IMPACT OF TRANSCRIPTION FACTORS, VEGF AND PROTEASES ON KIDNEY CANCER PROGRESSION
Introduction. The efficacy of anticancer treatment depends on biological factors of tumor.The aim of the study was to determine the activity of proteasomes and calpains and to reveal their association with VEGF, HIF-1α and NF-κΒ expressions in normal, primary and metastatic renal cell carcinoma (RC...
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, Tomsk National Research Medical Center
2018-09-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/818 |
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| author | L. V. Spirina I. V. Kondakova E. A. Usynin E. M. Slonimskaya Z. A. Yurmazov |
| author_facet | L. V. Spirina I. V. Kondakova E. A. Usynin E. M. Slonimskaya Z. A. Yurmazov |
| author_sort | L. V. Spirina |
| collection | DOAJ |
| description | Introduction. The efficacy of anticancer treatment depends on biological factors of tumor.The aim of the study was to determine the activity of proteasomes and calpains and to reveal their association with VEGF, HIF-1α and NF-κΒ expressions in normal, primary and metastatic renal cell carcinoma (RCC) tissues.Methods. Ninety-three patients with renal cell carcinoma were included into the study. The expression levels of transcription factor and VEGF were measured using ELISA kits. The levels of proteasome subunits were measured by Western Blotting. Proteasome and calpain activities were determined using specific fluorogenic substrates.Results. We revealed inactivation of proteolysis in patients with kidney cancer. Disease advance was associated with a significant depression of cellular proteolysis and increase in transcription and growth factor levels in primary kidney cancer tissues. The proteolysis activation was found in metastatic tissues.Conclusions. Our results suggest that NF-κΒ, HIF-1α and VEGF transcription factors and intracellular proteolytic systems are involved in kidney cancer progression. |
| format | Article |
| id | doaj-art-5f50a4a18bdd4f428f2eee9417e080a9 |
| institution | Kabale University |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2018-09-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-5f50a4a18bdd4f428f2eee9417e080a92025-08-20T03:56:24ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682018-09-01174677410.21294/1814-4861-2018-17-4-67-74564IMPACT OF TRANSCRIPTION FACTORS, VEGF AND PROTEASES ON KIDNEY CANCER PROGRESSIONL. V. Spirina0I. V. Kondakova1E. A. Usynin2E. M. Slonimskaya3Z. A. Yurmazov4Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences Siberian State Medical UniversityCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences Siberian State Medical UniversityCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesIntroduction. The efficacy of anticancer treatment depends on biological factors of tumor.The aim of the study was to determine the activity of proteasomes and calpains and to reveal their association with VEGF, HIF-1α and NF-κΒ expressions in normal, primary and metastatic renal cell carcinoma (RCC) tissues.Methods. Ninety-three patients with renal cell carcinoma were included into the study. The expression levels of transcription factor and VEGF were measured using ELISA kits. The levels of proteasome subunits were measured by Western Blotting. Proteasome and calpain activities were determined using specific fluorogenic substrates.Results. We revealed inactivation of proteolysis in patients with kidney cancer. Disease advance was associated with a significant depression of cellular proteolysis and increase in transcription and growth factor levels in primary kidney cancer tissues. The proteolysis activation was found in metastatic tissues.Conclusions. Our results suggest that NF-κΒ, HIF-1α and VEGF transcription factors and intracellular proteolytic systems are involved in kidney cancer progression.https://www.siboncoj.ru/jour/article/view/818renal cell carcinomametastasisnf-квhif-1αvegf |
| spellingShingle | L. V. Spirina I. V. Kondakova E. A. Usynin E. M. Slonimskaya Z. A. Yurmazov IMPACT OF TRANSCRIPTION FACTORS, VEGF AND PROTEASES ON KIDNEY CANCER PROGRESSION Сибирский онкологический журнал renal cell carcinoma metastasis nf-кв hif-1α vegf |
| title | IMPACT OF TRANSCRIPTION FACTORS, VEGF AND PROTEASES ON KIDNEY CANCER PROGRESSION |
| title_full | IMPACT OF TRANSCRIPTION FACTORS, VEGF AND PROTEASES ON KIDNEY CANCER PROGRESSION |
| title_fullStr | IMPACT OF TRANSCRIPTION FACTORS, VEGF AND PROTEASES ON KIDNEY CANCER PROGRESSION |
| title_full_unstemmed | IMPACT OF TRANSCRIPTION FACTORS, VEGF AND PROTEASES ON KIDNEY CANCER PROGRESSION |
| title_short | IMPACT OF TRANSCRIPTION FACTORS, VEGF AND PROTEASES ON KIDNEY CANCER PROGRESSION |
| title_sort | impact of transcription factors vegf and proteases on kidney cancer progression |
| topic | renal cell carcinoma metastasis nf-кв hif-1α vegf |
| url | https://www.siboncoj.ru/jour/article/view/818 |
| work_keys_str_mv | AT lvspirina impactoftranscriptionfactorsvegfandproteasesonkidneycancerprogression AT ivkondakova impactoftranscriptionfactorsvegfandproteasesonkidneycancerprogression AT eausynin impactoftranscriptionfactorsvegfandproteasesonkidneycancerprogression AT emslonimskaya impactoftranscriptionfactorsvegfandproteasesonkidneycancerprogression AT zayurmazov impactoftranscriptionfactorsvegfandproteasesonkidneycancerprogression |