Sarcoidosis and Non-small Cell Lung Cancer: Expression of miR-145, miR-301, and miR-449
Background: In the field, sarcoidosis is a condition characterized by inflammation, with an unknown origin often impacting the lungs and chest lymph nodes. On the other hand, lung cancer stands out as a fatal form of cancer affecting both men and women. It is challenging to distinguish sarcoidosis f...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Biomedical and Biotechnology Research Journal |
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| Online Access: | https://journals.lww.com/10.4103/bbrj.bbrj_349_24 |
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| author | Negin Khosravi Abdolreza Mohamadnia Changiz Ahmadi Zadeh Naghmeh Bahram Saeid Ghorbian |
| author_facet | Negin Khosravi Abdolreza Mohamadnia Changiz Ahmadi Zadeh Naghmeh Bahram Saeid Ghorbian |
| author_sort | Negin Khosravi |
| collection | DOAJ |
| description | Background:
In the field, sarcoidosis is a condition characterized by inflammation, with an unknown origin often impacting the lungs and chest lymph nodes. On the other hand, lung cancer stands out as a fatal form of cancer affecting both men and women. It is challenging to distinguish sarcoidosis from lung cancer. A method to diagnose and treat diseases involves examining the biomarkers associated with the condition. This research aimed to analyze the levels of miR-449, miR-301, and miR-145 in lymph node tissue of individuals with sarcoidosis compared to a control group using the real-time polymerase chain reaction (PCR) and compare their expression with non-small cell lung cancer tissue.
Methods:
In this study, we gathered the tissue samples from the lymph nodes of individuals with sarcoidosis, cancer patients, and controls. Subsequently, we conducted real-time PCR analysis to assess the levels of miR-301, miR-145, and miR-449 gene expression in the samples. We also evaluated the variation in the biomarker expression levels and their correlation between genes and rock graphs.
Results:
The levels of miR-301 expression were found to be elevated in lymph node samples from patients with sarcoidosis and in cancer tissue compared to those from healthy individuals. In addition, miR-449 expression was higher in lymph node samples from sarcoidosis patients than in healthy individuals and it was lower in cancer tissue samples. Furthermore, there were variations in the expression levels of miR-145, between lymph node tissue of sarcoidosis patients and cancer tissue.
Conclusion:
The investigated biomarkers are suitable for the prognosis and treatment of sarcoidosis and lung cancer and can be used in faster and more accurate diagnosis of the disease and as a result more effective treatment. |
| format | Article |
| id | doaj-art-5fc303e5142b4aa4a5d3cbfbe7792928 |
| institution | Kabale University |
| issn | 2588-9834 2588-9842 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Biomedical and Biotechnology Research Journal |
| spelling | doaj-art-5fc303e5142b4aa4a5d3cbfbe77929282025-01-08T09:53:05ZengWolters Kluwer Medknow PublicationsBiomedical and Biotechnology Research Journal2588-98342588-98422024-12-018451752310.4103/bbrj.bbrj_349_24Sarcoidosis and Non-small Cell Lung Cancer: Expression of miR-145, miR-301, and miR-449Negin KhosraviAbdolreza MohamadniaChangiz Ahmadi ZadehNaghmeh BahramSaeid GhorbianBackground: In the field, sarcoidosis is a condition characterized by inflammation, with an unknown origin often impacting the lungs and chest lymph nodes. On the other hand, lung cancer stands out as a fatal form of cancer affecting both men and women. It is challenging to distinguish sarcoidosis from lung cancer. A method to diagnose and treat diseases involves examining the biomarkers associated with the condition. This research aimed to analyze the levels of miR-449, miR-301, and miR-145 in lymph node tissue of individuals with sarcoidosis compared to a control group using the real-time polymerase chain reaction (PCR) and compare their expression with non-small cell lung cancer tissue. Methods: In this study, we gathered the tissue samples from the lymph nodes of individuals with sarcoidosis, cancer patients, and controls. Subsequently, we conducted real-time PCR analysis to assess the levels of miR-301, miR-145, and miR-449 gene expression in the samples. We also evaluated the variation in the biomarker expression levels and their correlation between genes and rock graphs. Results: The levels of miR-301 expression were found to be elevated in lymph node samples from patients with sarcoidosis and in cancer tissue compared to those from healthy individuals. In addition, miR-449 expression was higher in lymph node samples from sarcoidosis patients than in healthy individuals and it was lower in cancer tissue samples. Furthermore, there were variations in the expression levels of miR-145, between lymph node tissue of sarcoidosis patients and cancer tissue. Conclusion: The investigated biomarkers are suitable for the prognosis and treatment of sarcoidosis and lung cancer and can be used in faster and more accurate diagnosis of the disease and as a result more effective treatment.https://journals.lww.com/10.4103/bbrj.bbrj_349_24mir-145mir-301mir-449non-small cell lung cancersarcoidosis |
| spellingShingle | Negin Khosravi Abdolreza Mohamadnia Changiz Ahmadi Zadeh Naghmeh Bahram Saeid Ghorbian Sarcoidosis and Non-small Cell Lung Cancer: Expression of miR-145, miR-301, and miR-449 Biomedical and Biotechnology Research Journal mir-145 mir-301 mir-449 non-small cell lung cancer sarcoidosis |
| title | Sarcoidosis and Non-small Cell Lung Cancer: Expression of miR-145, miR-301, and miR-449 |
| title_full | Sarcoidosis and Non-small Cell Lung Cancer: Expression of miR-145, miR-301, and miR-449 |
| title_fullStr | Sarcoidosis and Non-small Cell Lung Cancer: Expression of miR-145, miR-301, and miR-449 |
| title_full_unstemmed | Sarcoidosis and Non-small Cell Lung Cancer: Expression of miR-145, miR-301, and miR-449 |
| title_short | Sarcoidosis and Non-small Cell Lung Cancer: Expression of miR-145, miR-301, and miR-449 |
| title_sort | sarcoidosis and non small cell lung cancer expression of mir 145 mir 301 and mir 449 |
| topic | mir-145 mir-301 mir-449 non-small cell lung cancer sarcoidosis |
| url | https://journals.lww.com/10.4103/bbrj.bbrj_349_24 |
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