Molybdenum regulates phosphorus cycling species diversity and improves soil phosphorus availability through key flavonoids in the soybean (Glycine max)
Applying molybdenum (Mo) fertilizer can improve soil phosphorus (P) bioavailability, reduce the need for P fertilizers in agriculture, and enhance crop growth. However, the precise mechanisms behind these benefits are not yet fully understood. For the first time, we demonstrate the impact of Mo appl...
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Elsevier
2025-04-01
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| Series: | Geoderma |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0016706125000801 |
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| author | Xiaoming Qin Yining Liu Qingyun Xu Chengxiao Hu Songwei Wu Xuecheng Sun Qiling Tan |
| author_facet | Xiaoming Qin Yining Liu Qingyun Xu Chengxiao Hu Songwei Wu Xuecheng Sun Qiling Tan |
| author_sort | Xiaoming Qin |
| collection | DOAJ |
| description | Applying molybdenum (Mo) fertilizer can improve soil phosphorus (P) bioavailability, reduce the need for P fertilizers in agriculture, and enhance crop growth. However, the precise mechanisms behind these benefits are not yet fully understood. For the first time, we demonstrate the impact of Mo application on the transformation of P forms, metabolites, and microorganisms in the soybean rhizosphere. We carried out a series of pot experiments under controlled conditions, applying varying levels of Mo and collecting samples from the soybean rhizosphere across different treatments to analyze P forms, metabolic profiles, and microbial communities comprehensively. Mo application enhanced soybean P uptake and growth by promoted the conversion of aluminum-bound P (Al-P) and organic P to available P. The underlying mechanism involves the regulatory effect of Mo on the abundance of metabolites in the soil, thereby reshaping the structure of the rhizosphere microbial community. Two key Mo-mediated flavonoids, chrysin (Cs) and phlorizin (Pz), significantly promoted soybean growth and P absorption. Subsequently, Soil metagenomics and phosphate-solubilizing bacteria (PSB) addition experiments confirmed that these flavonoids increased P cycling genes (e.g., gcd and phoD) and microorganisms, facilitating stable P transformation into labile P, and aiding PSB (Bacillus subtilis) in further enhancing soil P availability. In summary, we have demonstrated for the first time that trace metals regulate the abundance of soil P cycling microorganisms by influencing crop-secreted flavonoids. This ultimately improves soil P bioavailability, providing a new insight for sustainable agricultural development. |
| format | Article |
| id | doaj-art-5fa0576289fe4e3d96eb813e228b8cb2 |
| institution | DOAJ |
| issn | 1872-6259 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
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| series | Geoderma |
| spelling | doaj-art-5fa0576289fe4e3d96eb813e228b8cb22025-08-20T03:08:35ZengElsevierGeoderma1872-62592025-04-0145611724210.1016/j.geoderma.2025.117242Molybdenum regulates phosphorus cycling species diversity and improves soil phosphorus availability through key flavonoids in the soybean (Glycine max)Xiaoming Qin0Yining Liu1Qingyun Xu2Chengxiao Hu3Songwei Wu4Xuecheng Sun5Qiling Tan6Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Micro-elements Research Center, College of Resource and Environment, Huazhong Agricultural University, Wuhan 430070, China; Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 430064, ChinaKey Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Micro-elements Research Center, College of Resource and Environment, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Micro-elements Research Center, College of Resource and Environment, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Micro-elements Research Center, College of Resource and Environment, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Micro-elements Research Center, College of Resource and Environment, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Micro-elements Research Center, College of Resource and Environment, Huazhong Agricultural University, Wuhan 430070, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Wuhan 430070, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, ChinaKey Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Micro-elements Research Center, College of Resource and Environment, Huazhong Agricultural University, Wuhan 430070, China; Corresponding author.Applying molybdenum (Mo) fertilizer can improve soil phosphorus (P) bioavailability, reduce the need for P fertilizers in agriculture, and enhance crop growth. However, the precise mechanisms behind these benefits are not yet fully understood. For the first time, we demonstrate the impact of Mo application on the transformation of P forms, metabolites, and microorganisms in the soybean rhizosphere. We carried out a series of pot experiments under controlled conditions, applying varying levels of Mo and collecting samples from the soybean rhizosphere across different treatments to analyze P forms, metabolic profiles, and microbial communities comprehensively. Mo application enhanced soybean P uptake and growth by promoted the conversion of aluminum-bound P (Al-P) and organic P to available P. The underlying mechanism involves the regulatory effect of Mo on the abundance of metabolites in the soil, thereby reshaping the structure of the rhizosphere microbial community. Two key Mo-mediated flavonoids, chrysin (Cs) and phlorizin (Pz), significantly promoted soybean growth and P absorption. Subsequently, Soil metagenomics and phosphate-solubilizing bacteria (PSB) addition experiments confirmed that these flavonoids increased P cycling genes (e.g., gcd and phoD) and microorganisms, facilitating stable P transformation into labile P, and aiding PSB (Bacillus subtilis) in further enhancing soil P availability. In summary, we have demonstrated for the first time that trace metals regulate the abundance of soil P cycling microorganisms by influencing crop-secreted flavonoids. This ultimately improves soil P bioavailability, providing a new insight for sustainable agricultural development.http://www.sciencedirect.com/science/article/pii/S0016706125000801Molybdenum fertilizerPhosphorus bioavailabilitySoybean rhizosphereFlavonoidsSustainable agriculture |
| spellingShingle | Xiaoming Qin Yining Liu Qingyun Xu Chengxiao Hu Songwei Wu Xuecheng Sun Qiling Tan Molybdenum regulates phosphorus cycling species diversity and improves soil phosphorus availability through key flavonoids in the soybean (Glycine max) Geoderma Molybdenum fertilizer Phosphorus bioavailability Soybean rhizosphere Flavonoids Sustainable agriculture |
| title | Molybdenum regulates phosphorus cycling species diversity and improves soil phosphorus availability through key flavonoids in the soybean (Glycine max) |
| title_full | Molybdenum regulates phosphorus cycling species diversity and improves soil phosphorus availability through key flavonoids in the soybean (Glycine max) |
| title_fullStr | Molybdenum regulates phosphorus cycling species diversity and improves soil phosphorus availability through key flavonoids in the soybean (Glycine max) |
| title_full_unstemmed | Molybdenum regulates phosphorus cycling species diversity and improves soil phosphorus availability through key flavonoids in the soybean (Glycine max) |
| title_short | Molybdenum regulates phosphorus cycling species diversity and improves soil phosphorus availability through key flavonoids in the soybean (Glycine max) |
| title_sort | molybdenum regulates phosphorus cycling species diversity and improves soil phosphorus availability through key flavonoids in the soybean glycine max |
| topic | Molybdenum fertilizer Phosphorus bioavailability Soybean rhizosphere Flavonoids Sustainable agriculture |
| url | http://www.sciencedirect.com/science/article/pii/S0016706125000801 |
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