Morphometrical and Molecular Characterization of Oesophagostomum columbianum (Chabertiidae: Oesophagostominae) and Haemonchus contortus (Trichostrongylidae: Haemonchinae) Isolated from Goat (Capra hircus) in Sylhet, Bangladesh
This study was aimed at describing two (2) intestinal nematodes from naturally infected native breed of goats (Capra hircus) in Bangladesh, identified as Oesophagostomum columbianum (Curtice, 1890) Stossich 1899 and Haemonchus contortus (Rudolphi, 1803) Cobb, 1898. The identification was made based...
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2021-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/8863283 |
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| author | Tilak Chandra Nath Dongmin Lee Hansol Park Seongjun Choe Barakaeli Abdieli Ndosi Yeseul Kang Mohammed Mebarek Bia Chatanun Eamudomkarn Uday Kumar Mohanta Kazi Mehetazul Islam Jamal Uddin Bhuiyan Hyeong-Kyu Jeon Keeseon S. Eom |
| author_facet | Tilak Chandra Nath Dongmin Lee Hansol Park Seongjun Choe Barakaeli Abdieli Ndosi Yeseul Kang Mohammed Mebarek Bia Chatanun Eamudomkarn Uday Kumar Mohanta Kazi Mehetazul Islam Jamal Uddin Bhuiyan Hyeong-Kyu Jeon Keeseon S. Eom |
| author_sort | Tilak Chandra Nath |
| collection | DOAJ |
| description | This study was aimed at describing two (2) intestinal nematodes from naturally infected native breed of goats (Capra hircus) in Bangladesh, identified as Oesophagostomum columbianum (Curtice, 1890) Stossich 1899 and Haemonchus contortus (Rudolphi, 1803) Cobb, 1898. The identification was made based on morphometric features and was confirmed by amplifying internal transcribed spacer (ITS) and cytochrome c oxidase (cox1) gene. Well-developed lateral alae, distinct cervical papillae anteriorly to esophageal expansion, and male spicule length (0.73-0.79 mm, n=2) were characteristically observed in O. columbianum. At the same time, male spicule length (0.40-0.46 mm, n=2) and position of female vulvar flap (4.30-4.54 mm from posterior end, n=3) were observed in H. contortus. DNA sequence homology of the ITS and cox1 gene of both specimens revealed the same results, showing similarity to the GenBank sequences of O. columbianum (GenBank No. KC715827; JX188470) and H. contortus (GenBank No. KJ724377; HQ389229). Phylogenetic analysis computed by maximum livelihood (ML) from the ITS nucleotide sequences revealed that the O. columbianum and H. contortus isolates identified in this study were clustered in the same clade with isolates from China and Iran, respectively. This study, for the first time, illustrates the characteristics of O. columbianum and H. contortus in Bangladesh, combining both morphological and molecular data. The universal primer-based polymerase chain reaction (PCR) protocol could be an economical and efficient option for researchers from poor resource settings for precise identification of nematodes. The information generated in this study may contribute to formulating effective control strategies against these nematodes. |
| format | Article |
| id | doaj-art-5fe4b79842cf4162ab487fa4656b15bc |
| institution | OA Journals |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2021-01-01 |
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| series | Journal of Parasitology Research |
| spelling | doaj-art-5fe4b79842cf4162ab487fa4656b15bc2025-08-20T02:23:40ZengWileyJournal of Parasitology Research2090-00232090-00312021-01-01202110.1155/2021/88632838863283Morphometrical and Molecular Characterization of Oesophagostomum columbianum (Chabertiidae: Oesophagostominae) and Haemonchus contortus (Trichostrongylidae: Haemonchinae) Isolated from Goat (Capra hircus) in Sylhet, BangladeshTilak Chandra Nath0Dongmin Lee1Hansol Park2Seongjun Choe3Barakaeli Abdieli Ndosi4Yeseul Kang5Mohammed Mebarek Bia6Chatanun Eamudomkarn7Uday Kumar Mohanta8Kazi Mehetazul Islam9Jamal Uddin Bhuiyan10Hyeong-Kyu Jeon11Keeseon S. Eom12Department of Parasitology, Parasite Research Center and Parasite Resource Bank, School of Medicine, Chungbuk National University, Republic of KoreaDepartment of Parasitology, Parasite Research Center and Parasite Resource Bank, School of Medicine, Chungbuk National University, Republic of KoreaDepartment of Parasitology, Parasite Research Center and Parasite Resource Bank, School of Medicine, Chungbuk National University, Republic of KoreaDepartment of Parasitology, Parasite Research Center and Parasite Resource Bank, School of Medicine, Chungbuk National University, Republic of KoreaDepartment of Parasitology, Parasite Research Center and Parasite Resource Bank, School of Medicine, Chungbuk National University, Republic of KoreaDepartment of Parasitology, Parasite Research Center and Parasite Resource Bank, School of Medicine, Chungbuk National University, Republic of KoreaDepartment of Parasitology, Parasite Research Center and Parasite Resource Bank, School of Medicine, Chungbuk National University, Republic of KoreaDepartment of Parasitology, Parasite Research Center and Parasite Resource Bank, School of Medicine, Chungbuk National University, Republic of KoreaDepartment of Microbiology and Parasitology, Sher-e-Bangla Agricultural University, BangladeshDepartment of Parasitology, Sylhet Agricultural University, BangladeshDepartment of Parasitology, Sylhet Agricultural University, BangladeshDepartment of Parasitology, Parasite Research Center and Parasite Resource Bank, School of Medicine, Chungbuk National University, Republic of KoreaDepartment of Parasitology, Parasite Research Center and Parasite Resource Bank, School of Medicine, Chungbuk National University, Republic of KoreaThis study was aimed at describing two (2) intestinal nematodes from naturally infected native breed of goats (Capra hircus) in Bangladesh, identified as Oesophagostomum columbianum (Curtice, 1890) Stossich 1899 and Haemonchus contortus (Rudolphi, 1803) Cobb, 1898. The identification was made based on morphometric features and was confirmed by amplifying internal transcribed spacer (ITS) and cytochrome c oxidase (cox1) gene. Well-developed lateral alae, distinct cervical papillae anteriorly to esophageal expansion, and male spicule length (0.73-0.79 mm, n=2) were characteristically observed in O. columbianum. At the same time, male spicule length (0.40-0.46 mm, n=2) and position of female vulvar flap (4.30-4.54 mm from posterior end, n=3) were observed in H. contortus. DNA sequence homology of the ITS and cox1 gene of both specimens revealed the same results, showing similarity to the GenBank sequences of O. columbianum (GenBank No. KC715827; JX188470) and H. contortus (GenBank No. KJ724377; HQ389229). Phylogenetic analysis computed by maximum livelihood (ML) from the ITS nucleotide sequences revealed that the O. columbianum and H. contortus isolates identified in this study were clustered in the same clade with isolates from China and Iran, respectively. This study, for the first time, illustrates the characteristics of O. columbianum and H. contortus in Bangladesh, combining both morphological and molecular data. The universal primer-based polymerase chain reaction (PCR) protocol could be an economical and efficient option for researchers from poor resource settings for precise identification of nematodes. The information generated in this study may contribute to formulating effective control strategies against these nematodes.http://dx.doi.org/10.1155/2021/8863283 |
| spellingShingle | Tilak Chandra Nath Dongmin Lee Hansol Park Seongjun Choe Barakaeli Abdieli Ndosi Yeseul Kang Mohammed Mebarek Bia Chatanun Eamudomkarn Uday Kumar Mohanta Kazi Mehetazul Islam Jamal Uddin Bhuiyan Hyeong-Kyu Jeon Keeseon S. Eom Morphometrical and Molecular Characterization of Oesophagostomum columbianum (Chabertiidae: Oesophagostominae) and Haemonchus contortus (Trichostrongylidae: Haemonchinae) Isolated from Goat (Capra hircus) in Sylhet, Bangladesh Journal of Parasitology Research |
| title | Morphometrical and Molecular Characterization of Oesophagostomum columbianum (Chabertiidae: Oesophagostominae) and Haemonchus contortus (Trichostrongylidae: Haemonchinae) Isolated from Goat (Capra hircus) in Sylhet, Bangladesh |
| title_full | Morphometrical and Molecular Characterization of Oesophagostomum columbianum (Chabertiidae: Oesophagostominae) and Haemonchus contortus (Trichostrongylidae: Haemonchinae) Isolated from Goat (Capra hircus) in Sylhet, Bangladesh |
| title_fullStr | Morphometrical and Molecular Characterization of Oesophagostomum columbianum (Chabertiidae: Oesophagostominae) and Haemonchus contortus (Trichostrongylidae: Haemonchinae) Isolated from Goat (Capra hircus) in Sylhet, Bangladesh |
| title_full_unstemmed | Morphometrical and Molecular Characterization of Oesophagostomum columbianum (Chabertiidae: Oesophagostominae) and Haemonchus contortus (Trichostrongylidae: Haemonchinae) Isolated from Goat (Capra hircus) in Sylhet, Bangladesh |
| title_short | Morphometrical and Molecular Characterization of Oesophagostomum columbianum (Chabertiidae: Oesophagostominae) and Haemonchus contortus (Trichostrongylidae: Haemonchinae) Isolated from Goat (Capra hircus) in Sylhet, Bangladesh |
| title_sort | morphometrical and molecular characterization of oesophagostomum columbianum chabertiidae oesophagostominae and haemonchus contortus trichostrongylidae haemonchinae isolated from goat capra hircus in sylhet bangladesh |
| url | http://dx.doi.org/10.1155/2021/8863283 |
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