Comparative study on the in vitro effects of Pseudomonas aeruginosa and seaweed alginates on human gut microbiota.
Alginates pertain to organic polysaccharides that have been extensively used in food- and medicine-related industries. The present study obtained alginates from an alginate overproducing Pseudomonas aeruginosa PAO1 mutant by screening transposon mutagenesis libraries. The interaction between bacteri...
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171576&type=printable |
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| author | Shaofeng Bai Huahai Chen Liying Zhu Wei Liu Hongwei D Yu Xin Wang Yeshi Yin |
| author_facet | Shaofeng Bai Huahai Chen Liying Zhu Wei Liu Hongwei D Yu Xin Wang Yeshi Yin |
| author_sort | Shaofeng Bai |
| collection | DOAJ |
| description | Alginates pertain to organic polysaccharides that have been extensively used in food- and medicine-related industries. The present study obtained alginates from an alginate overproducing Pseudomonas aeruginosa PAO1 mutant by screening transposon mutagenesis libraries. The interaction between bacterial and seaweed alginates and gut microbiota were further studied by using an in vitro batch fermentation system. Thin-layer chromatography (TLC) analysis indicated that both bacterial and seaweed alginates can be completely degraded by fecal bacteria isolated from study volunteers, indicating that a minor structural difference between bacterial and seaweed alginates (O-acetylation and lack of G-G blocks) didn't affect the digestion of alginates by human microbiota. Although, the digestion of bacterial and seaweed alginates was attributed to different Bacteroides xylanisolvens strains, they harbored similar alginate lyase genes. Genus Bacteroides with alginate-degrading capability were enriched in growth medium containing bacterial or seaweed alginates after in vitro fermentation. Short-chain fatty acid (SCFA) production in both bacterial and seaweed alginates was also comparable, but was significantly higher than the same medium using starch. In summary, the present study has isolated an alginate-overproducing P. aeruginosa mutant strain. Both seaweed and bacterial alginates were degraded by human gut microbiota, and their regulatory function on gut microbiota was similar. |
| format | Article |
| id | doaj-art-5fb7df5dfe244def8dcd593af8853768 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-5fb7df5dfe244def8dcd593af88537682025-08-20T02:45:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017157610.1371/journal.pone.0171576Comparative study on the in vitro effects of Pseudomonas aeruginosa and seaweed alginates on human gut microbiota.Shaofeng BaiHuahai ChenLiying ZhuWei LiuHongwei D YuXin WangYeshi YinAlginates pertain to organic polysaccharides that have been extensively used in food- and medicine-related industries. The present study obtained alginates from an alginate overproducing Pseudomonas aeruginosa PAO1 mutant by screening transposon mutagenesis libraries. The interaction between bacterial and seaweed alginates and gut microbiota were further studied by using an in vitro batch fermentation system. Thin-layer chromatography (TLC) analysis indicated that both bacterial and seaweed alginates can be completely degraded by fecal bacteria isolated from study volunteers, indicating that a minor structural difference between bacterial and seaweed alginates (O-acetylation and lack of G-G blocks) didn't affect the digestion of alginates by human microbiota. Although, the digestion of bacterial and seaweed alginates was attributed to different Bacteroides xylanisolvens strains, they harbored similar alginate lyase genes. Genus Bacteroides with alginate-degrading capability were enriched in growth medium containing bacterial or seaweed alginates after in vitro fermentation. Short-chain fatty acid (SCFA) production in both bacterial and seaweed alginates was also comparable, but was significantly higher than the same medium using starch. In summary, the present study has isolated an alginate-overproducing P. aeruginosa mutant strain. Both seaweed and bacterial alginates were degraded by human gut microbiota, and their regulatory function on gut microbiota was similar.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171576&type=printable |
| spellingShingle | Shaofeng Bai Huahai Chen Liying Zhu Wei Liu Hongwei D Yu Xin Wang Yeshi Yin Comparative study on the in vitro effects of Pseudomonas aeruginosa and seaweed alginates on human gut microbiota. PLoS ONE |
| title | Comparative study on the in vitro effects of Pseudomonas aeruginosa and seaweed alginates on human gut microbiota. |
| title_full | Comparative study on the in vitro effects of Pseudomonas aeruginosa and seaweed alginates on human gut microbiota. |
| title_fullStr | Comparative study on the in vitro effects of Pseudomonas aeruginosa and seaweed alginates on human gut microbiota. |
| title_full_unstemmed | Comparative study on the in vitro effects of Pseudomonas aeruginosa and seaweed alginates on human gut microbiota. |
| title_short | Comparative study on the in vitro effects of Pseudomonas aeruginosa and seaweed alginates on human gut microbiota. |
| title_sort | comparative study on the in vitro effects of pseudomonas aeruginosa and seaweed alginates on human gut microbiota |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171576&type=printable |
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