Hypoxia-Conditioned Mesenchymal Stem Cells (HC-MSC)-Derived Secretome Gel Induce IL-10 to Improve Diabetic Foot Ulcers via Reduced NF-κB p65 Gene Expression: A Randomized Controlled Trial
Background: Diabetic foot ulcers (DFUs) are a serious complication of diabetes mellitus, often leading to severe outcomes. Given the limitations of current treatments, other strategies are needed to address this gap. Recently, the administration of hypoxia-conditioned mesenchymal stem cells (HC-MSC)...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
International Medical Research and Development Corporation
2025-03-01
|
| Series: | International Journal of Biomedicine |
| Subjects: | |
| Online Access: | http://www.ijbm.org/articles/i57/ijbm_15(1)_oa4.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849762541933166592 |
|---|---|
| author | Nur Anna Chalimah Sadyah Heri-Nugroho Agung Putra Ignatius Riwanto |
| author_facet | Nur Anna Chalimah Sadyah Heri-Nugroho Agung Putra Ignatius Riwanto |
| author_sort | Nur Anna Chalimah Sadyah |
| collection | DOAJ |
| description | Background: Diabetic foot ulcers (DFUs) are a serious complication of diabetes mellitus, often leading to severe outcomes. Given the limitations of current treatments, other strategies are needed to address this gap. Recently, the administration of hypoxia-conditioned mesenchymal stem cells (HC-MSC) secretome has shown promise in treating DFUs, particularly in improving wound healing. This study aims to evaluate the efficacy of HC-MSC-derived secretome gel in treating DFUs by assessing its impact on inflammatory biomarkers, specifically IL-10 and NF-κB p65 gene expression.
Methods and Results: This randomized, prospective, controlled clinical trial included 16 patients with type 2 diabetes mellitus (T2DM) and Grade 2–3 DFUs. A control group (n=8) was treated with a placebo/base gel, and an intervention group was treated with HC-MSC-derived secretome gel produced by the Stem Cell and Cancer Research Center, Indonesia. The clinical wound volume, IL-10, and NF-κB p65 were assessed pre- and post-treatment.
The relative quantification (RQ) of IL-10 showed a significant increase in the intervention group on Day 7 after treatment compared to the placebo group (mean difference of 1.12, P<0.001), indicating an enhanced anti-inflammatory response. The RQ of NF-κB p65 gene expression significantly decreased in the intervention group (mean difference of 0.488, P=0.001), suggesting reduced inflammatory signaling. These results correlate with improved wound healing in the intervention group, evidenced by reductions in wound volume (mean difference of 0.826 cm3, P=0.002) on Day 7 after treatment.
Conclusion: HC-MSC-derived secretome gel significantly enhanced IL-10 expression and reduced NF-κB p65 gene expression in DFUs, improving wound healing outcomes. This approach shows promise as an alternative to conventional treatments. Further research is needed to understand the mechanisms involved. |
| format | Article |
| id | doaj-art-5fd45f044b7c41d6b85c4e6dfb1fbf05 |
| institution | DOAJ |
| issn | 2158-0510 2158-0529 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | International Medical Research and Development Corporation |
| record_format | Article |
| series | International Journal of Biomedicine |
| spelling | doaj-art-5fd45f044b7c41d6b85c4e6dfb1fbf052025-08-20T03:05:42ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292025-03-01151788310.21103/Article15(1)_OA4Hypoxia-Conditioned Mesenchymal Stem Cells (HC-MSC)-Derived Secretome Gel Induce IL-10 to Improve Diabetic Foot Ulcers via Reduced NF-κB p65 Gene Expression: A Randomized Controlled Trial Nur Anna Chalimah Sadyah0 Heri-Nugroho1Agung Putra2Ignatius Riwanto3Doctoral Study Program of Medical and Health Science, Universitas Diponegoro, Semarang, Indonesia; Department of Internal Medicine, Sultan Agung Islamic Hospital, Semarang, Indonesia; Department of Internal Medicine, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, IndonesiaDepartment of Internal Medicine, Faculty of Medicine, Universitas Diponegoro, Semarang, IndonesiaStem Cell and Cancer Research, Semarang, Indonesia; Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, IndonesiaDepartment of Surgery, Faculty of Medicine, Universitas Diponegoro, Semarang, IndonesiaBackground: Diabetic foot ulcers (DFUs) are a serious complication of diabetes mellitus, often leading to severe outcomes. Given the limitations of current treatments, other strategies are needed to address this gap. Recently, the administration of hypoxia-conditioned mesenchymal stem cells (HC-MSC) secretome has shown promise in treating DFUs, particularly in improving wound healing. This study aims to evaluate the efficacy of HC-MSC-derived secretome gel in treating DFUs by assessing its impact on inflammatory biomarkers, specifically IL-10 and NF-κB p65 gene expression. Methods and Results: This randomized, prospective, controlled clinical trial included 16 patients with type 2 diabetes mellitus (T2DM) and Grade 2–3 DFUs. A control group (n=8) was treated with a placebo/base gel, and an intervention group was treated with HC-MSC-derived secretome gel produced by the Stem Cell and Cancer Research Center, Indonesia. The clinical wound volume, IL-10, and NF-κB p65 were assessed pre- and post-treatment. The relative quantification (RQ) of IL-10 showed a significant increase in the intervention group on Day 7 after treatment compared to the placebo group (mean difference of 1.12, P<0.001), indicating an enhanced anti-inflammatory response. The RQ of NF-κB p65 gene expression significantly decreased in the intervention group (mean difference of 0.488, P=0.001), suggesting reduced inflammatory signaling. These results correlate with improved wound healing in the intervention group, evidenced by reductions in wound volume (mean difference of 0.826 cm3, P=0.002) on Day 7 after treatment. Conclusion: HC-MSC-derived secretome gel significantly enhanced IL-10 expression and reduced NF-κB p65 gene expression in DFUs, improving wound healing outcomes. This approach shows promise as an alternative to conventional treatments. Further research is needed to understand the mechanisms involved.http://www.ijbm.org/articles/i57/ijbm_15(1)_oa4.pdfdiabetes mellitusmesenchymal stem cellil-10p65wound healing |
| spellingShingle | Nur Anna Chalimah Sadyah Heri-Nugroho Agung Putra Ignatius Riwanto Hypoxia-Conditioned Mesenchymal Stem Cells (HC-MSC)-Derived Secretome Gel Induce IL-10 to Improve Diabetic Foot Ulcers via Reduced NF-κB p65 Gene Expression: A Randomized Controlled Trial International Journal of Biomedicine diabetes mellitus mesenchymal stem cell il-10 p65 wound healing |
| title | Hypoxia-Conditioned Mesenchymal Stem Cells (HC-MSC)-Derived Secretome Gel Induce IL-10 to Improve Diabetic Foot Ulcers via Reduced NF-κB p65 Gene Expression: A Randomized Controlled Trial |
| title_full | Hypoxia-Conditioned Mesenchymal Stem Cells (HC-MSC)-Derived Secretome Gel Induce IL-10 to Improve Diabetic Foot Ulcers via Reduced NF-κB p65 Gene Expression: A Randomized Controlled Trial |
| title_fullStr | Hypoxia-Conditioned Mesenchymal Stem Cells (HC-MSC)-Derived Secretome Gel Induce IL-10 to Improve Diabetic Foot Ulcers via Reduced NF-κB p65 Gene Expression: A Randomized Controlled Trial |
| title_full_unstemmed | Hypoxia-Conditioned Mesenchymal Stem Cells (HC-MSC)-Derived Secretome Gel Induce IL-10 to Improve Diabetic Foot Ulcers via Reduced NF-κB p65 Gene Expression: A Randomized Controlled Trial |
| title_short | Hypoxia-Conditioned Mesenchymal Stem Cells (HC-MSC)-Derived Secretome Gel Induce IL-10 to Improve Diabetic Foot Ulcers via Reduced NF-κB p65 Gene Expression: A Randomized Controlled Trial |
| title_sort | hypoxia conditioned mesenchymal stem cells hc msc derived secretome gel induce il 10 to improve diabetic foot ulcers via reduced nf κb p65 gene expression a randomized controlled trial |
| topic | diabetes mellitus mesenchymal stem cell il-10 p65 wound healing |
| url | http://www.ijbm.org/articles/i57/ijbm_15(1)_oa4.pdf |
| work_keys_str_mv | AT nurannachalimahsadyah hypoxiaconditionedmesenchymalstemcellshcmscderivedsecretomegelinduceil10toimprovediabeticfootulcersviareducednfkbp65geneexpressionarandomizedcontrolledtrial AT herinugroho hypoxiaconditionedmesenchymalstemcellshcmscderivedsecretomegelinduceil10toimprovediabeticfootulcersviareducednfkbp65geneexpressionarandomizedcontrolledtrial AT agungputra hypoxiaconditionedmesenchymalstemcellshcmscderivedsecretomegelinduceil10toimprovediabeticfootulcersviareducednfkbp65geneexpressionarandomizedcontrolledtrial AT ignatiusriwanto hypoxiaconditionedmesenchymalstemcellshcmscderivedsecretomegelinduceil10toimprovediabeticfootulcersviareducednfkbp65geneexpressionarandomizedcontrolledtrial |