Exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo.
Exercise has beneficial effects on metabolism and health. Although the skeletal muscle has been a primary focus, exercise also mediates robust adaptations in white adipose tissue. To determine if exercise affects in vivo adipocyte formation, fifty-two, sixteen-week-old C57BL/6J mice were allowed acc...
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0244804&type=printable |
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| author | Timothy D Allerton Jonathan J Savoie Mark D Fitch Marc K Hellerstein Jacqueline M Stephens Ursula White |
| author_facet | Timothy D Allerton Jonathan J Savoie Mark D Fitch Marc K Hellerstein Jacqueline M Stephens Ursula White |
| author_sort | Timothy D Allerton |
| collection | DOAJ |
| description | Exercise has beneficial effects on metabolism and health. Although the skeletal muscle has been a primary focus, exercise also mediates robust adaptations in white adipose tissue. To determine if exercise affects in vivo adipocyte formation, fifty-two, sixteen-week-old C57BL/6J mice were allowed access to unlocked running wheels [Exercise (EX) group; n = 13 males, n = 13 females] or to locked wheels [Sedentary (SED) group; n = 13 males, n = 13 females] for 4-weeks. In vivo adipocyte formation was assessed by the incorporation of deuterium (2H) into the DNA of newly formed adipocytes in the inguinal and gonadal adipose depots. A two-way ANOVA revealed that exercise significantly decreased new adipocyte formation in the adipose tissue of mice in the EX group relative to the SED group (activity effect; P = 0.02). This reduction was observed in male and female mice (activity effect; P = 0.03). Independent analysis of the depots showed a significant reduction in adipocyte formation in the inguinal (P = 0.05) but not in the gonadal (P = 0.18) of the EX group. We report for the first time that exercise significantly reduced in vivo adipocyte formation in the adipose tissue of EX mice using a physiologic metabolic 2H2O-labeling protocol. |
| format | Article |
| id | doaj-art-5f86e9f0877c404ebcebfb02c58d442e |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-5f86e9f0877c404ebcebfb02c58d442e2025-01-24T05:31:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01161e024480410.1371/journal.pone.0244804Exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo.Timothy D AllertonJonathan J SavoieMark D FitchMarc K HellersteinJacqueline M StephensUrsula WhiteExercise has beneficial effects on metabolism and health. Although the skeletal muscle has been a primary focus, exercise also mediates robust adaptations in white adipose tissue. To determine if exercise affects in vivo adipocyte formation, fifty-two, sixteen-week-old C57BL/6J mice were allowed access to unlocked running wheels [Exercise (EX) group; n = 13 males, n = 13 females] or to locked wheels [Sedentary (SED) group; n = 13 males, n = 13 females] for 4-weeks. In vivo adipocyte formation was assessed by the incorporation of deuterium (2H) into the DNA of newly formed adipocytes in the inguinal and gonadal adipose depots. A two-way ANOVA revealed that exercise significantly decreased new adipocyte formation in the adipose tissue of mice in the EX group relative to the SED group (activity effect; P = 0.02). This reduction was observed in male and female mice (activity effect; P = 0.03). Independent analysis of the depots showed a significant reduction in adipocyte formation in the inguinal (P = 0.05) but not in the gonadal (P = 0.18) of the EX group. We report for the first time that exercise significantly reduced in vivo adipocyte formation in the adipose tissue of EX mice using a physiologic metabolic 2H2O-labeling protocol.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0244804&type=printable |
| spellingShingle | Timothy D Allerton Jonathan J Savoie Mark D Fitch Marc K Hellerstein Jacqueline M Stephens Ursula White Exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo. PLoS ONE |
| title | Exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo. |
| title_full | Exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo. |
| title_fullStr | Exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo. |
| title_full_unstemmed | Exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo. |
| title_short | Exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo. |
| title_sort | exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0244804&type=printable |
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