Antimicrobial resistance modifying effects and molecular docking studies of Affinine, derived from Tabernaemontana crassa
Tabernaemontana crassa is a native plant indigenous to Ghana which has garnered local recognition for its purported efficacy against ringworm, wounds, abscesses, and antibacterial infections. However, the antibacterial and resistance modifying effects of the crude extracts and isolated compounds hav...
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Elsevier
2024-12-01
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| Series: | Scientific African |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468227624003247 |
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| author | Denick Amon Prince Manu Evelyn Asante-Kwatia Priscilla Kolibea Mante Cynthia Amaning Danquah Lawrence Sheringham Borquaye Edmund Ekuadzi |
| author_facet | Denick Amon Prince Manu Evelyn Asante-Kwatia Priscilla Kolibea Mante Cynthia Amaning Danquah Lawrence Sheringham Borquaye Edmund Ekuadzi |
| author_sort | Denick Amon |
| collection | DOAJ |
| description | Tabernaemontana crassa is a native plant indigenous to Ghana which has garnered local recognition for its purported efficacy against ringworm, wounds, abscesses, and antibacterial infections. However, the antibacterial and resistance modifying effects of the crude extracts and isolated compounds have not been investigated. Consequently, this study aims to evaluate the antimicrobial and resistance modifying effects of the extracts and affinine, a monoterpene indole alkaloid, from T. crassa. Four extracts, namely the crude, alkaloidal, glycosidic and triterpene fractions were prepared by solvent-solvent extraction from the stem bark of T. crassa. Using chromatographic and spectroscopic techniques, affinine was isolated and identified from the alkaloidal fraction. The effects of the extracts and affinine were investigated using the micro dilution, efflux pump and biofilm inhibition assays against Staphylococcus aureus and Pseudomonas aeruginosa. The crude extracts were effective against the two bacteria strains at a concentration of 500 µg/ml. Affinine was without activity in the in vitro antimicrobial assays with MIC >1000 µg/ml. However, affinine possessed anti-efflux and antibiofilm activity against the test microorganisms which was confirmed with in silico studies. The study showed the efficacy of the extracts against the two microbes associated with wound healing. Affinine, not previously described from T. crassa, may contribute to the anti-infective effects of the plant. |
| format | Article |
| id | doaj-art-5febbf8d8f7b4c27b5dd806709f1bc40 |
| institution | OA Journals |
| issn | 2468-2276 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj-art-5febbf8d8f7b4c27b5dd806709f1bc402025-08-20T02:32:19ZengElsevierScientific African2468-22762024-12-0126e0238210.1016/j.sciaf.2024.e02382Antimicrobial resistance modifying effects and molecular docking studies of Affinine, derived from Tabernaemontana crassaDenick Amon0Prince Manu1Evelyn Asante-Kwatia2Priscilla Kolibea Mante3Cynthia Amaning Danquah4Lawrence Sheringham Borquaye5Edmund Ekuadzi6Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Chemistry, Faculty of Physical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Chemistry, Faculty of Physical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana; Corresponding author.Tabernaemontana crassa is a native plant indigenous to Ghana which has garnered local recognition for its purported efficacy against ringworm, wounds, abscesses, and antibacterial infections. However, the antibacterial and resistance modifying effects of the crude extracts and isolated compounds have not been investigated. Consequently, this study aims to evaluate the antimicrobial and resistance modifying effects of the extracts and affinine, a monoterpene indole alkaloid, from T. crassa. Four extracts, namely the crude, alkaloidal, glycosidic and triterpene fractions were prepared by solvent-solvent extraction from the stem bark of T. crassa. Using chromatographic and spectroscopic techniques, affinine was isolated and identified from the alkaloidal fraction. The effects of the extracts and affinine were investigated using the micro dilution, efflux pump and biofilm inhibition assays against Staphylococcus aureus and Pseudomonas aeruginosa. The crude extracts were effective against the two bacteria strains at a concentration of 500 µg/ml. Affinine was without activity in the in vitro antimicrobial assays with MIC >1000 µg/ml. However, affinine possessed anti-efflux and antibiofilm activity against the test microorganisms which was confirmed with in silico studies. The study showed the efficacy of the extracts against the two microbes associated with wound healing. Affinine, not previously described from T. crassa, may contribute to the anti-infective effects of the plant.http://www.sciencedirect.com/science/article/pii/S2468227624003247VobasanoneMonoterpene indole alkaloidAutodockMolecular dockingBiofilm inhibitionEfflux pump inhibition |
| spellingShingle | Denick Amon Prince Manu Evelyn Asante-Kwatia Priscilla Kolibea Mante Cynthia Amaning Danquah Lawrence Sheringham Borquaye Edmund Ekuadzi Antimicrobial resistance modifying effects and molecular docking studies of Affinine, derived from Tabernaemontana crassa Scientific African Vobasanone Monoterpene indole alkaloid Autodock Molecular docking Biofilm inhibition Efflux pump inhibition |
| title | Antimicrobial resistance modifying effects and molecular docking studies of Affinine, derived from Tabernaemontana crassa |
| title_full | Antimicrobial resistance modifying effects and molecular docking studies of Affinine, derived from Tabernaemontana crassa |
| title_fullStr | Antimicrobial resistance modifying effects and molecular docking studies of Affinine, derived from Tabernaemontana crassa |
| title_full_unstemmed | Antimicrobial resistance modifying effects and molecular docking studies of Affinine, derived from Tabernaemontana crassa |
| title_short | Antimicrobial resistance modifying effects and molecular docking studies of Affinine, derived from Tabernaemontana crassa |
| title_sort | antimicrobial resistance modifying effects and molecular docking studies of affinine derived from tabernaemontana crassa |
| topic | Vobasanone Monoterpene indole alkaloid Autodock Molecular docking Biofilm inhibition Efflux pump inhibition |
| url | http://www.sciencedirect.com/science/article/pii/S2468227624003247 |
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