Stimulation of glucose uptake by edible Pholiota nameko (T.Itô) S.Ito & S.Imai extract counteracts palmitate-induced insulin resistance in C2C12 myotubes
Pholiota nameko (P. nameko) was identified as an edible mushroom known for its abundance of bioactive compounds. Previous studies suggested that extracts from this mushroom possess antioxidant properties attributed to the presence of phenolic acids, such as 4-hydroxybenzoic acid (4-HBA) and cinnamic...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2025.1520875/full |
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| author | Seng-Kai Vong Chiao-Ming Chen Arpita Das Lie-Fen Shyur Yu-Ming Ju Huei-Mei Hsieh Shu-Hui Yang Viola Varga Sing-Chung Li |
| author_facet | Seng-Kai Vong Chiao-Ming Chen Arpita Das Lie-Fen Shyur Yu-Ming Ju Huei-Mei Hsieh Shu-Hui Yang Viola Varga Sing-Chung Li |
| author_sort | Seng-Kai Vong |
| collection | DOAJ |
| description | Pholiota nameko (P. nameko) was identified as an edible mushroom known for its abundance of bioactive compounds. Previous studies suggested that extracts from this mushroom possess antioxidant properties attributed to the presence of phenolic acids, such as 4-hydroxybenzoic acid (4-HBA) and cinnamic acid (CA). These compounds show promise in potentially ameliorating insulin resistance. In this study, the highest levels of 4-HBA and CA were selected from six P. nameko extracts using ultra-high-pressure liquid chromatography (UHPLC) to investigate whether they could improve the glucose uptake resistance induced by palmitate in C2C12 myotubes. Additionally, the protein expression of GLUT4 was examined. The results indicated that P. nameko hot water extract (PN-HW) exhibited the highest total polyphenol content (9.34 ± 0.31 mg GAE/g) and demonstrates strong free radical scavenging ability (77.4 ± 1.1%). Furthermore, PN-HW contains notable amounts of 4-HBA (4.02 ± 0.17 mg/g DW) and CA (2.01 ± 0.09 mg/g DW). Importantly, the cell viability reveals that PN-HW extracts did not have a toxic effect on C2C12 myotubes. PN-HW significantly enhances glucose uptake at doses of 25 μg/mL. Hence, it was suggested that PN-HW might contain elevated levels of phenolic acids, potentially mitigating impaired glucose uptake caused by high-fat content and consequently enhancing the glucose uptake capacity of C2C12 cells. |
| format | Article |
| id | doaj-art-5fa993d2af4747468bc87c83df645d60 |
| institution | DOAJ |
| issn | 2571-581X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainable Food Systems |
| spelling | doaj-art-5fa993d2af4747468bc87c83df645d602025-08-20T02:43:49ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2025-02-01910.3389/fsufs.2025.15208751520875Stimulation of glucose uptake by edible Pholiota nameko (T.Itô) S.Ito & S.Imai extract counteracts palmitate-induced insulin resistance in C2C12 myotubesSeng-Kai Vong0Chiao-Ming Chen1Arpita Das2Lie-Fen Shyur3Yu-Ming Ju4Huei-Mei Hsieh5Shu-Hui Yang6Viola Varga7Sing-Chung Li8Department of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, TaiwanDepartment of Food Science, Nutrition and Nutraceutical Biotechnology, Shih Chien University, Taipei, TaiwanDepartment of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, TaiwanAgricultural Biotechnology Research Center, Academia Sinica, Taipei, TaiwanInstitute of Plant and Microbial Biology, Academia Sinica, Taipei, TaiwanInstitute of Plant and Microbial Biology, Academia Sinica, Taipei, TaiwanFengshan Tropical Horticultural Experiment Branch, Taiwan Agricultural Research Institute, Kaohsiung, TaiwanInstitute of Translational Medicine, Semmelweis University, Budapest, HungaryDepartment of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, TaiwanPholiota nameko (P. nameko) was identified as an edible mushroom known for its abundance of bioactive compounds. Previous studies suggested that extracts from this mushroom possess antioxidant properties attributed to the presence of phenolic acids, such as 4-hydroxybenzoic acid (4-HBA) and cinnamic acid (CA). These compounds show promise in potentially ameliorating insulin resistance. In this study, the highest levels of 4-HBA and CA were selected from six P. nameko extracts using ultra-high-pressure liquid chromatography (UHPLC) to investigate whether they could improve the glucose uptake resistance induced by palmitate in C2C12 myotubes. Additionally, the protein expression of GLUT4 was examined. The results indicated that P. nameko hot water extract (PN-HW) exhibited the highest total polyphenol content (9.34 ± 0.31 mg GAE/g) and demonstrates strong free radical scavenging ability (77.4 ± 1.1%). Furthermore, PN-HW contains notable amounts of 4-HBA (4.02 ± 0.17 mg/g DW) and CA (2.01 ± 0.09 mg/g DW). Importantly, the cell viability reveals that PN-HW extracts did not have a toxic effect on C2C12 myotubes. PN-HW significantly enhances glucose uptake at doses of 25 μg/mL. Hence, it was suggested that PN-HW might contain elevated levels of phenolic acids, potentially mitigating impaired glucose uptake caused by high-fat content and consequently enhancing the glucose uptake capacity of C2C12 cells.https://www.frontiersin.org/articles/10.3389/fsufs.2025.1520875/fullPholiota nameko water extractinsulin resistancephenolic acidscinnamic acid4-hydroxybenzoic acid |
| spellingShingle | Seng-Kai Vong Chiao-Ming Chen Arpita Das Lie-Fen Shyur Yu-Ming Ju Huei-Mei Hsieh Shu-Hui Yang Viola Varga Sing-Chung Li Stimulation of glucose uptake by edible Pholiota nameko (T.Itô) S.Ito & S.Imai extract counteracts palmitate-induced insulin resistance in C2C12 myotubes Frontiers in Sustainable Food Systems Pholiota nameko water extract insulin resistance phenolic acids cinnamic acid 4-hydroxybenzoic acid |
| title | Stimulation of glucose uptake by edible Pholiota nameko (T.Itô) S.Ito & S.Imai extract counteracts palmitate-induced insulin resistance in C2C12 myotubes |
| title_full | Stimulation of glucose uptake by edible Pholiota nameko (T.Itô) S.Ito & S.Imai extract counteracts palmitate-induced insulin resistance in C2C12 myotubes |
| title_fullStr | Stimulation of glucose uptake by edible Pholiota nameko (T.Itô) S.Ito & S.Imai extract counteracts palmitate-induced insulin resistance in C2C12 myotubes |
| title_full_unstemmed | Stimulation of glucose uptake by edible Pholiota nameko (T.Itô) S.Ito & S.Imai extract counteracts palmitate-induced insulin resistance in C2C12 myotubes |
| title_short | Stimulation of glucose uptake by edible Pholiota nameko (T.Itô) S.Ito & S.Imai extract counteracts palmitate-induced insulin resistance in C2C12 myotubes |
| title_sort | stimulation of glucose uptake by edible pholiota nameko t ito s ito s imai extract counteracts palmitate induced insulin resistance in c2c12 myotubes |
| topic | Pholiota nameko water extract insulin resistance phenolic acids cinnamic acid 4-hydroxybenzoic acid |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2025.1520875/full |
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