Results of the 2023 rapid DNA multi-laboratory study – I-Chip

Identical sensitivity and mixture sample sets were provided to six external laboratories to evaluate the recent enhancements to the Rapid DNA technology for crime scene-type samples. Data were returned to the Federal Bureau of Investigation and the National Institute of Standards and Technology for...

Full description

Saved in:
Bibliographic Details
Main Authors: Erica L. Romsos, Lilliana I. Moreno, Douglas R. Hares
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Forensic Science International: Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2665910724000446
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850250231342432256
author Erica L. Romsos
Lilliana I. Moreno
Douglas R. Hares
author_facet Erica L. Romsos
Lilliana I. Moreno
Douglas R. Hares
author_sort Erica L. Romsos
collection DOAJ
description Identical sensitivity and mixture sample sets were provided to six external laboratories to evaluate the recent enhancements to the Rapid DNA technology for crime scene-type samples. Data were returned to the Federal Bureau of Investigation and the National Institute of Standards and Technology for review and analysis. Manual interpretation (modified Rapid DNA analysis) was conducted on all samples to assess the success of genotyping for both full profiles and the 20 Combined DNA Index System (CODIS) core STR loci using 18 total cellular DNA input amounts ranging from 102.4 ng to 0.025 ng. Two-person and three-person mixtures at varying ratios for two total DNA inputs were analyzed and observed allele percentages were compared to expected allele percentages.
format Article
id doaj-art-5fd349975b8e43e3beb587258ea7d3e0
institution OA Journals
issn 2665-9107
language English
publishDate 2024-12-01
publisher Elsevier
record_format Article
series Forensic Science International: Reports
spelling doaj-art-5fd349975b8e43e3beb587258ea7d3e02025-08-20T01:58:16ZengElsevierForensic Science International: Reports2665-91072024-12-011010039510.1016/j.fsir.2024.100395Results of the 2023 rapid DNA multi-laboratory study – I-ChipErica L. Romsos0Lilliana I. Moreno1Douglas R. Hares2Applied Genetics Group, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899-8314, USA; Corresponding author.FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USAFBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USAIdentical sensitivity and mixture sample sets were provided to six external laboratories to evaluate the recent enhancements to the Rapid DNA technology for crime scene-type samples. Data were returned to the Federal Bureau of Investigation and the National Institute of Standards and Technology for review and analysis. Manual interpretation (modified Rapid DNA analysis) was conducted on all samples to assess the success of genotyping for both full profiles and the 20 Combined DNA Index System (CODIS) core STR loci using 18 total cellular DNA input amounts ranging from 102.4 ng to 0.025 ng. Two-person and three-person mixtures at varying ratios for two total DNA inputs were analyzed and observed allele percentages were compared to expected allele percentages.http://www.sciencedirect.com/science/article/pii/S2665910724000446Rapid DNAModified rapid DNA analysisANDE 6 CFlexPlex
spellingShingle Erica L. Romsos
Lilliana I. Moreno
Douglas R. Hares
Results of the 2023 rapid DNA multi-laboratory study – I-Chip
Forensic Science International: Reports
Rapid DNA
Modified rapid DNA analysis
ANDE 6 C
FlexPlex
title Results of the 2023 rapid DNA multi-laboratory study – I-Chip
title_full Results of the 2023 rapid DNA multi-laboratory study – I-Chip
title_fullStr Results of the 2023 rapid DNA multi-laboratory study – I-Chip
title_full_unstemmed Results of the 2023 rapid DNA multi-laboratory study – I-Chip
title_short Results of the 2023 rapid DNA multi-laboratory study – I-Chip
title_sort results of the 2023 rapid dna multi laboratory study i chip
topic Rapid DNA
Modified rapid DNA analysis
ANDE 6 C
FlexPlex
url http://www.sciencedirect.com/science/article/pii/S2665910724000446
work_keys_str_mv AT ericalromsos resultsofthe2023rapiddnamultilaboratorystudyichip
AT lillianaimoreno resultsofthe2023rapiddnamultilaboratorystudyichip
AT douglasrhares resultsofthe2023rapiddnamultilaboratorystudyichip