Comparative transcriptomic rhythms in the mouse and human prefrontal cortex
IntroductionAlterations in multiple subregions of the human prefrontal cortex (PFC) have been heavily implicated in psychiatric diseases. Moreover, emerging evidence suggests that circadian rhythms in gene expression are present across the brain, including in the PFC, and that these rhythms are alte...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2024.1524615/full |
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| author | Jennifer N. Burns Jennifer N. Burns Aaron K. Jenkins Xiangning Xue Kaitlyn A. Petersen Kaitlyn A. Petersen Kyle D. Ketchesin Kyle D. Ketchesin Megan S. Perez Megan S. Perez Chelsea A. Vadnie Madeline R. Scott Madeline R. Scott Marianne L. Seney Marianne L. Seney George C. Tseng Colleen A. McClung Colleen A. McClung |
| author_facet | Jennifer N. Burns Jennifer N. Burns Aaron K. Jenkins Xiangning Xue Kaitlyn A. Petersen Kaitlyn A. Petersen Kyle D. Ketchesin Kyle D. Ketchesin Megan S. Perez Megan S. Perez Chelsea A. Vadnie Madeline R. Scott Madeline R. Scott Marianne L. Seney Marianne L. Seney George C. Tseng Colleen A. McClung Colleen A. McClung |
| author_sort | Jennifer N. Burns |
| collection | DOAJ |
| description | IntroductionAlterations in multiple subregions of the human prefrontal cortex (PFC) have been heavily implicated in psychiatric diseases. Moreover, emerging evidence suggests that circadian rhythms in gene expression are present across the brain, including in the PFC, and that these rhythms are altered in disease. However, investigation into the potential circadian mechanisms underlying these diseases in animal models must contend with the fact that the human PFC is highly evolved and specialized relative to that of rodents.MethodsHere, we use RNA sequencing to lay the groundwork for translational studies of molecular rhythms through a sex-specific, cross species comparison of transcriptomic rhythms between the mouse medial PFC (mPFC) and two subregions of the human PFC, the anterior cingulate cortex (ACC) and the dorsolateral PFC (DLPFC).ResultsWe find that while circadian rhythm signaling is conserved across species and subregions, there is a phase shift in the expression of core clock genes between the mouse mPFC and human PFC subregions that differs by sex. Furthermore, we find that the identity of rhythmic transcripts is largely unique between the mouse mPFC and human PFC subregions, with the most overlap (20%, 236 transcripts) between the mouse mPFC and the human ACC in females. Nevertheless, we find that basic biological processes are enriched for rhythmic transcripts across species, with key differences between regions and sexes.DiscussionTogether, this work highlights both the evolutionary conservation of transcriptomic rhythms and the advancement of the human PFC, underscoring the importance of considering cross-species differences when using animal models. |
| format | Article |
| id | doaj-art-5fcc1eb2c140495080071f056cbf32e6 |
| institution | Kabale University |
| issn | 1662-453X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neuroscience |
| spelling | doaj-art-5fcc1eb2c140495080071f056cbf32e62025-01-13T06:10:46ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2025-01-011810.3389/fnins.2024.15246151524615Comparative transcriptomic rhythms in the mouse and human prefrontal cortexJennifer N. Burns0Jennifer N. Burns1Aaron K. Jenkins2Xiangning Xue3Kaitlyn A. Petersen4Kaitlyn A. Petersen5Kyle D. Ketchesin6Kyle D. Ketchesin7Megan S. Perez8Megan S. Perez9Chelsea A. Vadnie10Madeline R. Scott11Madeline R. Scott12Marianne L. Seney13Marianne L. Seney14George C. Tseng15Colleen A. McClung16Colleen A. McClung17Translational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesCenter for Neuroscience, University of Pittsburgh, Pittsburgh, PA, United StatesTranslational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesDepartment of Biostatistics, University of Pittsburgh, Pittsburgh, PA, United StatesTranslational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesCenter for Neuroscience, University of Pittsburgh, Pittsburgh, PA, United StatesTranslational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesCenter for Neuroscience, University of Pittsburgh, Pittsburgh, PA, United StatesTranslational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesDepartment of Human Genetics, School of Public Health, University of Pittsburgh, Pittsburgh, PA, United StatesDavid O. Robbins Neuroscience Program, Department of Psychology, Ohio Wesleyan University, Delaware, OH, United StatesTranslational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesCenter for Neuroscience, University of Pittsburgh, Pittsburgh, PA, United StatesTranslational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesCenter for Neuroscience, University of Pittsburgh, Pittsburgh, PA, United StatesDepartment of Biostatistics, University of Pittsburgh, Pittsburgh, PA, United StatesTranslational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesCenter for Neuroscience, University of Pittsburgh, Pittsburgh, PA, United StatesIntroductionAlterations in multiple subregions of the human prefrontal cortex (PFC) have been heavily implicated in psychiatric diseases. Moreover, emerging evidence suggests that circadian rhythms in gene expression are present across the brain, including in the PFC, and that these rhythms are altered in disease. However, investigation into the potential circadian mechanisms underlying these diseases in animal models must contend with the fact that the human PFC is highly evolved and specialized relative to that of rodents.MethodsHere, we use RNA sequencing to lay the groundwork for translational studies of molecular rhythms through a sex-specific, cross species comparison of transcriptomic rhythms between the mouse medial PFC (mPFC) and two subregions of the human PFC, the anterior cingulate cortex (ACC) and the dorsolateral PFC (DLPFC).ResultsWe find that while circadian rhythm signaling is conserved across species and subregions, there is a phase shift in the expression of core clock genes between the mouse mPFC and human PFC subregions that differs by sex. Furthermore, we find that the identity of rhythmic transcripts is largely unique between the mouse mPFC and human PFC subregions, with the most overlap (20%, 236 transcripts) between the mouse mPFC and the human ACC in females. Nevertheless, we find that basic biological processes are enriched for rhythmic transcripts across species, with key differences between regions and sexes.DiscussionTogether, this work highlights both the evolutionary conservation of transcriptomic rhythms and the advancement of the human PFC, underscoring the importance of considering cross-species differences when using animal models.https://www.frontiersin.org/articles/10.3389/fnins.2024.1524615/fullcircadian rhythmstranscriptomicsprefrontal cortexmousehuman post mortem tissue |
| spellingShingle | Jennifer N. Burns Jennifer N. Burns Aaron K. Jenkins Xiangning Xue Kaitlyn A. Petersen Kaitlyn A. Petersen Kyle D. Ketchesin Kyle D. Ketchesin Megan S. Perez Megan S. Perez Chelsea A. Vadnie Madeline R. Scott Madeline R. Scott Marianne L. Seney Marianne L. Seney George C. Tseng Colleen A. McClung Colleen A. McClung Comparative transcriptomic rhythms in the mouse and human prefrontal cortex Frontiers in Neuroscience circadian rhythms transcriptomics prefrontal cortex mouse human post mortem tissue |
| title | Comparative transcriptomic rhythms in the mouse and human prefrontal cortex |
| title_full | Comparative transcriptomic rhythms in the mouse and human prefrontal cortex |
| title_fullStr | Comparative transcriptomic rhythms in the mouse and human prefrontal cortex |
| title_full_unstemmed | Comparative transcriptomic rhythms in the mouse and human prefrontal cortex |
| title_short | Comparative transcriptomic rhythms in the mouse and human prefrontal cortex |
| title_sort | comparative transcriptomic rhythms in the mouse and human prefrontal cortex |
| topic | circadian rhythms transcriptomics prefrontal cortex mouse human post mortem tissue |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2024.1524615/full |
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