Neonatal gut microbiota succession in mice mapped over time, site, injury and single immunoglobulin interleukin-1 related receptor genotype
Summary: Microbial succession during postnatal gut development in mice is likely impacted by site of sampling, time, intestinal injury, and host genetics. We investigated this in wild-type and Sigirr transgenic mice that encode the p.Y168X mutation identified in a neonate with necrotizing enterocoli...
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| Language: | English |
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Elsevier
2025-04-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225005048 |
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| author | Shahid Umar Wei Yu Hao Xuan Ishfaq Ahmed Cuncong Zhong Michael Morowitz Mathew Brian Rogers Mark Ivan Attard Venkatesh Sampath |
| author_facet | Shahid Umar Wei Yu Hao Xuan Ishfaq Ahmed Cuncong Zhong Michael Morowitz Mathew Brian Rogers Mark Ivan Attard Venkatesh Sampath |
| author_sort | Shahid Umar |
| collection | DOAJ |
| description | Summary: Microbial succession during postnatal gut development in mice is likely impacted by site of sampling, time, intestinal injury, and host genetics. We investigated this in wild-type and Sigirr transgenic mice that encode the p.Y168X mutation identified in a neonate with necrotizing enterocolitis (NEC). Temporal profiling of the ileal and colonic microbiome after birth to weaning revealed a clear pattern of progression from a less diverse, Proteobacteria/Escherichia_Shigella dominant community to a more diverse, Firmicutes/Bacteroidetes dominant community. Formula milk feeding, a risk factor for necrotizing enterocolitis, decreased Firmicutes and increased Proteobacteria leading to enrichment of bacterial genes denoting exaggerated glycolysis and increased production of acetate and lactate. Sigirr transgenic mice exhibited modest baseline differences in microbiota composition but exaggerated formula feeding-induced dysbiosis, mucosal inflammation, and villus injury. Postnatal intestinal microbiota succession in mice resembles human neonates and is shaped by developmental maturity, ileal vs. colonic sampling, formula feeding, and Sigirr genotype. |
| format | Article |
| id | doaj-art-5f98b490f77f4ae69b04d74f01b51d24 |
| institution | DOAJ |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-5f98b490f77f4ae69b04d74f01b51d242025-08-20T03:08:50ZengElsevieriScience2589-00422025-04-0128411224310.1016/j.isci.2025.112243Neonatal gut microbiota succession in mice mapped over time, site, injury and single immunoglobulin interleukin-1 related receptor genotypeShahid Umar0Wei Yu1Hao Xuan2Ishfaq Ahmed3Cuncong Zhong4Michael Morowitz5Mathew Brian Rogers6Mark Ivan Attard7Venkatesh Sampath8Department of Surgery, University of Kansas Medical Center, USA; Corresponding authorDepartment of Pediatrics/Neonatology, Children’s Mercy Hospital, Kansas City, USADepartment of Electrical Engineering and Computer Science, University of Kansas, USADepartment of Math, Science and Computer Technology, Kansas City Community College, USADepartment of Electrical Engineering and Computer Science, University of Kansas, USADivision of Pediatric General and Thoracic Surgery, University of Pittsburgh Children’s Hospital, Pittsburgh, PA, USAVaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, CanadaNeonatal Unit, Aberdeen Maternity Hospital, Aberdeen AB25 2ZL, UKDepartment of Electrical Engineering and Computer Science, University of Kansas, USA; Corresponding authorSummary: Microbial succession during postnatal gut development in mice is likely impacted by site of sampling, time, intestinal injury, and host genetics. We investigated this in wild-type and Sigirr transgenic mice that encode the p.Y168X mutation identified in a neonate with necrotizing enterocolitis (NEC). Temporal profiling of the ileal and colonic microbiome after birth to weaning revealed a clear pattern of progression from a less diverse, Proteobacteria/Escherichia_Shigella dominant community to a more diverse, Firmicutes/Bacteroidetes dominant community. Formula milk feeding, a risk factor for necrotizing enterocolitis, decreased Firmicutes and increased Proteobacteria leading to enrichment of bacterial genes denoting exaggerated glycolysis and increased production of acetate and lactate. Sigirr transgenic mice exhibited modest baseline differences in microbiota composition but exaggerated formula feeding-induced dysbiosis, mucosal inflammation, and villus injury. Postnatal intestinal microbiota succession in mice resembles human neonates and is shaped by developmental maturity, ileal vs. colonic sampling, formula feeding, and Sigirr genotype.http://www.sciencedirect.com/science/article/pii/S2589004225005048ImmunologyMicrobiologyCell biology |
| spellingShingle | Shahid Umar Wei Yu Hao Xuan Ishfaq Ahmed Cuncong Zhong Michael Morowitz Mathew Brian Rogers Mark Ivan Attard Venkatesh Sampath Neonatal gut microbiota succession in mice mapped over time, site, injury and single immunoglobulin interleukin-1 related receptor genotype iScience Immunology Microbiology Cell biology |
| title | Neonatal gut microbiota succession in mice mapped over time, site, injury and single immunoglobulin interleukin-1 related receptor genotype |
| title_full | Neonatal gut microbiota succession in mice mapped over time, site, injury and single immunoglobulin interleukin-1 related receptor genotype |
| title_fullStr | Neonatal gut microbiota succession in mice mapped over time, site, injury and single immunoglobulin interleukin-1 related receptor genotype |
| title_full_unstemmed | Neonatal gut microbiota succession in mice mapped over time, site, injury and single immunoglobulin interleukin-1 related receptor genotype |
| title_short | Neonatal gut microbiota succession in mice mapped over time, site, injury and single immunoglobulin interleukin-1 related receptor genotype |
| title_sort | neonatal gut microbiota succession in mice mapped over time site injury and single immunoglobulin interleukin 1 related receptor genotype |
| topic | Immunology Microbiology Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225005048 |
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