Herpes simplex virus type-1 infection and spread in a novel porcine corneal explant model is restricted to the epithelium.
Herpes Keratitis (HK) is a debilitating infection of the cornea that remains the leading cause of infectious blindness in developed countries. Caused primarily by herpes simplex virus type 1 (HSV-1), it is associated with recurrent inflammation, leading to corneal scarring. This study investigated t...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-05-01
|
| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1013162 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849714645628092416 |
|---|---|
| author | Sana Arshad Hafsa Rana Naomi R Truong Ushasree Pattamatta Kirstie M Bertram Andrew White Holly R Chinnery Nicole A Carnt Anthony L Cunningham |
| author_facet | Sana Arshad Hafsa Rana Naomi R Truong Ushasree Pattamatta Kirstie M Bertram Andrew White Holly R Chinnery Nicole A Carnt Anthony L Cunningham |
| author_sort | Sana Arshad |
| collection | DOAJ |
| description | Herpes Keratitis (HK) is a debilitating infection of the cornea that remains the leading cause of infectious blindness in developed countries. Caused primarily by herpes simplex virus type 1 (HSV-1), it is associated with recurrent inflammation, leading to corneal scarring. This study investigated the initial events during acute HSV-1 infection in the cornea by adapting our human anogenital mucosal explant model to a HSV-1 infected porcine corneal explant model. We infected these corneas topically via high-density microarray patches (HD-MAPs) dipped in GFP-labelled HSV-1. Virus infection and spread was detected by both GFP protein and RNAscope, adapted for HSV-1 DNA. The punctures were consistent, usually in the epithelium but some extended into the underlying stroma. However, HSV-1 was restricted to the corneal epithelium, without spread through the anterior limiting membrane (ALM) or Bowman's layer into the stroma nor to the uppermost epithelial layer. This layer expressed SPRR1A similarly to the stratum granulosum of skin which is refractory to HSV-1 infection. In corneas where infected epithelial cells extended to the ALM, SPRR1A was also observed in this layer, suggesting it may contribute to its barrier function. Such studies of HSV-1 infection and spread will help improve therapy for HK and vaccine design to prevent it. |
| format | Article |
| id | doaj-art-5f87d4e5a20e464abbf7a6cf53025d3c |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-5f87d4e5a20e464abbf7a6cf53025d3c2025-08-20T03:13:39ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742025-05-01215e101316210.1371/journal.ppat.1013162Herpes simplex virus type-1 infection and spread in a novel porcine corneal explant model is restricted to the epithelium.Sana ArshadHafsa RanaNaomi R TruongUshasree PattamattaKirstie M BertramAndrew WhiteHolly R ChinneryNicole A CarntAnthony L CunninghamHerpes Keratitis (HK) is a debilitating infection of the cornea that remains the leading cause of infectious blindness in developed countries. Caused primarily by herpes simplex virus type 1 (HSV-1), it is associated with recurrent inflammation, leading to corneal scarring. This study investigated the initial events during acute HSV-1 infection in the cornea by adapting our human anogenital mucosal explant model to a HSV-1 infected porcine corneal explant model. We infected these corneas topically via high-density microarray patches (HD-MAPs) dipped in GFP-labelled HSV-1. Virus infection and spread was detected by both GFP protein and RNAscope, adapted for HSV-1 DNA. The punctures were consistent, usually in the epithelium but some extended into the underlying stroma. However, HSV-1 was restricted to the corneal epithelium, without spread through the anterior limiting membrane (ALM) or Bowman's layer into the stroma nor to the uppermost epithelial layer. This layer expressed SPRR1A similarly to the stratum granulosum of skin which is refractory to HSV-1 infection. In corneas where infected epithelial cells extended to the ALM, SPRR1A was also observed in this layer, suggesting it may contribute to its barrier function. Such studies of HSV-1 infection and spread will help improve therapy for HK and vaccine design to prevent it.https://doi.org/10.1371/journal.ppat.1013162 |
| spellingShingle | Sana Arshad Hafsa Rana Naomi R Truong Ushasree Pattamatta Kirstie M Bertram Andrew White Holly R Chinnery Nicole A Carnt Anthony L Cunningham Herpes simplex virus type-1 infection and spread in a novel porcine corneal explant model is restricted to the epithelium. PLoS Pathogens |
| title | Herpes simplex virus type-1 infection and spread in a novel porcine corneal explant model is restricted to the epithelium. |
| title_full | Herpes simplex virus type-1 infection and spread in a novel porcine corneal explant model is restricted to the epithelium. |
| title_fullStr | Herpes simplex virus type-1 infection and spread in a novel porcine corneal explant model is restricted to the epithelium. |
| title_full_unstemmed | Herpes simplex virus type-1 infection and spread in a novel porcine corneal explant model is restricted to the epithelium. |
| title_short | Herpes simplex virus type-1 infection and spread in a novel porcine corneal explant model is restricted to the epithelium. |
| title_sort | herpes simplex virus type 1 infection and spread in a novel porcine corneal explant model is restricted to the epithelium |
| url | https://doi.org/10.1371/journal.ppat.1013162 |
| work_keys_str_mv | AT sanaarshad herpessimplexvirustype1infectionandspreadinanovelporcinecornealexplantmodelisrestrictedtotheepithelium AT hafsarana herpessimplexvirustype1infectionandspreadinanovelporcinecornealexplantmodelisrestrictedtotheepithelium AT naomirtruong herpessimplexvirustype1infectionandspreadinanovelporcinecornealexplantmodelisrestrictedtotheepithelium AT ushasreepattamatta herpessimplexvirustype1infectionandspreadinanovelporcinecornealexplantmodelisrestrictedtotheepithelium AT kirstiembertram herpessimplexvirustype1infectionandspreadinanovelporcinecornealexplantmodelisrestrictedtotheepithelium AT andrewwhite herpessimplexvirustype1infectionandspreadinanovelporcinecornealexplantmodelisrestrictedtotheepithelium AT hollyrchinnery herpessimplexvirustype1infectionandspreadinanovelporcinecornealexplantmodelisrestrictedtotheepithelium AT nicoleacarnt herpessimplexvirustype1infectionandspreadinanovelporcinecornealexplantmodelisrestrictedtotheepithelium AT anthonylcunningham herpessimplexvirustype1infectionandspreadinanovelporcinecornealexplantmodelisrestrictedtotheepithelium |