A whole genome analysis of two coxsackievirus A2 strains isolated from patients with herpetic angina in Shanghai
ObjectiveTo understand the whole genome characteristics and the information for genetic evolution in the two coxsackievirus A2 (CVA2) strains isolated from patients with herpangina in Shanghai, and to provide a scientific basis for the prevention and treatment of herpetic angina.MethodsTwo CAV2 stra...
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Shanghai Preventive Medicine Association
2025-03-01
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| Series: | Shanghai yufang yixue |
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| Online Access: | http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2025.24306 |
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| author | ZHANG Jingyi WANG Jiayu LI Run FANG Fanghao WU Wencheng ZHANG Wanju CHEN Min CHEN Xin TENG Zheng |
| author_facet | ZHANG Jingyi WANG Jiayu LI Run FANG Fanghao WU Wencheng ZHANG Wanju CHEN Min CHEN Xin TENG Zheng |
| author_sort | ZHANG Jingyi |
| collection | DOAJ |
| description | ObjectiveTo understand the whole genome characteristics and the information for genetic evolution in the two coxsackievirus A2 (CVA2) strains isolated from patients with herpangina in Shanghai, and to provide a scientific basis for the prevention and treatment of herpetic angina.MethodsTwo CAV2 strains isolated from patients with herpetic angina in Shanghai were performed whole genome sequencing and analysis for phylogenetics, nucleotide homology, and evolution.ResultsA phylogenetic analysis of the VP1 region revealed that the two Shanghai strains both belonged to CVA2 genotype D, with the highest homology to OL357660, a strain from Yunnan. The average nucleotide identity (ANI) of the whole genome between the two Shanghai strains was 98.88%, and the ANI of the whole genome comparisons to other CVA2 genotype D strains and CVA2 genotypes A-C strains ranged from 84.64% to 97.42% and from 79.21% to 84.20%, respectively. The two Shanghai strains had low homology in the 3D region compared to the existing CVA2 strains. The phylogenetic analysis and sliding window nucleotide similarity analysis indicated that the two Shanghai strains and the Yunnan OL357660 strain might constitute a new genetic lineage.ConclusionThe two CVA2 strains isolated for the first time in Shanghai are assigned to genotype D (GenBank: PQ130039 and PQ130040), which is identical to the existing subtype prevalent in China. As represented by the Shanghai strains, a new CVA2 genetic lineage is been identified. This study has enriched the data on genetic evolution and genetic variation of CVA2 in Shanghai, indicating the requirement to strengthen surveillance for the epidemiological pattern of CVA2. |
| format | Article |
| id | doaj-art-5f7b54287c5d4b649c3790d3072e04fb |
| institution | Kabale University |
| issn | 1004-9231 |
| language | zho |
| publishDate | 2025-03-01 |
| publisher | Shanghai Preventive Medicine Association |
| record_format | Article |
| series | Shanghai yufang yixue |
| spelling | doaj-art-5f7b54287c5d4b649c3790d3072e04fb2025-08-20T03:31:21ZzhoShanghai Preventive Medicine AssociationShanghai yufang yixue1004-92312025-03-0137321522110.19428/j.cnki.sjpm.2025.243061004-9231（2025）03-0215-07A whole genome analysis of two coxsackievirus A2 strains isolated from patients with herpetic angina in ShanghaiZHANG Jingyi0WANG Jiayu1LI Run2FANG Fanghao3WU Wencheng4ZHANG Wanju5CHEN Min6CHEN Xin7TENG Zheng8Institute of Microbiology Laboratory, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaInstitute of Microbiology Laboratory, Shanghai Institute of Preventive Medicine, Shanghai 200336, ChinaInstitute of Microbiology Laboratory, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaInstitute of Microbiology Laboratory, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaInstitute of Microbiology Laboratory, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaInstitute of Microbiology Laboratory, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaInstitute of Microbiology Laboratory, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaInstitute of Microbiology Laboratory, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaInstitute of Microbiology Laboratory, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaObjectiveTo understand the whole genome characteristics and the information for genetic evolution in the two coxsackievirus A2 (CVA2) strains isolated from patients with herpangina in Shanghai, and to provide a scientific basis for the prevention and treatment of herpetic angina.MethodsTwo CAV2 strains isolated from patients with herpetic angina in Shanghai were performed whole genome sequencing and analysis for phylogenetics, nucleotide homology, and evolution.ResultsA phylogenetic analysis of the VP1 region revealed that the two Shanghai strains both belonged to CVA2 genotype D, with the highest homology to OL357660, a strain from Yunnan. The average nucleotide identity (ANI) of the whole genome between the two Shanghai strains was 98.88%, and the ANI of the whole genome comparisons to other CVA2 genotype D strains and CVA2 genotypes A-C strains ranged from 84.64% to 97.42% and from 79.21% to 84.20%, respectively. The two Shanghai strains had low homology in the 3D region compared to the existing CVA2 strains. The phylogenetic analysis and sliding window nucleotide similarity analysis indicated that the two Shanghai strains and the Yunnan OL357660 strain might constitute a new genetic lineage.ConclusionThe two CVA2 strains isolated for the first time in Shanghai are assigned to genotype D (GenBank: PQ130039 and PQ130040), which is identical to the existing subtype prevalent in China. As represented by the Shanghai strains, a new CVA2 genetic lineage is been identified. This study has enriched the data on genetic evolution and genetic variation of CVA2 in Shanghai, indicating the requirement to strengthen surveillance for the epidemiological pattern of CVA2.http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2025.24306coxsackievirus a2herpetic anginawhole genome analysisgenetic characteristic |
| spellingShingle | ZHANG Jingyi WANG Jiayu LI Run FANG Fanghao WU Wencheng ZHANG Wanju CHEN Min CHEN Xin TENG Zheng A whole genome analysis of two coxsackievirus A2 strains isolated from patients with herpetic angina in Shanghai Shanghai yufang yixue coxsackievirus a2 herpetic angina whole genome analysis genetic characteristic |
| title | A whole genome analysis of two coxsackievirus A2 strains isolated from patients with herpetic angina in Shanghai |
| title_full | A whole genome analysis of two coxsackievirus A2 strains isolated from patients with herpetic angina in Shanghai |
| title_fullStr | A whole genome analysis of two coxsackievirus A2 strains isolated from patients with herpetic angina in Shanghai |
| title_full_unstemmed | A whole genome analysis of two coxsackievirus A2 strains isolated from patients with herpetic angina in Shanghai |
| title_short | A whole genome analysis of two coxsackievirus A2 strains isolated from patients with herpetic angina in Shanghai |
| title_sort | whole genome analysis of two coxsackievirus a2 strains isolated from patients with herpetic angina in shanghai |
| topic | coxsackievirus a2 herpetic angina whole genome analysis genetic characteristic |
| url | http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2025.24306 |
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