Cellular viability in an in vitro model of human ventricular cardiomyocytes (RL-14) exposed to gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste
In nanotechnology, tissue engineering proposes obtaining nanomaterials of natural or synthetic origin, looking to incorporate components that exhibit a defined shape, diameter, colloidal stability, and biological identity to promote and regulate the events that occur in a cardiac cell microenvironme...
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Elsevier
2024-11-01
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| Series: | OpenNano |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352952024000197 |
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| author | Yuliet Montoya Wilson Agudelo Alejandra Garcia-Garcia John Bustamante |
| author_facet | Yuliet Montoya Wilson Agudelo Alejandra Garcia-Garcia John Bustamante |
| author_sort | Yuliet Montoya |
| collection | DOAJ |
| description | In nanotechnology, tissue engineering proposes obtaining nanomaterials of natural or synthetic origin, looking to incorporate components that exhibit a defined shape, diameter, colloidal stability, and biological identity to promote and regulate the events that occur in a cardiac cell microenvironment. This research aimed to evaluate cellular viability in an in vitro model of human fetal ventricular cardiomyocytes on interaction with gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste. The Physicochemical properties were characterized by UV–visible spectroscopy, Fourier-transform infrared spectroscopy, electrokinetic potential, and scanning transmission electron microscopy. Moreover, the MTT assay was used to determine the cell viability of cardiomyocytes exposed to gold nanoparticles. The results showed that the variation of the pH of the reaction allows the synthesis of different geometries of nanoparticles with diameters between 6 and 334 nm. Furthermore, it was found that the nanoparticles with a tendency to sphericity favor the cell viability of cardiomyocytes. |
| format | Article |
| id | doaj-art-5f7557442f93409da50d998eb4a68415 |
| institution | OA Journals |
| issn | 2352-9520 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | OpenNano |
| spelling | doaj-art-5f7557442f93409da50d998eb4a684152025-08-20T02:05:31ZengElsevierOpenNano2352-95202024-11-012010021810.1016/j.onano.2024.100218Cellular viability in an in vitro model of human ventricular cardiomyocytes (RL-14) exposed to gold nanoparticles biosynthesized using silk fibroin from silk fibrous wasteYuliet Montoya0Wilson Agudelo1Alejandra Garcia-Garcia2John Bustamante3Grupo de Dinámica Cardiovascular, Centro de Bioingeniería, Universidad Pontificia Bolivariana, Medellín, Colombia; Comité de Trabajo de Bioingeniería Cardiovascular, Sociedad Colombiana de Cardiología y Cirugía Cardiovascular, Bogotá, Colombia; Corresponding author.Grupo de Dinámica Cardiovascular, Centro de Bioingeniería, Universidad Pontificia Bolivariana, Medellín, ColombiaCentro de Investigación en Materiales Avanzados S.C, Apodaca N. L, Parque PIIT Alianza Norte 202, Apodaca N. L., MéxicoGrupo de Dinámica Cardiovascular, Centro de Bioingeniería, Universidad Pontificia Bolivariana, Medellín, Colombia; Comité de Trabajo de Bioingeniería Cardiovascular, Sociedad Colombiana de Cardiología y Cirugía Cardiovascular, Bogotá, ColombiaIn nanotechnology, tissue engineering proposes obtaining nanomaterials of natural or synthetic origin, looking to incorporate components that exhibit a defined shape, diameter, colloidal stability, and biological identity to promote and regulate the events that occur in a cardiac cell microenvironment. This research aimed to evaluate cellular viability in an in vitro model of human fetal ventricular cardiomyocytes on interaction with gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste. The Physicochemical properties were characterized by UV–visible spectroscopy, Fourier-transform infrared spectroscopy, electrokinetic potential, and scanning transmission electron microscopy. Moreover, the MTT assay was used to determine the cell viability of cardiomyocytes exposed to gold nanoparticles. The results showed that the variation of the pH of the reaction allows the synthesis of different geometries of nanoparticles with diameters between 6 and 334 nm. Furthermore, it was found that the nanoparticles with a tendency to sphericity favor the cell viability of cardiomyocytes.http://www.sciencedirect.com/science/article/pii/S2352952024000197Cellular viabilityRL14 human cardiomyocytesGold nanoparticlesSilk fibroin from silk fibrous wasteBiosynthesis |
| spellingShingle | Yuliet Montoya Wilson Agudelo Alejandra Garcia-Garcia John Bustamante Cellular viability in an in vitro model of human ventricular cardiomyocytes (RL-14) exposed to gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste OpenNano Cellular viability RL14 human cardiomyocytes Gold nanoparticles Silk fibroin from silk fibrous waste Biosynthesis |
| title | Cellular viability in an in vitro model of human ventricular cardiomyocytes (RL-14) exposed to gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste |
| title_full | Cellular viability in an in vitro model of human ventricular cardiomyocytes (RL-14) exposed to gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste |
| title_fullStr | Cellular viability in an in vitro model of human ventricular cardiomyocytes (RL-14) exposed to gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste |
| title_full_unstemmed | Cellular viability in an in vitro model of human ventricular cardiomyocytes (RL-14) exposed to gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste |
| title_short | Cellular viability in an in vitro model of human ventricular cardiomyocytes (RL-14) exposed to gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste |
| title_sort | cellular viability in an in vitro model of human ventricular cardiomyocytes rl 14 exposed to gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste |
| topic | Cellular viability RL14 human cardiomyocytes Gold nanoparticles Silk fibroin from silk fibrous waste Biosynthesis |
| url | http://www.sciencedirect.com/science/article/pii/S2352952024000197 |
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