Antioxidant and Cytotoxic Potential of Local Endemic Plant Pastinaca zozimoides Fenzl
P. zozimoides is local endemic in Nigde province, Turkey. There has been no previous examination of the chemical composition and bioactivity of that plant extract. In this study, we studied total phenolic content, antioxidant properties and cytotoxic effect of methanolic extracts of plant sample. Ac...
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Hasan Eleroğlu
2021-04-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3715 |
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| author | Cihan Düşgün Teoman Kankılıç Cemil İşlek Dilara Fatma Balı Özgür Kankılıç |
| author_facet | Cihan Düşgün Teoman Kankılıç Cemil İşlek Dilara Fatma Balı Özgür Kankılıç |
| author_sort | Cihan Düşgün |
| collection | DOAJ |
| description | P. zozimoides is local endemic in Nigde province, Turkey. There has been no previous examination of the chemical composition and bioactivity of that plant extract. In this study, we studied total phenolic content, antioxidant properties and cytotoxic effect of methanolic extracts of plant sample. According to the Folin-Ciocalteau method, the total phenolics of the extracts were determined spectrophometric. The antioxidant activity was conducted DPPH (1,1’-diphenyl-2-picrylhydrazyl) radical scavenging method and free radical reducing power assay. The cytotoxic effect was studied using MTT assay cell viability on cancer cell lines as well as on Caco-2 cell lines. The total phenolic content of P. zozimoides extract was found 28.79±0.68 μg GAE /mg. The free radical scavenger activities of the DPPH was 31.69%±1.61, 85.15%±0.13, 86.96%±0.085 for 1, 5 and 10 mg/mL, respectively. The free radical reducing power assay was 0.375±0.52, 1.587±0.71, 1.798±0.84 for 1, 5 and 10 mg/mL compared to ascorbic acid standard. On Caco-2 cell lines, the extract of plant showed no cytotoxic potential. Because of its phenolic constituents and its antioxidant capacity it can be considered a healthy nutrient. |
| format | Article |
| id | doaj-art-5f9a628d2d834ef29682ce682383da67 |
| institution | DOAJ |
| issn | 2148-127X |
| language | English |
| publishDate | 2021-04-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-5f9a628d2d834ef29682ce682383da672025-08-20T03:19:35ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2021-04-019464664910.24925/turjaf.v9i4.646-649.37152037Antioxidant and Cytotoxic Potential of Local Endemic Plant Pastinaca zozimoides FenzlCihan Düşgün0Teoman Kankılıç1Cemil İşlek2Dilara Fatma Balı3Özgür Kankılıç4Faculty of Science Literature, Department of Biotechnology, Niğde Ömer Halisdemir University, 51240 NiğdeFaculty of Science Literature, Department of Biotechnology, Niğde Ömer Halisdemir University, 51240 NiğdeFaculty of Science Literature, Department of Biotechnology, Niğde Ömer Halisdemir University, 51240 NiğdeFaculty of Medicine, Medical Biology, Niğde Ömer Halisdemir University, 51240 NiğdeFaculty of Science Literature, Department of Biotechnology, Niğde Ömer Halisdemir University, 51240 NiğdeP. zozimoides is local endemic in Nigde province, Turkey. There has been no previous examination of the chemical composition and bioactivity of that plant extract. In this study, we studied total phenolic content, antioxidant properties and cytotoxic effect of methanolic extracts of plant sample. According to the Folin-Ciocalteau method, the total phenolics of the extracts were determined spectrophometric. The antioxidant activity was conducted DPPH (1,1’-diphenyl-2-picrylhydrazyl) radical scavenging method and free radical reducing power assay. The cytotoxic effect was studied using MTT assay cell viability on cancer cell lines as well as on Caco-2 cell lines. The total phenolic content of P. zozimoides extract was found 28.79±0.68 μg GAE /mg. The free radical scavenger activities of the DPPH was 31.69%±1.61, 85.15%±0.13, 86.96%±0.085 for 1, 5 and 10 mg/mL, respectively. The free radical reducing power assay was 0.375±0.52, 1.587±0.71, 1.798±0.84 for 1, 5 and 10 mg/mL compared to ascorbic acid standard. On Caco-2 cell lines, the extract of plant showed no cytotoxic potential. Because of its phenolic constituents and its antioxidant capacity it can be considered a healthy nutrient.http://www.agrifoodscience.com/index.php/TURJAF/article/view/3715total phenolicantioxidantanticarcinogenicpastinaca zozimoideslocal endemic |
| spellingShingle | Cihan Düşgün Teoman Kankılıç Cemil İşlek Dilara Fatma Balı Özgür Kankılıç Antioxidant and Cytotoxic Potential of Local Endemic Plant Pastinaca zozimoides Fenzl Turkish Journal of Agriculture: Food Science and Technology total phenolic antioxidant anticarcinogenic pastinaca zozimoides local endemic |
| title | Antioxidant and Cytotoxic Potential of Local Endemic Plant Pastinaca zozimoides Fenzl |
| title_full | Antioxidant and Cytotoxic Potential of Local Endemic Plant Pastinaca zozimoides Fenzl |
| title_fullStr | Antioxidant and Cytotoxic Potential of Local Endemic Plant Pastinaca zozimoides Fenzl |
| title_full_unstemmed | Antioxidant and Cytotoxic Potential of Local Endemic Plant Pastinaca zozimoides Fenzl |
| title_short | Antioxidant and Cytotoxic Potential of Local Endemic Plant Pastinaca zozimoides Fenzl |
| title_sort | antioxidant and cytotoxic potential of local endemic plant pastinaca zozimoides fenzl |
| topic | total phenolic antioxidant anticarcinogenic pastinaca zozimoides local endemic |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3715 |
| work_keys_str_mv | AT cihandusgun antioxidantandcytotoxicpotentialoflocalendemicplantpastinacazozimoidesfenzl AT teomankankılıc antioxidantandcytotoxicpotentialoflocalendemicplantpastinacazozimoidesfenzl AT cemilislek antioxidantandcytotoxicpotentialoflocalendemicplantpastinacazozimoidesfenzl AT dilarafatmabalı antioxidantandcytotoxicpotentialoflocalendemicplantpastinacazozimoidesfenzl AT ozgurkankılıc antioxidantandcytotoxicpotentialoflocalendemicplantpastinacazozimoidesfenzl |