Senescence Biomarkers CKAP4 and PTX3 Stratify Severe Kidney Disease Patients

Introduction: Cellular senescence is the irreversible growth arrest subsequent to oncogenic mutations, DNA damage, or metabolic insult. Senescence is associated with ageing and chronic age associated diseases such as cardiovascular disease and diabetes. The involvement of cellular senescence in acut...

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Main Authors: Sean McCallion, Thomas McLarnon, Eamonn Cooper, Andrew R. English, Steven Watterson, Melody El Chemaly, Cathy McGeough, Amanda Eakin, Tan Ahmed, Philip Gardiner, Adrian Pendleton, Gary Wright, Declan McGuigan, Maurice O’Kane, Aaron Peace, Ying Kuan, David S. Gibson, Paula L. McClean, Catriona Kelly, Victoria McGilligan, Elaine K. Murray, Frank McCarroll, Anthony J. Bjourson, Taranjit Singh Rai
Format: Article
Language:English
Published: MDPI AG 2024-09-01
Series:Cells
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Online Access:https://www.mdpi.com/2073-4409/13/19/1613
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author Sean McCallion
Thomas McLarnon
Eamonn Cooper
Andrew R. English
Steven Watterson
Melody El Chemaly
Cathy McGeough
Amanda Eakin
Tan Ahmed
Philip Gardiner
Adrian Pendleton
Gary Wright
Declan McGuigan
Maurice O’Kane
Aaron Peace
Ying Kuan
David S. Gibson
Paula L. McClean
Catriona Kelly
Victoria McGilligan
Elaine K. Murray
Frank McCarroll
Anthony J. Bjourson
Taranjit Singh Rai
author_facet Sean McCallion
Thomas McLarnon
Eamonn Cooper
Andrew R. English
Steven Watterson
Melody El Chemaly
Cathy McGeough
Amanda Eakin
Tan Ahmed
Philip Gardiner
Adrian Pendleton
Gary Wright
Declan McGuigan
Maurice O’Kane
Aaron Peace
Ying Kuan
David S. Gibson
Paula L. McClean
Catriona Kelly
Victoria McGilligan
Elaine K. Murray
Frank McCarroll
Anthony J. Bjourson
Taranjit Singh Rai
author_sort Sean McCallion
collection DOAJ
description Introduction: Cellular senescence is the irreversible growth arrest subsequent to oncogenic mutations, DNA damage, or metabolic insult. Senescence is associated with ageing and chronic age associated diseases such as cardiovascular disease and diabetes. The involvement of cellular senescence in acute kidney injury (AKI) and chronic kidney disease (CKD) is not fully understood. However, recent studies suggest that such patients have a higher-than-normal level of cellular senescence and accelerated ageing. Methods: This study aimed to discover key biomarkers of senescence in AKI and CKD patients compared to other chronic ageing diseases in controls using OLINK proteomics. Results: We show that senescence proteins CKAP4 (<i>p</i>-value < 0.0001) and PTX3 (<i>p</i>-value < 0.0001) are upregulated in AKI and CKD patients compared with controls with chronic diseases, suggesting the proteins may play a role in overall kidney disease development. Conclusions: CKAP4 was found to be differentially expressed in both AKI and CKD when compared to UHCs; hence, this biomarker could be a prognostic senescence biomarker of both AKI and CKD.
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spelling doaj-art-5f393bfa169e4c3a8f6b91c17a4e05b62025-08-20T01:47:42ZengMDPI AGCells2073-44092024-09-011319161310.3390/cells13191613Senescence Biomarkers CKAP4 and PTX3 Stratify Severe Kidney Disease PatientsSean McCallion0Thomas McLarnon1Eamonn Cooper2Andrew R. English3Steven Watterson4Melody El Chemaly5Cathy McGeough6Amanda Eakin7Tan Ahmed8Philip Gardiner9Adrian Pendleton10Gary Wright11Declan McGuigan12Maurice O’Kane13Aaron Peace14Ying Kuan15David S. Gibson16Paula L. McClean17Catriona Kelly18Victoria McGilligan19Elaine K. Murray20Frank McCarroll21Anthony J. Bjourson22Taranjit Singh Rai23Personalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKWestern Health and Social Care Trust (WHSCT), Altnagelvin Area Hospital, Londonderry BT47 6SB, UKBelfast Health and Social Care Trust (BHSCT), Belfast City Hospital, Belfast BT9 7AB, UKBelfast Health and Social Care Trust (BHSCT), Belfast City Hospital, Belfast BT9 7AB, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKWestern Health and Social Care Trust (WHSCT), Altnagelvin Area Hospital, Londonderry BT47 6SB, UKWestern Health and Social Care Trust (WHSCT), Altnagelvin Area Hospital, Londonderry BT47 6SB, UKWestern Health and Social Care Trust (WHSCT), Altnagelvin Area Hospital, Londonderry BT47 6SB, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKWestern Health and Social Care Trust (WHSCT), Altnagelvin Area Hospital, Londonderry BT47 6SB, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKPersonalised Medicine Centre, School of Medicine, Ulster University, Londonderry BT48 7JL, UKIntroduction: Cellular senescence is the irreversible growth arrest subsequent to oncogenic mutations, DNA damage, or metabolic insult. Senescence is associated with ageing and chronic age associated diseases such as cardiovascular disease and diabetes. The involvement of cellular senescence in acute kidney injury (AKI) and chronic kidney disease (CKD) is not fully understood. However, recent studies suggest that such patients have a higher-than-normal level of cellular senescence and accelerated ageing. Methods: This study aimed to discover key biomarkers of senescence in AKI and CKD patients compared to other chronic ageing diseases in controls using OLINK proteomics. Results: We show that senescence proteins CKAP4 (<i>p</i>-value < 0.0001) and PTX3 (<i>p</i>-value < 0.0001) are upregulated in AKI and CKD patients compared with controls with chronic diseases, suggesting the proteins may play a role in overall kidney disease development. Conclusions: CKAP4 was found to be differentially expressed in both AKI and CKD when compared to UHCs; hence, this biomarker could be a prognostic senescence biomarker of both AKI and CKD.https://www.mdpi.com/2073-4409/13/19/1613senescencechronic kidney diseaseacute kidney injurybiomarkermachine learning
spellingShingle Sean McCallion
Thomas McLarnon
Eamonn Cooper
Andrew R. English
Steven Watterson
Melody El Chemaly
Cathy McGeough
Amanda Eakin
Tan Ahmed
Philip Gardiner
Adrian Pendleton
Gary Wright
Declan McGuigan
Maurice O’Kane
Aaron Peace
Ying Kuan
David S. Gibson
Paula L. McClean
Catriona Kelly
Victoria McGilligan
Elaine K. Murray
Frank McCarroll
Anthony J. Bjourson
Taranjit Singh Rai
Senescence Biomarkers CKAP4 and PTX3 Stratify Severe Kidney Disease Patients
Cells
senescence
chronic kidney disease
acute kidney injury
biomarker
machine learning
title Senescence Biomarkers CKAP4 and PTX3 Stratify Severe Kidney Disease Patients
title_full Senescence Biomarkers CKAP4 and PTX3 Stratify Severe Kidney Disease Patients
title_fullStr Senescence Biomarkers CKAP4 and PTX3 Stratify Severe Kidney Disease Patients
title_full_unstemmed Senescence Biomarkers CKAP4 and PTX3 Stratify Severe Kidney Disease Patients
title_short Senescence Biomarkers CKAP4 and PTX3 Stratify Severe Kidney Disease Patients
title_sort senescence biomarkers ckap4 and ptx3 stratify severe kidney disease patients
topic senescence
chronic kidney disease
acute kidney injury
biomarker
machine learning
url https://www.mdpi.com/2073-4409/13/19/1613
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