Glutathione triggers leaf-to-leaf, calcium-based plant defense signaling

Abstract Animals rely on nervous systems to cope with environmental variability, whereas plants are characterized by lack of nervous system but still have evolved systemic communication systems through signaling molecules that trigger long-distance defense signaling events when encountered with envi...

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Main Authors: Rui Li, Yongfang Yang, Hao Lou, Weicheng Wang, Ran Du, Haidong Chen, Xiaoxi Du, Shuai Hu, Guo-Liang Wang, Jianbin Yan, Xiaoyi Shan, Daoxin Xie
Format: Article
Language:English
Published: Nature Portfolio 2025-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-57239-1
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Summary:Abstract Animals rely on nervous systems to cope with environmental variability, whereas plants are characterized by lack of nervous system but still have evolved systemic communication systems through signaling molecules that trigger long-distance defense signaling events when encountered with environmental challenges. Here, our genetic screening of the previously constructed hairpin RNA-based Arabidopsis library identifies a glutathione (GSH)-deficient mutant that has high accumulation of glutamate (Glu), a previously defined wound signal essential for activating plant defense, but disharmoniously exhibits attenuation of defense signaling events. We further uncover GSH as a critical signaling molecule that relies on GLUTAMATE RECEPTOR-LIKE 3.3 (GLR3.3) to trigger long-distance calcium-based defense signaling events in plants. Our findings offer new insights into highly sophisticated systemic defense systems evolved by plants to defend against herbivory and pathogen invasion.
ISSN:2041-1723