Impact of Genetic Variants on Pregabalin Pharmacokinetics and Safety

<b>Background/Objectives:</b> Pregabalin is a useful therapeutic option for patients with anxiety or neuropathic pain. Genetic variants in certain genes encoding for transporters related to absorption and distribution could have an impact on the efficacy and safety of the drug. Furthermo...

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Main Authors: Sofía Calleja, Andrea Rodríguez-López, Dolores Ochoa, Sergio Luquero, Marcos Navares-Gómez, Manuel Román, Gina Mejia-Abril, Samuel Martín-Vilchez, Francisco Abad-Santos, Pablo Zubiaur
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Language:English
Published: MDPI AG 2025-01-01
Series:Pharmaceuticals
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Online Access:https://www.mdpi.com/1424-8247/18/2/151
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author Sofía Calleja
Andrea Rodríguez-López
Dolores Ochoa
Sergio Luquero
Marcos Navares-Gómez
Manuel Román
Gina Mejia-Abril
Samuel Martín-Vilchez
Francisco Abad-Santos
Pablo Zubiaur
author_facet Sofía Calleja
Andrea Rodríguez-López
Dolores Ochoa
Sergio Luquero
Marcos Navares-Gómez
Manuel Román
Gina Mejia-Abril
Samuel Martín-Vilchez
Francisco Abad-Santos
Pablo Zubiaur
author_sort Sofía Calleja
collection DOAJ
description <b>Background/Objectives:</b> Pregabalin is a useful therapeutic option for patients with anxiety or neuropathic pain. Genetic variants in certain genes encoding for transporters related to absorption and distribution could have an impact on the efficacy and safety of the drug. Furthermore, extreme phenotypes in metabolic enzymes could alter pregabalin-limited metabolism. <b>Methods</b>: In this study, we included 24 healthy volunteers participating in a bioequivalence clinical trial and administered pregabalin 300 mg orally; 23 subjects were genotyped for 114 variants in 31 candidate genes, and we explored their impact on pregabalin pharmacokinetics and safety. <b>Results</b>: The uncorrected mean (±SD) of AUC<sub>∞</sub> and C<sub>max</sub> were 61,097 ± 14,762 ng*h/mL and 7802 ± 1659 ng/mL, respectively, which were significantly higher in females than in males (<i>p</i> = 0.002 and <i>p</i> = 0.001, respectively), with no differences in dose/weight (DW)- corrected exposure metrics. NAT2 slow acetylators (SAs) showed a 16–18% increase in exposure compared to intermediate (IAs) and normal (NAs) acetylators; NAT2 SAs exhibited a 25% higher t<sub>1/2</sub> as compared with NAT2 IAs and 58% higher compared to NAT2 NAs. In contrast, neither the NAT2 phenotype nor other genetic variants were related to pregabalin adverse drug reaction (ADR) occurrence. On the contrary, sex and sex-related exposure differences (i.e., females and their higher exposure compared to males) were the main predictors of ADR occurrence. <b>Conclusions</b>: Our findings suggest that NAT2 could be partially responsible for the minor proportion of pregabalin metabolism, but the effect of NAT2 phenotype does not seem clinically relevant. Therefore, pharmacogenetic biomarkers appear to play a restrained role in pregabalin pharmacotherapy.
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spelling doaj-art-5fec6c8d5a7a4afabc64343b68cc99f12025-08-20T03:12:05ZengMDPI AGPharmaceuticals1424-82472025-01-0118215110.3390/ph18020151Impact of Genetic Variants on Pregabalin Pharmacokinetics and SafetySofía Calleja0Andrea Rodríguez-López1Dolores Ochoa2Sergio Luquero3Marcos Navares-Gómez4Manuel Román5Gina Mejia-Abril6Samuel Martín-Vilchez7Francisco Abad-Santos8Pablo Zubiaur9Clinical Pharmacology Department, Hospital Universitario de La Princesa, Faculty of Medicine, Universidad Autónoma de Madrid (UAM), Instituto de Investigación Sanitaria La Princesa (IIS-Princesa), 28006 Madrid, SpainClinical Pharmacology Department, Hospital Universitario de La Princesa, Faculty of Medicine, Universidad Autónoma de Madrid (UAM), Instituto de Investigación Sanitaria La Princesa (IIS-Princesa), 28006 Madrid, SpainClinical Pharmacology Department, Hospital Universitario de La Princesa, Faculty of Medicine, Universidad Autónoma de Madrid (UAM), Instituto de Investigación Sanitaria La Princesa (IIS-Princesa), 28006 Madrid, SpainClinical Pharmacology Department, Hospital Universitario de La Princesa, Faculty of Medicine, Universidad Autónoma de Madrid (UAM), Instituto de Investigación Sanitaria La Princesa (IIS-Princesa), 28006 Madrid, SpainClinical Pharmacology Department, Hospital Universitario de La Princesa, Faculty of Medicine, Universidad Autónoma de Madrid (UAM), Instituto de Investigación Sanitaria La Princesa (IIS-Princesa), 28006 Madrid, SpainClinical Pharmacology Department, Hospital Universitario de La Princesa, Faculty of Medicine, Universidad Autónoma de Madrid (UAM), Instituto de Investigación Sanitaria La Princesa (IIS-Princesa), 28006 Madrid, SpainClinical Pharmacology Department, Hospital Universitario de La Princesa, Faculty of Medicine, Universidad Autónoma de Madrid (UAM), Instituto de Investigación Sanitaria La Princesa (IIS-Princesa), 28006 Madrid, SpainClinical Pharmacology Department, Hospital Universitario de La Princesa, Faculty of Medicine, Universidad Autónoma de Madrid (UAM), Instituto de Investigación Sanitaria La Princesa (IIS-Princesa), 28006 Madrid, SpainClinical Pharmacology Department, Hospital Universitario de La Princesa, Faculty of Medicine, Universidad Autónoma de Madrid (UAM), Instituto de Investigación Sanitaria La Princesa (IIS-Princesa), 28006 Madrid, SpainClinical Pharmacology Department, Hospital Universitario de La Princesa, Faculty of Medicine, Universidad Autónoma de Madrid (UAM), Instituto de Investigación Sanitaria La Princesa (IIS-Princesa), 28006 Madrid, Spain<b>Background/Objectives:</b> Pregabalin is a useful therapeutic option for patients with anxiety or neuropathic pain. Genetic variants in certain genes encoding for transporters related to absorption and distribution could have an impact on the efficacy and safety of the drug. Furthermore, extreme phenotypes in metabolic enzymes could alter pregabalin-limited metabolism. <b>Methods</b>: In this study, we included 24 healthy volunteers participating in a bioequivalence clinical trial and administered pregabalin 300 mg orally; 23 subjects were genotyped for 114 variants in 31 candidate genes, and we explored their impact on pregabalin pharmacokinetics and safety. <b>Results</b>: The uncorrected mean (±SD) of AUC<sub>∞</sub> and C<sub>max</sub> were 61,097 ± 14,762 ng*h/mL and 7802 ± 1659 ng/mL, respectively, which were significantly higher in females than in males (<i>p</i> = 0.002 and <i>p</i> = 0.001, respectively), with no differences in dose/weight (DW)- corrected exposure metrics. NAT2 slow acetylators (SAs) showed a 16–18% increase in exposure compared to intermediate (IAs) and normal (NAs) acetylators; NAT2 SAs exhibited a 25% higher t<sub>1/2</sub> as compared with NAT2 IAs and 58% higher compared to NAT2 NAs. In contrast, neither the NAT2 phenotype nor other genetic variants were related to pregabalin adverse drug reaction (ADR) occurrence. On the contrary, sex and sex-related exposure differences (i.e., females and their higher exposure compared to males) were the main predictors of ADR occurrence. <b>Conclusions</b>: Our findings suggest that NAT2 could be partially responsible for the minor proportion of pregabalin metabolism, but the effect of NAT2 phenotype does not seem clinically relevant. Therefore, pharmacogenetic biomarkers appear to play a restrained role in pregabalin pharmacotherapy.https://www.mdpi.com/1424-8247/18/2/151pregabalinpharmacokineticssafetypharmacogenetics
spellingShingle Sofía Calleja
Andrea Rodríguez-López
Dolores Ochoa
Sergio Luquero
Marcos Navares-Gómez
Manuel Román
Gina Mejia-Abril
Samuel Martín-Vilchez
Francisco Abad-Santos
Pablo Zubiaur
Impact of Genetic Variants on Pregabalin Pharmacokinetics and Safety
Pharmaceuticals
pregabalin
pharmacokinetics
safety
pharmacogenetics
title Impact of Genetic Variants on Pregabalin Pharmacokinetics and Safety
title_full Impact of Genetic Variants on Pregabalin Pharmacokinetics and Safety
title_fullStr Impact of Genetic Variants on Pregabalin Pharmacokinetics and Safety
title_full_unstemmed Impact of Genetic Variants on Pregabalin Pharmacokinetics and Safety
title_short Impact of Genetic Variants on Pregabalin Pharmacokinetics and Safety
title_sort impact of genetic variants on pregabalin pharmacokinetics and safety
topic pregabalin
pharmacokinetics
safety
pharmacogenetics
url https://www.mdpi.com/1424-8247/18/2/151
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