Biodegraded hay with graded addition of Pleurotus ostreatus improves dry matter disappearance and reduces methane production of diets incubated in vitro
Bioconversion of plant material with white-rot fungi has demonstrated improvements in in vitro digestibility, however, effects on rumen fermentation parameters and methane (CH4) production, when included as a dietary ingredient, are not clear. The aim of this study was to assess changes in rumen fer...
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| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Italian Journal of Animal Science |
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| Online Access: | http://dx.doi.org/10.1080/1828051X.2023.2193607 |
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| author | Rita Astudillo-Neira Sandra Suescun-Ospina Nelson Vera-Aguilera Julio Alarcon-Enos Jorge Ávila-Stagno |
| author_facet | Rita Astudillo-Neira Sandra Suescun-Ospina Nelson Vera-Aguilera Julio Alarcon-Enos Jorge Ávila-Stagno |
| author_sort | Rita Astudillo-Neira |
| collection | DOAJ |
| description | Bioconversion of plant material with white-rot fungi has demonstrated improvements in in vitro digestibility, however, effects on rumen fermentation parameters and methane (CH4) production, when included as a dietary ingredient, are not clear. The aim of this study was to assess changes in rumen fermentation parameters and in vitro CH4 production by dietary inclusion of ryegrass-fescue hay biodegraded with P. ostreatus (H-PO) during 14 days of solid fermentation. The experiment used 2 diets with contrasting acid detergent fibre (aADFom) content which were added with 0, 3 and 6% H-PO. Substrates were incubated 3 times with sampling at 24 h. Results were analysed by ANOVA, and compared with Tukey’s test and polynomial contrasts. Inclusion of H-PO at concentrations of 3 and 6% resulted in increased in vitro dry matter (DM) disappearance (IVDMD) by 10 and 12% respectively, whereas high concentrations of aADFom reduced IVDMD by 10% as compared to low aADFom. Methane production (mg/g DM incubated) and yield (mg/g DM degraded) were reduced by increasing aADFom and P. ostreatus biodegraded hay concentrations in the diets (p < 0.001) whereas concentrations of total short-chain fatty acids were not affected by the inclusion of biodegraded hay (p = 0.080) or aADFom concentration (p = 0.076). Increasing concentrations of biodegraded hay resulted in increased acetate (p < 0.001), and reduced propionate proportions (p = 0.021) but did not affect butyrate (p = 0.233). In conclusion the use of biodegraded hay as a pre-treated ingredient improved the utilisation of low and high fibre diets by increasing the in vitro disappearance, and decreasing CH4 emissions, without increasing the propionate proportion.HIGHLIGHTS The use of hay biodegraded with P. ostreatus (H-PO) improves the digestibility of ruminant diets. The use of H-PO decreases methane, and increases acetate production in in vitro conditions. The use of H-PO as an ingredient in ruminant diets could improve the sustainability of livestock systems. |
| format | Article |
| id | doaj-art-5ff06765283e4d89841b4f4f43e682d6 |
| institution | OA Journals |
| issn | 1594-4077 1828-051X |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
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| series | Italian Journal of Animal Science |
| spelling | doaj-art-5ff06765283e4d89841b4f4f43e682d62025-08-20T02:07:48ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2023-12-0122134735810.1080/1828051X.2023.21936072193607Biodegraded hay with graded addition of Pleurotus ostreatus improves dry matter disappearance and reduces methane production of diets incubated in vitroRita Astudillo-Neira0Sandra Suescun-Ospina1Nelson Vera-Aguilera2Julio Alarcon-Enos3Jorge Ávila-Stagno4Laboratorio de Nutrición y Sistemas Ganaderos. Departamento de Ciencia Animal, Universidad de ConcepciónLaboratorio de Nutrición y Sistemas Ganaderos. Departamento de Ciencia Animal, Universidad de ConcepciónLaboratorio de Nutrición y Sistemas Ganaderos. Departamento de Ciencia Animal, Universidad de ConcepciónLaboratorio de Síntesis y Biotransformación de Productos Naturales. Departamento de Ciencias Básicas, Universidad del Bio BioLaboratorio de Nutrición y Sistemas Ganaderos. Departamento de Ciencia Animal, Universidad de ConcepciónBioconversion of plant material with white-rot fungi has demonstrated improvements in in vitro digestibility, however, effects on rumen fermentation parameters and methane (CH4) production, when included as a dietary ingredient, are not clear. The aim of this study was to assess changes in rumen fermentation parameters and in vitro CH4 production by dietary inclusion of ryegrass-fescue hay biodegraded with P. ostreatus (H-PO) during 14 days of solid fermentation. The experiment used 2 diets with contrasting acid detergent fibre (aADFom) content which were added with 0, 3 and 6% H-PO. Substrates were incubated 3 times with sampling at 24 h. Results were analysed by ANOVA, and compared with Tukey’s test and polynomial contrasts. Inclusion of H-PO at concentrations of 3 and 6% resulted in increased in vitro dry matter (DM) disappearance (IVDMD) by 10 and 12% respectively, whereas high concentrations of aADFom reduced IVDMD by 10% as compared to low aADFom. Methane production (mg/g DM incubated) and yield (mg/g DM degraded) were reduced by increasing aADFom and P. ostreatus biodegraded hay concentrations in the diets (p < 0.001) whereas concentrations of total short-chain fatty acids were not affected by the inclusion of biodegraded hay (p = 0.080) or aADFom concentration (p = 0.076). Increasing concentrations of biodegraded hay resulted in increased acetate (p < 0.001), and reduced propionate proportions (p = 0.021) but did not affect butyrate (p = 0.233). In conclusion the use of biodegraded hay as a pre-treated ingredient improved the utilisation of low and high fibre diets by increasing the in vitro disappearance, and decreasing CH4 emissions, without increasing the propionate proportion.HIGHLIGHTS The use of hay biodegraded with P. ostreatus (H-PO) improves the digestibility of ruminant diets. The use of H-PO decreases methane, and increases acetate production in in vitro conditions. The use of H-PO as an ingredient in ruminant diets could improve the sustainability of livestock systems.http://dx.doi.org/10.1080/1828051X.2023.2193607basidiomycetesruminant nutritionsolid fermentationgreenhouse gasesmitigation |
| spellingShingle | Rita Astudillo-Neira Sandra Suescun-Ospina Nelson Vera-Aguilera Julio Alarcon-Enos Jorge Ávila-Stagno Biodegraded hay with graded addition of Pleurotus ostreatus improves dry matter disappearance and reduces methane production of diets incubated in vitro Italian Journal of Animal Science basidiomycetes ruminant nutrition solid fermentation greenhouse gases mitigation |
| title | Biodegraded hay with graded addition of Pleurotus ostreatus improves dry matter disappearance and reduces methane production of diets incubated in vitro |
| title_full | Biodegraded hay with graded addition of Pleurotus ostreatus improves dry matter disappearance and reduces methane production of diets incubated in vitro |
| title_fullStr | Biodegraded hay with graded addition of Pleurotus ostreatus improves dry matter disappearance and reduces methane production of diets incubated in vitro |
| title_full_unstemmed | Biodegraded hay with graded addition of Pleurotus ostreatus improves dry matter disappearance and reduces methane production of diets incubated in vitro |
| title_short | Biodegraded hay with graded addition of Pleurotus ostreatus improves dry matter disappearance and reduces methane production of diets incubated in vitro |
| title_sort | biodegraded hay with graded addition of pleurotus ostreatus improves dry matter disappearance and reduces methane production of diets incubated in vitro |
| topic | basidiomycetes ruminant nutrition solid fermentation greenhouse gases mitigation |
| url | http://dx.doi.org/10.1080/1828051X.2023.2193607 |
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