The upregulated genes were related to the siderophore-mediated iron transport system, zinc ion transport system, antibiotic biosynthesis monooxygenase, carbohydrate metabolism, polyketide synthase modules, and related proteins, and <i>katG</i> was probably related to the phenotype resulting in the formation of gas bubbles by <i>K. cowanii</i>. In addition, the VOC profile analyzed at 36 h for bacterial growth revealed a cocktail with an ability to increase the competence of <i>K. cowanii</i> Ch1 against <i>S. rolfsii</i> in vitro and in vivo. This study provides evidence regarding the key role that VOCs play during the colonization competition involving <i>K. cowanii</i> Ch1, the comprehension of which may enable the development of new biocontrol strategies. |
| format | Article |
| id | doaj-art-5ff31465666541b09e815097aebd24f9 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-5ff31465666541b09e815097aebd24f92025-08-20T02:47:10ZengMDPI AGMicroorganisms2076-26072025-06-01137148310.3390/microorganisms13071483Volatile Metabolome and Transcriptomic Analysis of <i>Kosakonia cowanii</i> Ch1 During Competitive Interaction with <i>Sclerotium rolfsii</i> Reveals New Biocontrol InsightsYoali Fernanda Hernández Gómez0Jacqueline González Espinosa1Griselda Catalina Olvera Rivas2Jackeline Lizzeta Arvizu Gómez3José Humberto Valenzuela Soto4Miguel Angel Ramos López5Aldo Amaro Reyes6Eloy Rodríguez de León7Carlos Saldaña8José Luis Hernández Flores9Juan Campos Guillén10Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Querétaro 76010, MexicoFacultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Querétaro 76010, MexicoFacultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Querétaro 76010, MexicoSecretaría de Investigación y Posgrado, Centro Nayarita de Innovación y Transferencia de Tecnología (CENITT), Universidad Autónoma de Nayarit, Tepic 63173, MexicoCentro de Investigación en Química Aplicada, Blvd. Enrique Reyna Hermosillo No. 140, Saltillo 25294, MexicoFacultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Querétaro 76010, MexicoFacultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Querétaro 76010, MexicoFacultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Querétaro 76010, MexicoFacultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Av. De las Ciencias S/N, Querétaro 76220, MexicoCentro de Investigación y de Estudios Avanzados del IPN, Irapuato 36824, MexicoFacultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Querétaro 76010, MexicoThe volatile organic compounds (VOCs) produced by <i>K. cowanii</i> Ch1 play a significant role in the inhibition of the mycelial growth of phytopathogen strains. As a continuation of our previous studies, we aim to elucidate the mechanisms of the responses of <i>K. cowanii</i> Ch1 against <i>S. rolfsii</i> during a colonization competence interaction in the presence and absence of a mixture of bacterial VOCs under in vitro conditions. The results of this study showed that, in the absence of bacterial VOCs, <i>K. cowanii</i> Ch1 cannot compete against <i>S. rolfsii</i>, and the RNA-Seq analysis revealed the differential expression of genes related to the oxidative stress response in <i>K. cowanii</i> Ch1 for survival. However, in the presence of bacterial VOCs, an interesting phenotypical response was observed in <i>K. cowanii</i> Ch1, resulting in the mycelial growth inhibition of <i>S. rolfsii</i>. The upregulated genes were related to the siderophore-mediated iron transport system, zinc ion transport system, antibiotic biosynthesis monooxygenase, carbohydrate metabolism, polyketide synthase modules, and related proteins, and <i>katG</i> was probably related to the phenotype resulting in the formation of gas bubbles by <i>K. cowanii</i>. In addition, the VOC profile analyzed at 36 h for bacterial growth revealed a cocktail with an ability to increase the competence of <i>K. cowanii</i> Ch1 against <i>S. rolfsii</i> in vitro and in vivo. This study provides evidence regarding the key role that VOCs play during the colonization competition involving <i>K. cowanii</i> Ch1, the comprehension of which may enable the development of new biocontrol strategies.https://www.mdpi.com/2076-2607/13/7/1483<i>K. cowanii</i> Ch1<i>S. rolfsii</i>RNA-Seqcolonization competenceVOC profilebiocontrol |
| spellingShingle | Yoali Fernanda Hernández Gómez Jacqueline González Espinosa Griselda Catalina Olvera Rivas Jackeline Lizzeta Arvizu Gómez José Humberto Valenzuela Soto Miguel Angel Ramos López Aldo Amaro Reyes Eloy Rodríguez de León Carlos Saldaña José Luis Hernández Flores Juan Campos Guillén Volatile Metabolome and Transcriptomic Analysis of <i>Kosakonia cowanii</i> Ch1 During Competitive Interaction with <i>Sclerotium rolfsii</i> Reveals New Biocontrol Insights Microorganisms <i>K. cowanii</i> Ch1 <i>S. rolfsii</i> RNA-Seq colonization competence VOC profile biocontrol |
| title | Volatile Metabolome and Transcriptomic Analysis of <i>Kosakonia cowanii</i> Ch1 During Competitive Interaction with <i>Sclerotium rolfsii</i> Reveals New Biocontrol Insights |
| title_full | Volatile Metabolome and Transcriptomic Analysis of <i>Kosakonia cowanii</i> Ch1 During Competitive Interaction with <i>Sclerotium rolfsii</i> Reveals New Biocontrol Insights |
| title_fullStr | Volatile Metabolome and Transcriptomic Analysis of <i>Kosakonia cowanii</i> Ch1 During Competitive Interaction with <i>Sclerotium rolfsii</i> Reveals New Biocontrol Insights |
| title_full_unstemmed | Volatile Metabolome and Transcriptomic Analysis of <i>Kosakonia cowanii</i> Ch1 During Competitive Interaction with <i>Sclerotium rolfsii</i> Reveals New Biocontrol Insights |
| title_short | Volatile Metabolome and Transcriptomic Analysis of <i>Kosakonia cowanii</i> Ch1 During Competitive Interaction with <i>Sclerotium rolfsii</i> Reveals New Biocontrol Insights |
| title_sort | volatile metabolome and transcriptomic analysis of i kosakonia cowanii i ch1 during competitive interaction with i sclerotium rolfsii i reveals new biocontrol insights |
| topic | <i>K. cowanii</i> Ch1 <i>S. rolfsii</i> RNA-Seq colonization competence VOC profile biocontrol |
| url | https://www.mdpi.com/2076-2607/13/7/1483 |
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