Estrogen deficiency influences SEC23A gene expression in the odontogenic region of incisors – a murine model study
Recent scientific evidence suggests a close relationship between estrogen deficiency and vitamin D- related genes. Estrogen and vitamin D were involved with alterations in odontogenesis and tooth eruption process. Objective: The aim of the present study was to evaluate the influence of estrogen def...
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| Language: | English |
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Universidade Estadual Paulista
2024-03-01
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| Series: | Brazilian Dental Science |
| Online Access: | https://ojs.ict.unesp.br/index.php/cob/article/view/3827 |
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| author | Clara Costa do Nascimento Marcus Augusto Silveira Millena Cristina de Paula Martinelle Ferreira da Rocha Taranto Agnes Schröder Christian Kirschneck Paulo Nelson-Filho César Penazzo Lepri Flares Baratto-Filho Erika Calvano Küchler Isabela Madalena |
| author_facet | Clara Costa do Nascimento Marcus Augusto Silveira Millena Cristina de Paula Martinelle Ferreira da Rocha Taranto Agnes Schröder Christian Kirschneck Paulo Nelson-Filho César Penazzo Lepri Flares Baratto-Filho Erika Calvano Küchler Isabela Madalena |
| author_sort | Clara Costa do Nascimento |
| collection | DOAJ |
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Recent scientific evidence suggests a close relationship between estrogen deficiency and vitamin D- related genes. Estrogen and vitamin D were involved with alterations in odontogenesis and tooth eruption process. Objective: The aim of the present study was to evaluate the influence of estrogen deficiency on the expression of genes related to the activation and degradation of vitamin D in the odontogenic region of incisors in a murine model. Material and Methods: This is an experimental clinical study that used female Wistar Hannover rats. The animals were randomly divided into two groups according to the intervention received: Hypoestrogenism Group – animals submitted to estrogen deficiency by ovariectomy surgery and Control Group – animals submitted to sham surgery. Surgical intervention was performed in the prepubertal period; the animals were followed throughout the pubertal period. After euthanasia, the hemimandibles were removed to evaluate the mRNA expression of the vitamin D-related genes AMDHD1, CYP24A1, NADSYN1 and SEC23A in the odontogenic region of incisors through real time PCR. Student’s t test was used to compare means. Kruskal-Wallis test and Dunn’s posttest were also used. The level of significance was 5%. Results: SEC23A was overexpressed in the estrogen deficiency condition in the odontogenic region (p=0.021). Conclusion: Estrogen deficiency may influence the expression of the SEC23A gene involved in the activation and degradation of vitamin D in the odontogenic region of incisors in a murine model.
KEYWORDS
Estrogen; 25-hydroxyvitamin D3; Gene expression; Odontogenesis; Vitamin D.
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| format | Article |
| id | doaj-art-5fd23ac0095f40a9be97286fe5cf9e1d |
| institution | Kabale University |
| issn | 2178-6011 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Universidade Estadual Paulista |
| record_format | Article |
| series | Brazilian Dental Science |
| spelling | doaj-art-5fd23ac0095f40a9be97286fe5cf9e1d2025-08-22T14:49:21ZengUniversidade Estadual PaulistaBrazilian Dental Science2178-60112024-03-0127110.4322/bds.2024.e3827Estrogen deficiency influences SEC23A gene expression in the odontogenic region of incisors – a murine model studyClara Costa do Nascimento 0https://orcid.org/0009-0007-9461-8475Marcus Augusto Silveira 1https://orcid.org/0009-0002-4287-952XMillena Cristina de Paula 2https://orcid.org/0009-0005-9023-0874Martinelle Ferreira da Rocha Taranto 3https://orcid.org/0000-0001-5556-4506Agnes Schröder4https://orcid.org/0000-0001-6010-9831Christian Kirschneck 5https://orcid.org/0000-0001-9473-8724Paulo Nelson-Filho 6https://orcid.org/0000-0001-8802-6480César Penazzo Lepri 7https://orcid.org/0000-0003-4372-9718Flares Baratto-Filho 8https://orcid.org/0000-0002-5649-7234Erika Calvano Küchler 9https://orcid.org/0000-0001-5351-2526Isabela Madalena10https://orcid.org/0000-0002-4486-1318Centro Universitário Presidente Tancredo de Almeida Neves, Faculdade de Odontologia. São João del Rei, MG, Brazil.Centro Universitário Presidente Tancredo de Almeida Neves, Faculdade de Odontologia. São João del Rei, MG, Brazil.Centro Universitário Presidente Tancredo de Almeida Neves, Faculdade de Odontologia. São João del Rei, MG, Brazil.Centro Universitário Presidente Tancredo de Almeida Neves, Faculdade de Odontologia. São João del Rei, MG, Brazil.University of Regensburg, Department of Orthodontics. Regensburg, Germany.University of Regensburg, Department of Orthodontics. Regensburg, Germany.Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Clínica Infantil. Ribeirão Preto, SP, Brazil.Universidade de Uberaba, Departamento de Biomateriais. Uberaba, MG, Brazil.Universidade Tuiuti do Paraná, Faculdade de Odontologia. Curitiba, PR, Brazil.Universidade de Uberaba, Departamento de Biomateriais. Uberaba, MG, Brazil.Centro Universitário Presidente Tancredo de Almeida Neves, Faculdade de Odontologia. São João del Rei, MG, Brazil. Recent scientific evidence suggests a close relationship between estrogen deficiency and vitamin D- related genes. Estrogen and vitamin D were involved with alterations in odontogenesis and tooth eruption process. Objective: The aim of the present study was to evaluate the influence of estrogen deficiency on the expression of genes related to the activation and degradation of vitamin D in the odontogenic region of incisors in a murine model. Material and Methods: This is an experimental clinical study that used female Wistar Hannover rats. The animals were randomly divided into two groups according to the intervention received: Hypoestrogenism Group – animals submitted to estrogen deficiency by ovariectomy surgery and Control Group – animals submitted to sham surgery. Surgical intervention was performed in the prepubertal period; the animals were followed throughout the pubertal period. After euthanasia, the hemimandibles were removed to evaluate the mRNA expression of the vitamin D-related genes AMDHD1, CYP24A1, NADSYN1 and SEC23A in the odontogenic region of incisors through real time PCR. Student’s t test was used to compare means. Kruskal-Wallis test and Dunn’s posttest were also used. The level of significance was 5%. Results: SEC23A was overexpressed in the estrogen deficiency condition in the odontogenic region (p=0.021). Conclusion: Estrogen deficiency may influence the expression of the SEC23A gene involved in the activation and degradation of vitamin D in the odontogenic region of incisors in a murine model. KEYWORDS Estrogen; 25-hydroxyvitamin D3; Gene expression; Odontogenesis; Vitamin D. https://ojs.ict.unesp.br/index.php/cob/article/view/3827 |
| spellingShingle | Clara Costa do Nascimento Marcus Augusto Silveira Millena Cristina de Paula Martinelle Ferreira da Rocha Taranto Agnes Schröder Christian Kirschneck Paulo Nelson-Filho César Penazzo Lepri Flares Baratto-Filho Erika Calvano Küchler Isabela Madalena Estrogen deficiency influences SEC23A gene expression in the odontogenic region of incisors – a murine model study Brazilian Dental Science |
| title | Estrogen deficiency influences SEC23A gene expression in the odontogenic region of incisors – a murine model study |
| title_full | Estrogen deficiency influences SEC23A gene expression in the odontogenic region of incisors – a murine model study |
| title_fullStr | Estrogen deficiency influences SEC23A gene expression in the odontogenic region of incisors – a murine model study |
| title_full_unstemmed | Estrogen deficiency influences SEC23A gene expression in the odontogenic region of incisors – a murine model study |
| title_short | Estrogen deficiency influences SEC23A gene expression in the odontogenic region of incisors – a murine model study |
| title_sort | estrogen deficiency influences sec23a gene expression in the odontogenic region of incisors a murine model study |
| url | https://ojs.ict.unesp.br/index.php/cob/article/view/3827 |
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