Synthesis of Microgel Sulfated Carboxymethyl Cellulose (SCMC) by Double Crosslinked Alginate from Lindur Fruit (Bruguiera gymnorrhiza) for Diabetic Ulcer Healing

Microgels have been recognized as promising scaffolds for diabetic wound care due to their favorable properties in promoting wound healing. This study aims to synthesize a microgel using sulfated carboxymethyl cellulose (SCMC) derived from Lindur fruit (Bruguiera gymnorrhiza) for potential applicati...

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Main Authors: Jobin Armando Hutapea, Nesfitra Dzakwan Zaki, Silvina Faradilla Syahrum, Surya Andika Putra, Sarah Aknesia Marbun, Indra Lasmana Tarigan
Format: Article
Language:English
Published: Universitas Syiah Kuala, Chemical Engineering Department 2025-05-01
Series:Jurnal Rekayasa Kimia & Lingkungan
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Online Access:https://jurnal.usk.ac.id/RKL/article/view/40112
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author Jobin Armando Hutapea
Nesfitra Dzakwan Zaki
Silvina Faradilla Syahrum
Surya Andika Putra
Sarah Aknesia Marbun
Indra Lasmana Tarigan
author_facet Jobin Armando Hutapea
Nesfitra Dzakwan Zaki
Silvina Faradilla Syahrum
Surya Andika Putra
Sarah Aknesia Marbun
Indra Lasmana Tarigan
author_sort Jobin Armando Hutapea
collection DOAJ
description Microgels have been recognized as promising scaffolds for diabetic wound care due to their favorable properties in promoting wound healing. This study aims to synthesize a microgel using sulfated carboxymethyl cellulose (SCMC) derived from Lindur fruit (Bruguiera gymnorrhiza) for potential application in diabetic ulcer treatment. The synthesis was conducted through a double-crosslinking reaction, and the resulting microgel was characterized using infrared (IR) spectroscopy, scanning electron microscopy (SEM), and in vivo analysis in male mice (Mus musculus). The results demonstrated that the yield of Ca-alginate isolate ranged from 18.67% to 19.46%, with a purity of 19.5%–33.1%, viscosity of 31.19–31.46 cP, and stable pH at 7. The Ca-alginate isolate exhibited antioxidant activity at 50 ppm with an inhibition percentage of 22.3%. The modification of SCMC was successfully achieved, as confirmed by the presence of characteristic absorption peaks at 1220 cm⁻¹ (asymmetric S–O stretching) and 870 cm⁻¹ (symmetric C–O–S stretching vibrations), indicating successful sulfate group incorporation. The microgel swelling ratio was recorded at 660% for microgel A, 700% for microgel B, and 720% for microgel C, with microgel C exhibiting the highest swelling capacity. In vivo wound healing assessment revealed that microgel C facilitated more rapid wound closure, with wound sizes reducing progressively from 0.9 cm on day 3 to 0.5 cm on day 5, 0.3 cm on day 7, and 0.1 cm on day 14. These findings suggest that SCMC-based microgel with alginate derived from Lindur fruit holds significant potential as an innovative formulation for diabetic wound management
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spelling doaj-art-5fa496dddaff41c9b82746239f6cbf602025-08-20T03:27:25ZengUniversitas Syiah Kuala, Chemical Engineering DepartmentJurnal Rekayasa Kimia & Lingkungan1412-50642356-16612025-05-01201465610.23955/rkl.v20i1.4011220468Synthesis of Microgel Sulfated Carboxymethyl Cellulose (SCMC) by Double Crosslinked Alginate from Lindur Fruit (Bruguiera gymnorrhiza) for Diabetic Ulcer HealingJobin Armando Hutapea0Nesfitra Dzakwan Zaki1Silvina Faradilla Syahrum2Surya Andika Putra3Sarah Aknesia Marbun4Indra Lasmana Tarigan5Department of Chemistry, Faculty of Science and Technology, Universitas JambiDepartment of Chemistry, Faculty of Science and Technology, Universitas JambiDepartement of Chemistry, Faculty of Science and Technology, Universitas JambiDepartment of Chemistry, Faculty of Science and Technology, Universitas JambiDepartment of Chemistry, Faculty of Science and Technology, Universitas JambiDepartment of Chemistry, Faculty of Science and Technology, Universitas JambiMicrogels have been recognized as promising scaffolds for diabetic wound care due to their favorable properties in promoting wound healing. This study aims to synthesize a microgel using sulfated carboxymethyl cellulose (SCMC) derived from Lindur fruit (Bruguiera gymnorrhiza) for potential application in diabetic ulcer treatment. The synthesis was conducted through a double-crosslinking reaction, and the resulting microgel was characterized using infrared (IR) spectroscopy, scanning electron microscopy (SEM), and in vivo analysis in male mice (Mus musculus). The results demonstrated that the yield of Ca-alginate isolate ranged from 18.67% to 19.46%, with a purity of 19.5%–33.1%, viscosity of 31.19–31.46 cP, and stable pH at 7. The Ca-alginate isolate exhibited antioxidant activity at 50 ppm with an inhibition percentage of 22.3%. The modification of SCMC was successfully achieved, as confirmed by the presence of characteristic absorption peaks at 1220 cm⁻¹ (asymmetric S–O stretching) and 870 cm⁻¹ (symmetric C–O–S stretching vibrations), indicating successful sulfate group incorporation. The microgel swelling ratio was recorded at 660% for microgel A, 700% for microgel B, and 720% for microgel C, with microgel C exhibiting the highest swelling capacity. In vivo wound healing assessment revealed that microgel C facilitated more rapid wound closure, with wound sizes reducing progressively from 0.9 cm on day 3 to 0.5 cm on day 5, 0.3 cm on day 7, and 0.1 cm on day 14. These findings suggest that SCMC-based microgel with alginate derived from Lindur fruit holds significant potential as an innovative formulation for diabetic wound managementhttps://jurnal.usk.ac.id/RKL/article/view/40112diabetesmicrogelscmc
spellingShingle Jobin Armando Hutapea
Nesfitra Dzakwan Zaki
Silvina Faradilla Syahrum
Surya Andika Putra
Sarah Aknesia Marbun
Indra Lasmana Tarigan
Synthesis of Microgel Sulfated Carboxymethyl Cellulose (SCMC) by Double Crosslinked Alginate from Lindur Fruit (Bruguiera gymnorrhiza) for Diabetic Ulcer Healing
Jurnal Rekayasa Kimia & Lingkungan
diabetes
microgel
scmc
title Synthesis of Microgel Sulfated Carboxymethyl Cellulose (SCMC) by Double Crosslinked Alginate from Lindur Fruit (Bruguiera gymnorrhiza) for Diabetic Ulcer Healing
title_full Synthesis of Microgel Sulfated Carboxymethyl Cellulose (SCMC) by Double Crosslinked Alginate from Lindur Fruit (Bruguiera gymnorrhiza) for Diabetic Ulcer Healing
title_fullStr Synthesis of Microgel Sulfated Carboxymethyl Cellulose (SCMC) by Double Crosslinked Alginate from Lindur Fruit (Bruguiera gymnorrhiza) for Diabetic Ulcer Healing
title_full_unstemmed Synthesis of Microgel Sulfated Carboxymethyl Cellulose (SCMC) by Double Crosslinked Alginate from Lindur Fruit (Bruguiera gymnorrhiza) for Diabetic Ulcer Healing
title_short Synthesis of Microgel Sulfated Carboxymethyl Cellulose (SCMC) by Double Crosslinked Alginate from Lindur Fruit (Bruguiera gymnorrhiza) for Diabetic Ulcer Healing
title_sort synthesis of microgel sulfated carboxymethyl cellulose scmc by double crosslinked alginate from lindur fruit bruguiera gymnorrhiza for diabetic ulcer healing
topic diabetes
microgel
scmc
url https://jurnal.usk.ac.id/RKL/article/view/40112
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