A STEDable BF<sub>2</sub>-Azadipyrromethene Fluorophore for Nuclear Membrane and Associated Endoplasmic Reticulum Imaging
The endoplasmic reticulum and the internal nuclear compartments are intrinsically connected through the nuclear membrane, pores and lamina. High resolution imaging of each of these cellular features concurrently remains a significant challenge. To that end we have developed a new molecular nuclear m...
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MDPI AG
2025-01-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/15/1/9 |
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| author | Anaïs C. Bourgès Massimiliano Garre Dan Wu Donal F. O’Shea |
| author_facet | Anaïs C. Bourgès Massimiliano Garre Dan Wu Donal F. O’Shea |
| author_sort | Anaïs C. Bourgès |
| collection | DOAJ |
| description | The endoplasmic reticulum and the internal nuclear compartments are intrinsically connected through the nuclear membrane, pores and lamina. High resolution imaging of each of these cellular features concurrently remains a significant challenge. To that end we have developed a new molecular nuclear membrane-endoplasmic reticulum (NM-ER) staining fluorophore with emission maxima at 650 nm. NM-ER is compatible with fixed and live cell imaging and stimulated emission depletion microscopy (STED) showing significant improvement in resolution when compared to comparable confocal laser scanning microscopy. The imaging versatility of NM-ER was illustrated through its compatible use with other fluorophores for co-imaging with DNA, nuclear pores and lamina allowing cellular abnormalities to be identified. NM-ER alone, or in use with other nuclear region labels could be an important tool for the investigation of nuclear transport and associated cellular processes. |
| format | Article |
| id | doaj-art-5f3e5d63d0eb419c840e55a1a9564893 |
| institution | Kabale University |
| issn | 2077-0375 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Membranes |
| spelling | doaj-art-5f3e5d63d0eb419c840e55a1a95648932025-01-24T13:41:00ZengMDPI AGMembranes2077-03752025-01-01151910.3390/membranes15010009A STEDable BF<sub>2</sub>-Azadipyrromethene Fluorophore for Nuclear Membrane and Associated Endoplasmic Reticulum ImagingAnaïs C. Bourgès0Massimiliano Garre1Dan Wu2Donal F. O’Shea3Department of Chemistry, RCSI, University of Medicine and Health Sciences, 123 St Stephen’s Green, D02 YN77 Dublin, IrelandDepartment of Chemistry, RCSI, University of Medicine and Health Sciences, 123 St Stephen’s Green, D02 YN77 Dublin, IrelandDepartment of Chemistry, RCSI, University of Medicine and Health Sciences, 123 St Stephen’s Green, D02 YN77 Dublin, IrelandDepartment of Chemistry, RCSI, University of Medicine and Health Sciences, 123 St Stephen’s Green, D02 YN77 Dublin, IrelandThe endoplasmic reticulum and the internal nuclear compartments are intrinsically connected through the nuclear membrane, pores and lamina. High resolution imaging of each of these cellular features concurrently remains a significant challenge. To that end we have developed a new molecular nuclear membrane-endoplasmic reticulum (NM-ER) staining fluorophore with emission maxima at 650 nm. NM-ER is compatible with fixed and live cell imaging and stimulated emission depletion microscopy (STED) showing significant improvement in resolution when compared to comparable confocal laser scanning microscopy. The imaging versatility of NM-ER was illustrated through its compatible use with other fluorophores for co-imaging with DNA, nuclear pores and lamina allowing cellular abnormalities to be identified. NM-ER alone, or in use with other nuclear region labels could be an important tool for the investigation of nuclear transport and associated cellular processes.https://www.mdpi.com/2077-0375/15/1/9nuclear membraneendoplasmic reticulumSTED microscopyfluorophoresBF<sub>2</sub>-azadipyrromethenenuclear pores |
| spellingShingle | Anaïs C. Bourgès Massimiliano Garre Dan Wu Donal F. O’Shea A STEDable BF<sub>2</sub>-Azadipyrromethene Fluorophore for Nuclear Membrane and Associated Endoplasmic Reticulum Imaging Membranes nuclear membrane endoplasmic reticulum STED microscopy fluorophores BF<sub>2</sub>-azadipyrromethene nuclear pores |
| title | A STEDable BF<sub>2</sub>-Azadipyrromethene Fluorophore for Nuclear Membrane and Associated Endoplasmic Reticulum Imaging |
| title_full | A STEDable BF<sub>2</sub>-Azadipyrromethene Fluorophore for Nuclear Membrane and Associated Endoplasmic Reticulum Imaging |
| title_fullStr | A STEDable BF<sub>2</sub>-Azadipyrromethene Fluorophore for Nuclear Membrane and Associated Endoplasmic Reticulum Imaging |
| title_full_unstemmed | A STEDable BF<sub>2</sub>-Azadipyrromethene Fluorophore for Nuclear Membrane and Associated Endoplasmic Reticulum Imaging |
| title_short | A STEDable BF<sub>2</sub>-Azadipyrromethene Fluorophore for Nuclear Membrane and Associated Endoplasmic Reticulum Imaging |
| title_sort | stedable bf sub 2 sub azadipyrromethene fluorophore for nuclear membrane and associated endoplasmic reticulum imaging |
| topic | nuclear membrane endoplasmic reticulum STED microscopy fluorophores BF<sub>2</sub>-azadipyrromethene nuclear pores |
| url | https://www.mdpi.com/2077-0375/15/1/9 |
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