The Hypoxia-Mimetic Agent Cobalt Chloride Differently Affects Human Mesenchymal Stem Cells in Their Chondrogenic Potential
Adult stem cells are a promising cell source for cartilage regeneration. They resided in a special microenvironment known as the stem-cell niche, characterized by the presence of low oxygen concentration. Cobalt chloride (CoCl2) imitates hypoxia in vitro by stabilizing hypoxia-inducible factor-alpha...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/3237253 |
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| author | Gabriella Teti Stefano Focaroli Viviana Salvatore Eleonora Mazzotti Laura Ingra’ Antonio Mazzotti Mirella Falconi |
| author_facet | Gabriella Teti Stefano Focaroli Viviana Salvatore Eleonora Mazzotti Laura Ingra’ Antonio Mazzotti Mirella Falconi |
| author_sort | Gabriella Teti |
| collection | DOAJ |
| description | Adult stem cells are a promising cell source for cartilage regeneration. They resided in a special microenvironment known as the stem-cell niche, characterized by the presence of low oxygen concentration. Cobalt chloride (CoCl2) imitates hypoxia in vitro by stabilizing hypoxia-inducible factor-alpha (HIF-1α), which is the master regulator in the cellular adaptive response to hypoxia. In this study, the influence of CoCl2 on the chondrogenic potential of human MSCs, isolated from dental pulp, umbilical cord, and adipose tissue, was investigated. Cells were treated with concentrations of CoCl2 ranging from 50 to 400 μM. Cell viability, HIF-1α protein synthesis, and the expression of the chondrogenic markers were analyzed. The results showed that the CoCl2 supplementation had no effect on cell viability, while the upregulation of chondrogenic markers such as SOX9, COL2A1, VCAN, and ACAN was dependent on the cellular source. This study shows that hypoxia, induced by CoCl2 treatment, can differently influence the behavior of MSCs, isolated from different sources, in their chondrogenic potential. These findings should be taken into consideration in the treatment of cartilage repair and regeneration based on stem cell therapies. |
| format | Article |
| id | doaj-art-5fe647e2f5a449b1bef36fa6a6c66c39 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-5fe647e2f5a449b1bef36fa6a6c66c392025-08-20T03:34:53ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/32372533237253The Hypoxia-Mimetic Agent Cobalt Chloride Differently Affects Human Mesenchymal Stem Cells in Their Chondrogenic PotentialGabriella Teti0Stefano Focaroli1Viviana Salvatore2Eleonora Mazzotti3Laura Ingra’4Antonio Mazzotti5Mirella Falconi6Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, ItalyDepartment of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, ItalyAn2H Discovery Limited, National Institute of Cellular Biotechnology (NICB), Dublin City University Campus, Dublin, IrelandFaculty of Comparative Biomedical Sciences, University of Teramo, Teramo, ItalyDepartment of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, ItalyFirst Orthopaedic and Traumatologic Clinic, Istituto Ortopedico Rizzoli, University of Bologna, Bologna, ItalyDepartment of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, ItalyAdult stem cells are a promising cell source for cartilage regeneration. They resided in a special microenvironment known as the stem-cell niche, characterized by the presence of low oxygen concentration. Cobalt chloride (CoCl2) imitates hypoxia in vitro by stabilizing hypoxia-inducible factor-alpha (HIF-1α), which is the master regulator in the cellular adaptive response to hypoxia. In this study, the influence of CoCl2 on the chondrogenic potential of human MSCs, isolated from dental pulp, umbilical cord, and adipose tissue, was investigated. Cells were treated with concentrations of CoCl2 ranging from 50 to 400 μM. Cell viability, HIF-1α protein synthesis, and the expression of the chondrogenic markers were analyzed. The results showed that the CoCl2 supplementation had no effect on cell viability, while the upregulation of chondrogenic markers such as SOX9, COL2A1, VCAN, and ACAN was dependent on the cellular source. This study shows that hypoxia, induced by CoCl2 treatment, can differently influence the behavior of MSCs, isolated from different sources, in their chondrogenic potential. These findings should be taken into consideration in the treatment of cartilage repair and regeneration based on stem cell therapies.http://dx.doi.org/10.1155/2018/3237253 |
| spellingShingle | Gabriella Teti Stefano Focaroli Viviana Salvatore Eleonora Mazzotti Laura Ingra’ Antonio Mazzotti Mirella Falconi The Hypoxia-Mimetic Agent Cobalt Chloride Differently Affects Human Mesenchymal Stem Cells in Their Chondrogenic Potential Stem Cells International |
| title | The Hypoxia-Mimetic Agent Cobalt Chloride Differently Affects Human Mesenchymal Stem Cells in Their Chondrogenic Potential |
| title_full | The Hypoxia-Mimetic Agent Cobalt Chloride Differently Affects Human Mesenchymal Stem Cells in Their Chondrogenic Potential |
| title_fullStr | The Hypoxia-Mimetic Agent Cobalt Chloride Differently Affects Human Mesenchymal Stem Cells in Their Chondrogenic Potential |
| title_full_unstemmed | The Hypoxia-Mimetic Agent Cobalt Chloride Differently Affects Human Mesenchymal Stem Cells in Their Chondrogenic Potential |
| title_short | The Hypoxia-Mimetic Agent Cobalt Chloride Differently Affects Human Mesenchymal Stem Cells in Their Chondrogenic Potential |
| title_sort | hypoxia mimetic agent cobalt chloride differently affects human mesenchymal stem cells in their chondrogenic potential |
| url | http://dx.doi.org/10.1155/2018/3237253 |
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