Analysis of physiological, biochemical mechanism, and pigment related genes in leaf and petal coloration of Paeonia qiui
In addition to flower color, the red new leaves of tree peonies in spring are also ornamental, with the leaf color of Paeonia qiui being the most typical. This study aimed to explore the formation mechanism of P. qiui leaf and flower color by quantitative evaluation and correlating the factors affec...
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| Language: | English |
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Maximum Academic Press
2025-01-01
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| Series: | Ornamental Plant Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/opr-0025-0011 |
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| author | Shanrong Dong Shuiyan Yu Zihan Yang Yingan Niu Xinyue Liu Chenxi Wei Jianrang Luo |
| author_facet | Shanrong Dong Shuiyan Yu Zihan Yang Yingan Niu Xinyue Liu Chenxi Wei Jianrang Luo |
| author_sort | Shanrong Dong |
| collection | DOAJ |
| description | In addition to flower color, the red new leaves of tree peonies in spring are also ornamental, with the leaf color of Paeonia qiui being the most typical. This study aimed to explore the formation mechanism of P. qiui leaf and flower color by quantitative evaluation and correlating the factors affecting the coloration of leaves and petals. Leaves from eight developmental stages, and petals from five developmental stages of P. qiui were collected for determination of color parameter, cellular pH, anthocyanin, anthoxanthin, chlorophyll, and carotenoid contents, and it was found that anthocyanins and chlorophylls were closely related to leaf coloration, with anthocyanins being the determinant of leaf red color, while anthocyanidins and anthoxanthins affect the petal coloration, with anthocyanins being more influential. High-performance liquid chromatography (HPLC) analysis showed that leaves contain peonidin-3,5-di-O-glucoside (Pn3G5G), peonidin-3-O-glucoside (Pn3G), and cyanidin-3,5-di-O-glucoside (Cy3G5G), and petals contain Pn3G5G, Pn3G, Cy3G5G, and pelargonidin-3,5-di-O-glucoside (Pg3G5G), with Pn3G5G being the most abundant in both leaves and petals. qRT-PCR analysis revealed that PqDFR, PqANS, and PqMYB113 significantly and positively correlated with anthocyanin accumulation in leaves, and PqF3H promoted anthocyanin accumulation to a greater extent in petals. The findings of this study can provide a theoretical reference for the cultivation of new tree peony varieties and germplasm innovation. |
| format | Article |
| id | doaj-art-5f9ff20e2e774cd086ebea82b15b8a76 |
| institution | DOAJ |
| issn | 2769-2094 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Ornamental Plant Research |
| spelling | doaj-art-5f9ff20e2e774cd086ebea82b15b8a762025-08-20T03:18:35ZengMaximum Academic PressOrnamental Plant Research2769-20942025-01-015111010.48130/opr-0025-0011opr-0025-0011Analysis of physiological, biochemical mechanism, and pigment related genes in leaf and petal coloration of Paeonia qiuiShanrong Dong0Shuiyan Yu1Zihan Yang2Yingan Niu3Xinyue Liu4Chenxi Wei5Jianrang Luo6College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, Shaanxi, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, Shaanxi, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, Shaanxi, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, Shaanxi, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, Shaanxi, ChinaCollege of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, Shaanxi, ChinaIn addition to flower color, the red new leaves of tree peonies in spring are also ornamental, with the leaf color of Paeonia qiui being the most typical. This study aimed to explore the formation mechanism of P. qiui leaf and flower color by quantitative evaluation and correlating the factors affecting the coloration of leaves and petals. Leaves from eight developmental stages, and petals from five developmental stages of P. qiui were collected for determination of color parameter, cellular pH, anthocyanin, anthoxanthin, chlorophyll, and carotenoid contents, and it was found that anthocyanins and chlorophylls were closely related to leaf coloration, with anthocyanins being the determinant of leaf red color, while anthocyanidins and anthoxanthins affect the petal coloration, with anthocyanins being more influential. High-performance liquid chromatography (HPLC) analysis showed that leaves contain peonidin-3,5-di-O-glucoside (Pn3G5G), peonidin-3-O-glucoside (Pn3G), and cyanidin-3,5-di-O-glucoside (Cy3G5G), and petals contain Pn3G5G, Pn3G, Cy3G5G, and pelargonidin-3,5-di-O-glucoside (Pg3G5G), with Pn3G5G being the most abundant in both leaves and petals. qRT-PCR analysis revealed that PqDFR, PqANS, and PqMYB113 significantly and positively correlated with anthocyanin accumulation in leaves, and PqF3H promoted anthocyanin accumulation to a greater extent in petals. The findings of this study can provide a theoretical reference for the cultivation of new tree peony varieties and germplasm innovation.https://www.maxapress.com/article/doi/10.48130/opr-0025-0011paeonia qiuileafpetalcolorationanthocyaninsbiosynthetic genes |
| spellingShingle | Shanrong Dong Shuiyan Yu Zihan Yang Yingan Niu Xinyue Liu Chenxi Wei Jianrang Luo Analysis of physiological, biochemical mechanism, and pigment related genes in leaf and petal coloration of Paeonia qiui Ornamental Plant Research paeonia qiui leaf petal coloration anthocyanins biosynthetic genes |
| title | Analysis of physiological, biochemical mechanism, and pigment related genes in leaf and petal coloration of Paeonia qiui |
| title_full | Analysis of physiological, biochemical mechanism, and pigment related genes in leaf and petal coloration of Paeonia qiui |
| title_fullStr | Analysis of physiological, biochemical mechanism, and pigment related genes in leaf and petal coloration of Paeonia qiui |
| title_full_unstemmed | Analysis of physiological, biochemical mechanism, and pigment related genes in leaf and petal coloration of Paeonia qiui |
| title_short | Analysis of physiological, biochemical mechanism, and pigment related genes in leaf and petal coloration of Paeonia qiui |
| title_sort | analysis of physiological biochemical mechanism and pigment related genes in leaf and petal coloration of paeonia qiui |
| topic | paeonia qiui leaf petal coloration anthocyanins biosynthetic genes |
| url | https://www.maxapress.com/article/doi/10.48130/opr-0025-0011 |
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