Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease Patients
The microbiota of the gastrointestinal tract is frequently mentioned as one of the key players in the etiopathogenesis of Crohn's disease (CD). Four hypotheses have been suggested: the single, still unknown bacterial pathogen, an abnormal overall composition of the bowel microbiota (“dysbiosis...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/505674 |
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| _version_ | 1850159115939086336 |
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| author | Gert De Hertogh Bart Lemmens Peter Verhasselt Ronald de Hoogt Xavier Sagaert Marie Joossens Gert Van Assche Paul Rutgeerts Severine Vermeire Jeroen Aerssens |
| author_facet | Gert De Hertogh Bart Lemmens Peter Verhasselt Ronald de Hoogt Xavier Sagaert Marie Joossens Gert Van Assche Paul Rutgeerts Severine Vermeire Jeroen Aerssens |
| author_sort | Gert De Hertogh |
| collection | DOAJ |
| description | The microbiota of the gastrointestinal tract is frequently mentioned as one of the key players in the etiopathogenesis of Crohn's disease (CD). Four hypotheses have been suggested: the single, still unknown bacterial pathogen, an abnormal overall composition of the bowel microbiota (“dysbiosis”), an abnormal immunological reaction to an essentially normally composed microbiota, and increased bacterial translocation. We propose that laser capture microdissection of selected microscopic structures, followed by broad-range 16S rRNA gene sequencing, is an excellent method to assess spatiotemporal alterations in the composition of the bowel microbiota in CD. Using this approach, we demonstrated significant changes of the composition, abundance, and location of the gut microbiome in this disease. Some of these abnormal findings persisted even after macroscopic mucosal healing. Further investigations along these lines may lead to a better understanding of the possible involvement of the bowel bacteria in the development of clinical Crohn's disease. |
| format | Article |
| id | doaj-art-5f835aebd7d041de993f3ccae034e4ea |
| institution | OA Journals |
| issn | 2090-8040 2042-0099 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-5f835aebd7d041de993f3ccae034e4ea2025-08-20T02:23:40ZengWileyInternational Journal of Inflammation2090-80402042-00992012-01-01201210.1155/2012/505674505674Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease PatientsGert De Hertogh0Bart Lemmens1Peter Verhasselt2Ronald de Hoogt3Xavier Sagaert4Marie Joossens5Gert Van Assche6Paul Rutgeerts7Severine Vermeire8Jeroen Aerssens9Department of Morphology and Molecular Pathology, University Hospitals KULeuven, Minderbroedersstraat 12, 3000 Leuven, BelgiumDepartment of Morphology and Molecular Pathology, University Hospitals KULeuven, Minderbroedersstraat 12, 3000 Leuven, BelgiumDepartment Research Informatics & Integrated Genomics, Janssen Pharmaceutical Companies of Johnson & Johnson, Turnhoutseweg 30, 2340 Beerse, BelgiumDepartment Research Informatics & Integrated Genomics, Janssen Pharmaceutical Companies of Johnson & Johnson, Turnhoutseweg 30, 2340 Beerse, BelgiumDepartment of Morphology and Molecular Pathology, University Hospitals KULeuven, Minderbroedersstraat 12, 3000 Leuven, BelgiumDepartment of Gastroenterology, University Hospitals KULeuven, Herestraat 49, 3000 Leuven, BelgiumDepartment of Gastroenterology, University Hospitals KULeuven, Herestraat 49, 3000 Leuven, BelgiumDepartment of Gastroenterology, University Hospitals KULeuven, Herestraat 49, 3000 Leuven, BelgiumDepartment of Gastroenterology, University Hospitals KULeuven, Herestraat 49, 3000 Leuven, BelgiumDepartment Research Informatics & Integrated Genomics, Janssen Pharmaceutical Companies of Johnson & Johnson, Turnhoutseweg 30, 2340 Beerse, BelgiumThe microbiota of the gastrointestinal tract is frequently mentioned as one of the key players in the etiopathogenesis of Crohn's disease (CD). Four hypotheses have been suggested: the single, still unknown bacterial pathogen, an abnormal overall composition of the bowel microbiota (“dysbiosis”), an abnormal immunological reaction to an essentially normally composed microbiota, and increased bacterial translocation. We propose that laser capture microdissection of selected microscopic structures, followed by broad-range 16S rRNA gene sequencing, is an excellent method to assess spatiotemporal alterations in the composition of the bowel microbiota in CD. Using this approach, we demonstrated significant changes of the composition, abundance, and location of the gut microbiome in this disease. Some of these abnormal findings persisted even after macroscopic mucosal healing. Further investigations along these lines may lead to a better understanding of the possible involvement of the bowel bacteria in the development of clinical Crohn's disease.http://dx.doi.org/10.1155/2012/505674 |
| spellingShingle | Gert De Hertogh Bart Lemmens Peter Verhasselt Ronald de Hoogt Xavier Sagaert Marie Joossens Gert Van Assche Paul Rutgeerts Severine Vermeire Jeroen Aerssens Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease Patients International Journal of Inflammation |
| title | Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease Patients |
| title_full | Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease Patients |
| title_fullStr | Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease Patients |
| title_full_unstemmed | Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease Patients |
| title_short | Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease Patients |
| title_sort | assessment of the microbiota in microdissected tissues of crohn s disease patients |
| url | http://dx.doi.org/10.1155/2012/505674 |
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