Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRB
Aim. The study was to investigate the metabolic profile of urine metabolites and to elucidate their clinical significance in patients with colorectal cancer. Methods. Colorectal cancers from early stage and advanced stage were used in this study. Urine samples of colorectal cancer patients and heal...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2012/125890 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548058151256064 |
|---|---|
| author | Jin-Lian Chen Jing Fan Liu-Shui Yan Hui-Qin Guo Jing-Jing Xiong Yan Ren Jun-Duo Hu |
| author_facet | Jin-Lian Chen Jing Fan Liu-Shui Yan Hui-Qin Guo Jing-Jing Xiong Yan Ren Jun-Duo Hu |
| author_sort | Jin-Lian Chen |
| collection | DOAJ |
| description | Aim. The study was to investigate the metabolic profile of urine metabolites and to elucidate their clinical significance in patients with colorectal cancer.
Methods. Colorectal cancers from early stage and advanced stage were used in this study. Urine samples of colorectal cancer patients and healthy adults were collected and subjected to capillary
electrophoresis mass spectrometry based on moving reaction boundary analysis. The metabolic data were analyzed by SPSS 17.0 to find urinary biomarkers for colorectal cancer.
Results. The results indicated that the urine metabolic profiling of colorectal cancer patients had significant changes compared with the normal controls, and there were also differences between early stage and advanced colorectal cancer patients. Compared with the control group, the levels of isoleucine, valine, arginine, lactate acid and leucine increased (P<0.05), but those of histidine, methionine, serine, aspartic acid, citric acid, succinate, and malic acid decreased in urine samples from colorectal cancer (P<0.05). Furthermore, the levels of isoleucine and valine were lower in urine of patients with advanced colorectal cancer than those in early stage colorectal cancer
(P<0.05). Conclusion. The technique of capillary electrophoresis mass spectrometry based on MRB could reveal the significant metabolic alterations during progression of colorectal cancer, and the method is feasible and may be useful for the early diagnosis of colorectal cancer. |
| format | Article |
| id | doaj-art-5f6efcc1bfcc42deb8e0455d489d2478 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-5f6efcc1bfcc42deb8e0455d489d24782025-02-03T06:42:18ZengWileyGastroenterology Research and Practice1687-61211687-630X2012-01-01201210.1155/2012/125890125890Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRBJin-Lian Chen0Jing Fan1Liu-Shui Yan2Hui-Qin Guo3Jing-Jing Xiong4Yan Ren5Jun-Duo Hu6Department of Gastroenterology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, ChinaDepartment of Gastroenterology, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, ChinaCyclization College, Nanchang Aeronautical University, Jiangxi, Nanchang 330063, ChinaCyclization College, Nanchang Aeronautical University, Jiangxi, Nanchang 330063, ChinaCyclization College, Nanchang Aeronautical University, Jiangxi, Nanchang 330063, ChinaCyclization College, Nanchang Aeronautical University, Jiangxi, Nanchang 330063, ChinaDepartment of Gastroenterology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, ChinaAim. The study was to investigate the metabolic profile of urine metabolites and to elucidate their clinical significance in patients with colorectal cancer. Methods. Colorectal cancers from early stage and advanced stage were used in this study. Urine samples of colorectal cancer patients and healthy adults were collected and subjected to capillary electrophoresis mass spectrometry based on moving reaction boundary analysis. The metabolic data were analyzed by SPSS 17.0 to find urinary biomarkers for colorectal cancer. Results. The results indicated that the urine metabolic profiling of colorectal cancer patients had significant changes compared with the normal controls, and there were also differences between early stage and advanced colorectal cancer patients. Compared with the control group, the levels of isoleucine, valine, arginine, lactate acid and leucine increased (P<0.05), but those of histidine, methionine, serine, aspartic acid, citric acid, succinate, and malic acid decreased in urine samples from colorectal cancer (P<0.05). Furthermore, the levels of isoleucine and valine were lower in urine of patients with advanced colorectal cancer than those in early stage colorectal cancer (P<0.05). Conclusion. The technique of capillary electrophoresis mass spectrometry based on MRB could reveal the significant metabolic alterations during progression of colorectal cancer, and the method is feasible and may be useful for the early diagnosis of colorectal cancer.http://dx.doi.org/10.1155/2012/125890 |
| spellingShingle | Jin-Lian Chen Jing Fan Liu-Shui Yan Hui-Qin Guo Jing-Jing Xiong Yan Ren Jun-Duo Hu Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRB Gastroenterology Research and Practice |
| title | Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRB |
| title_full | Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRB |
| title_fullStr | Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRB |
| title_full_unstemmed | Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRB |
| title_short | Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRB |
| title_sort | urine metabolite profiling of human colorectal cancer by capillary electrophoresis mass spectrometry based on mrb |
| url | http://dx.doi.org/10.1155/2012/125890 |
| work_keys_str_mv | AT jinlianchen urinemetaboliteprofilingofhumancolorectalcancerbycapillaryelectrophoresismassspectrometrybasedonmrb AT jingfan urinemetaboliteprofilingofhumancolorectalcancerbycapillaryelectrophoresismassspectrometrybasedonmrb AT liushuiyan urinemetaboliteprofilingofhumancolorectalcancerbycapillaryelectrophoresismassspectrometrybasedonmrb AT huiqinguo urinemetaboliteprofilingofhumancolorectalcancerbycapillaryelectrophoresismassspectrometrybasedonmrb AT jingjingxiong urinemetaboliteprofilingofhumancolorectalcancerbycapillaryelectrophoresismassspectrometrybasedonmrb AT yanren urinemetaboliteprofilingofhumancolorectalcancerbycapillaryelectrophoresismassspectrometrybasedonmrb AT junduohu urinemetaboliteprofilingofhumancolorectalcancerbycapillaryelectrophoresismassspectrometrybasedonmrb |