Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome
In Saccharomyces cerevisiae, polyadenylated forms of mature (and not precur-sor) small non-coding RNAs (sncRNAs) those fail to undergo proper 3-end mat-uration are subject to an active degradation by Rrp6p and Rrp47p, which does not require the involvement of core exosome and TRAMP components. In a...
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2024-05-01
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| author | Anusha Chaudhuri Soumita Paul Mayukh Banerjea Biswadip Das |
| author_facet | Anusha Chaudhuri Soumita Paul Mayukh Banerjea Biswadip Das |
| author_sort | Anusha Chaudhuri |
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| description | In Saccharomyces cerevisiae, polyadenylated forms of mature (and not precur-sor) small non-coding RNAs (sncRNAs) those fail to undergo proper 3-end mat-uration are subject to an active degradation by Rrp6p and Rrp47p, which does not require the involvement of core exosome and TRAMP components. In agreement with this finding, Rrp6p/Rrp47p is demonstrated to exist as an exo-some-independent complex, which preferentially associates with mature poly-adenylated forms of these sncRNAs. Consistent with this observation, a C-terminally truncated version of Rrp6p (Rrp6p-ΔC2) lacking physical association with the core nuclear exosome supports their decay just like its full-length ver-sion. Polyadenylation is catalyzed by both the canonical and non-canonical poly(A) polymerases, Pap1p and Trf4p. Analysis of the polyadenylation profiles in WT and rrp6-Δ strains revealed that the majority of the polyadenylation sites correspond to either one to three nucleotides upstream or downstream of their mature ends and their poly(A) tails ranges from 10-15 adenylate residues. Most interestingly, the accumulated polyadenylated snRNAs are functional in the rrp6-Δ strain and are assembled into spliceosomes. Thus, Rrp6p-Rrp47p defines a core nuclear exosome-independent novel RNA turnover system in baker’s yeast targeting imperfectly processed polyadenylated sncRNAs that accumu-late in the absence of Rrp6p. |
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| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2024-05-01 |
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| series | Microbial Cell |
| spelling | doaj-art-5f5ae54a84e24023843797e2aeaca3b72025-08-20T02:52:37ZengShared Science Publishers OGMicrobial Cell2311-26382024-05-011115518610.15698/mic2024.05.823Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosomeAnusha Chaudhuri0Soumita Paul1Mayukh Banerjea2Biswadip Das3Present Position: Zentrum fǜr Molekulare, Medizin, Institut fǜr Kardiovaskuläre Regeneration, Haus 25B, Goethe-Universität, Theodor -Stern-Kai 7, Universitätsklinikum, 60590 Frankfurt am Main, Germany.Department of Life Science and Biotechnology, Jadavpur University, 188 Raja S.C. Mullick Road, Kolkata – 700 032, West Bengal, India.Department of Life Science and Biotechnology, Jadavpur University, 188 Raja S.C. Mullick Road, Kolkata – 700 032, West Bengal, India.Department of Life Science and Biotechnology, Jadavpur University, 188 Raja S.C. Mullick Road, Kolkata – 700 032, West Bengal, India.In Saccharomyces cerevisiae, polyadenylated forms of mature (and not precur-sor) small non-coding RNAs (sncRNAs) those fail to undergo proper 3-end mat-uration are subject to an active degradation by Rrp6p and Rrp47p, which does not require the involvement of core exosome and TRAMP components. In agreement with this finding, Rrp6p/Rrp47p is demonstrated to exist as an exo-some-independent complex, which preferentially associates with mature poly-adenylated forms of these sncRNAs. Consistent with this observation, a C-terminally truncated version of Rrp6p (Rrp6p-ΔC2) lacking physical association with the core nuclear exosome supports their decay just like its full-length ver-sion. Polyadenylation is catalyzed by both the canonical and non-canonical poly(A) polymerases, Pap1p and Trf4p. Analysis of the polyadenylation profiles in WT and rrp6-Δ strains revealed that the majority of the polyadenylation sites correspond to either one to three nucleotides upstream or downstream of their mature ends and their poly(A) tails ranges from 10-15 adenylate residues. Most interestingly, the accumulated polyadenylated snRNAs are functional in the rrp6-Δ strain and are assembled into spliceosomes. Thus, Rrp6p-Rrp47p defines a core nuclear exosome-independent novel RNA turnover system in baker’s yeast targeting imperfectly processed polyadenylated sncRNAs that accumu-late in the absence of Rrp6p.http://microbialcell.com/researcharticles/2024a-chaudhuri-microbial-cell/rrnasnornasnrnanuclear exosomerrp6prrp47pmpp6pnuclear rna turnover |
| spellingShingle | Anusha Chaudhuri Soumita Paul Mayukh Banerjea Biswadip Das Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome Microbial Cell rrna snorna snrna nuclear exosome rrp6p rrp47p mpp6p nuclear rna turnover |
| title | Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome |
| title_full | Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome |
| title_fullStr | Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome |
| title_full_unstemmed | Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome |
| title_short | Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome |
| title_sort | polyadenylated versions of small non coding rnas in saccharomyces cerevisiae are degraded by rrp6p rrp47p independent of the core nuclear exosome |
| topic | rrna snorna snrna nuclear exosome rrp6p rrp47p mpp6p nuclear rna turnover |
| url | http://microbialcell.com/researcharticles/2024a-chaudhuri-microbial-cell/ |
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