CRISPR/Cas9-Mediated Knockout of <i>BmGDAP2</i> in the Silkworm, <i>Bombyx mori</i>: Extended Lifespan and Altered Gene Expression Impacting Developmental Pathways
Ganglioside-induced differentiation-associated protein 2 (<i>GDAP2</i>) is a gene involved in hereditary cerebellar ataxia. At present, little is known about the function of <i>GDAP2</i> in insects. In this study, <i>BmGDAP2</i> was detected to be highly expressed...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/16/4/354 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850143772049932288 |
|---|---|
| author | Chaojun Yuan Zichong Zhou Qifeng Guo Ying Yang Yue Sun Yong Liu Wenyi Jia Shuoqi Fan Jinxin Wu Xiaoting Hua Ping Lin Ping Zhao Qingyou Xia |
| author_facet | Chaojun Yuan Zichong Zhou Qifeng Guo Ying Yang Yue Sun Yong Liu Wenyi Jia Shuoqi Fan Jinxin Wu Xiaoting Hua Ping Lin Ping Zhao Qingyou Xia |
| author_sort | Chaojun Yuan |
| collection | DOAJ |
| description | Ganglioside-induced differentiation-associated protein 2 (<i>GDAP2</i>) is a gene involved in hereditary cerebellar ataxia. At present, little is known about the function of <i>GDAP2</i> in insects. In this study, <i>BmGDAP2</i> was detected to be highly expressed in the head, epidermis, midgut, and anterior silk glands of silkworms. We generated a knockout mutant, <i>BmGDAP2</i> (<i>BmGDAP2</i><sup>KO</sup>), using the CRISPR/Cas9 system. Compared with that of the wild-type, the growth cycle of <i>BmGDAP2</i><sup>KO</sup> larvae was significantly prolonged, while their body size was reduced. Furthermore, we found 149 differentially expressed genes (DEGs) between <i>BmGDAP2</i><sup>KO</sup> and the wild-type, including 106 upregulated and 43 downregulated genes. GO annotation analysis indicated that <i>BmGDAP2</i> primarily influences structural and molecular activities, as well as catalytic and binding functions. KEGG pathway analysis revealed that the differentially expressed genes were mainly enriched in pathways related to peroxidase activity, hormone synthesis, apoptosis, and longevity regulation. Further investigation focused on candidate genes related to these pathways. We found that the expression levels of <i>MAD2L1</i>, which can inhibit cell proliferation and promote apoptosis, and <i>Aurka</i>-b, which plays a crucial role in cell cycle regulation, were significantly reduced in <i>BmGDAP2</i><sup>KO</sup> silkworms. These changes may interfere with the normal functions of cell division, leading to the prolonged developmental cycle observed in <i>BmGDAP2</i><sup>KO</sup> larvae. Our findings demonstrate that knockout of <i>BmGDAP2</i> significantly prolongs the life cycle of <i>Bombyx mori</i> by affecting genes related to autophagy, apoptosis, and hormone regulation. |
| format | Article |
| id | doaj-art-5fa7708311ae4ff4a3daa64e8932ae68 |
| institution | OA Journals |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-5fa7708311ae4ff4a3daa64e8932ae682025-08-20T02:28:36ZengMDPI AGInsects2075-44502025-03-0116435410.3390/insects16040354CRISPR/Cas9-Mediated Knockout of <i>BmGDAP2</i> in the Silkworm, <i>Bombyx mori</i>: Extended Lifespan and Altered Gene Expression Impacting Developmental PathwaysChaojun Yuan0Zichong Zhou1Qifeng Guo2Ying Yang3Yue Sun4Yong Liu5Wenyi Jia6Shuoqi Fan7Jinxin Wu8Xiaoting Hua9Ping Lin10Ping Zhao11Qingyou Xia12Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaGanglioside-induced differentiation-associated protein 2 (<i>GDAP2</i>) is a gene involved in hereditary cerebellar ataxia. At present, little is known about the function of <i>GDAP2</i> in insects. In this study, <i>BmGDAP2</i> was detected to be highly expressed in the head, epidermis, midgut, and anterior silk glands of silkworms. We generated a knockout mutant, <i>BmGDAP2</i> (<i>BmGDAP2</i><sup>KO</sup>), using the CRISPR/Cas9 system. Compared with that of the wild-type, the growth cycle of <i>BmGDAP2</i><sup>KO</sup> larvae was significantly prolonged, while their body size was reduced. Furthermore, we found 149 differentially expressed genes (DEGs) between <i>BmGDAP2</i><sup>KO</sup> and the wild-type, including 106 upregulated and 43 downregulated genes. GO annotation analysis indicated that <i>BmGDAP2</i> primarily influences structural and molecular activities, as well as catalytic and binding functions. KEGG pathway analysis revealed that the differentially expressed genes were mainly enriched in pathways related to peroxidase activity, hormone synthesis, apoptosis, and longevity regulation. Further investigation focused on candidate genes related to these pathways. We found that the expression levels of <i>MAD2L1</i>, which can inhibit cell proliferation and promote apoptosis, and <i>Aurka</i>-b, which plays a crucial role in cell cycle regulation, were significantly reduced in <i>BmGDAP2</i><sup>KO</sup> silkworms. These changes may interfere with the normal functions of cell division, leading to the prolonged developmental cycle observed in <i>BmGDAP2</i><sup>KO</sup> larvae. Our findings demonstrate that knockout of <i>BmGDAP2</i> significantly prolongs the life cycle of <i>Bombyx mori</i> by affecting genes related to autophagy, apoptosis, and hormone regulation.https://www.mdpi.com/2075-4450/16/4/354<i>GDAP2</i>silkwormdevelopmentlifespan |
| spellingShingle | Chaojun Yuan Zichong Zhou Qifeng Guo Ying Yang Yue Sun Yong Liu Wenyi Jia Shuoqi Fan Jinxin Wu Xiaoting Hua Ping Lin Ping Zhao Qingyou Xia CRISPR/Cas9-Mediated Knockout of <i>BmGDAP2</i> in the Silkworm, <i>Bombyx mori</i>: Extended Lifespan and Altered Gene Expression Impacting Developmental Pathways Insects <i>GDAP2</i> silkworm development lifespan |
| title | CRISPR/Cas9-Mediated Knockout of <i>BmGDAP2</i> in the Silkworm, <i>Bombyx mori</i>: Extended Lifespan and Altered Gene Expression Impacting Developmental Pathways |
| title_full | CRISPR/Cas9-Mediated Knockout of <i>BmGDAP2</i> in the Silkworm, <i>Bombyx mori</i>: Extended Lifespan and Altered Gene Expression Impacting Developmental Pathways |
| title_fullStr | CRISPR/Cas9-Mediated Knockout of <i>BmGDAP2</i> in the Silkworm, <i>Bombyx mori</i>: Extended Lifespan and Altered Gene Expression Impacting Developmental Pathways |
| title_full_unstemmed | CRISPR/Cas9-Mediated Knockout of <i>BmGDAP2</i> in the Silkworm, <i>Bombyx mori</i>: Extended Lifespan and Altered Gene Expression Impacting Developmental Pathways |
| title_short | CRISPR/Cas9-Mediated Knockout of <i>BmGDAP2</i> in the Silkworm, <i>Bombyx mori</i>: Extended Lifespan and Altered Gene Expression Impacting Developmental Pathways |
| title_sort | crispr cas9 mediated knockout of i bmgdap2 i in the silkworm i bombyx mori i extended lifespan and altered gene expression impacting developmental pathways |
| topic | <i>GDAP2</i> silkworm development lifespan |
| url | https://www.mdpi.com/2075-4450/16/4/354 |
| work_keys_str_mv | AT chaojunyuan crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT zichongzhou crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT qifengguo crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT yingyang crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT yuesun crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT yongliu crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT wenyijia crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT shuoqifan crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT jinxinwu crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT xiaotinghua crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT pinglin crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT pingzhao crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways AT qingyouxia crisprcas9mediatedknockoutofibmgdap2iinthesilkwormibombyxmoriiextendedlifespanandalteredgeneexpressionimpactingdevelopmentalpathways |