Adipose-Derived Stem Cell Exosomes Promote Scar-Free Healing of Diabetic Wounds via miR-204-5p/TGF-β1/Smad Pathway
Numerous researches have demonstrated the therapeutic potential of adipose-derived stem cell exosomes (ADSC-Exos) in promoting wound healing. In this study, we aimed to investigate the impact of ADSC-Exos on diabetic wound fibroblasts and elucidate its possible mechanisms. CCK-8, Edu, cell scratch,...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/sci/6344844 |
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| author | Peijun Song Qiu Liang Xiuyu Ge Danlian Zhou Mei Yuan Weiwei Chu Jing Xu |
| author_facet | Peijun Song Qiu Liang Xiuyu Ge Danlian Zhou Mei Yuan Weiwei Chu Jing Xu |
| author_sort | Peijun Song |
| collection | DOAJ |
| description | Numerous researches have demonstrated the therapeutic potential of adipose-derived stem cell exosomes (ADSC-Exos) in promoting wound healing. In this study, we aimed to investigate the impact of ADSC-Exos on diabetic wound fibroblasts and elucidate its possible mechanisms. CCK-8, Edu, cell scratch, and Transwell tests were used to evaluate the function of ADSC-Exos on rat skin fibroblasts (RSFs) in high-glucose (HG) medium. The targeting effect of ADSC-Exo-derived microRNA (miRNA) and TGF-β1 was assessed using bioinformatic analysis and then confirmed with western blot and dual luciferase reporter assays. ADSC-Exos, miR-204-5p mimic, and anti-miR-204-5p mimic were used to stimulate RSFs, and the levels of TGF-β1/Smad pathway were analyzed by western blot. In vivo, digital photo and tissue section staining were used to evaluate the therapeutic effect of ADSC-Exos on diabetic wounds. The data showed that ADSC-Exos enhance the proliferation and migration of fibroblasts under HG conditions, reduce excessive myofibroblast differentiation and collagen deposition, and promote scarless healing of diabetic wounds. Additionally, miR-204-5p in ADSC-Exos targets TGF-β1 to inhibit p-Smad2/3, Col I, and alpha-smooth muscle actin (α-SMA), thereby reducing fibrosis. These findings suggest that ADSC-Exos have potential prospects for promoting diabetic wound healing. |
| format | Article |
| id | doaj-art-5f32088be2a646ddb6ffb1f88c7c19f5 |
| institution | DOAJ |
| issn | 1687-9678 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Stem Cells International |
| spelling | doaj-art-5f32088be2a646ddb6ffb1f88c7c19f52025-08-20T02:45:42ZengWileyStem Cells International1687-96782025-01-01202510.1155/sci/6344844Adipose-Derived Stem Cell Exosomes Promote Scar-Free Healing of Diabetic Wounds via miR-204-5p/TGF-β1/Smad PathwayPeijun Song0Qiu Liang1Xiuyu Ge2Danlian Zhou3Mei Yuan4Weiwei Chu5Jing Xu6Department of Plastic Surgery and BurnDepartment of Plastic SurgeryDepartment of Plastic Surgery and BurnDepartment of Plastic Surgery and BurnDepartment of Plastic Surgery and BurnDepartment of Plastic Surgery and BurnDepartment of Plastic Surgery and BurnNumerous researches have demonstrated the therapeutic potential of adipose-derived stem cell exosomes (ADSC-Exos) in promoting wound healing. In this study, we aimed to investigate the impact of ADSC-Exos on diabetic wound fibroblasts and elucidate its possible mechanisms. CCK-8, Edu, cell scratch, and Transwell tests were used to evaluate the function of ADSC-Exos on rat skin fibroblasts (RSFs) in high-glucose (HG) medium. The targeting effect of ADSC-Exo-derived microRNA (miRNA) and TGF-β1 was assessed using bioinformatic analysis and then confirmed with western blot and dual luciferase reporter assays. ADSC-Exos, miR-204-5p mimic, and anti-miR-204-5p mimic were used to stimulate RSFs, and the levels of TGF-β1/Smad pathway were analyzed by western blot. In vivo, digital photo and tissue section staining were used to evaluate the therapeutic effect of ADSC-Exos on diabetic wounds. The data showed that ADSC-Exos enhance the proliferation and migration of fibroblasts under HG conditions, reduce excessive myofibroblast differentiation and collagen deposition, and promote scarless healing of diabetic wounds. Additionally, miR-204-5p in ADSC-Exos targets TGF-β1 to inhibit p-Smad2/3, Col I, and alpha-smooth muscle actin (α-SMA), thereby reducing fibrosis. These findings suggest that ADSC-Exos have potential prospects for promoting diabetic wound healing.http://dx.doi.org/10.1155/sci/6344844 |
| spellingShingle | Peijun Song Qiu Liang Xiuyu Ge Danlian Zhou Mei Yuan Weiwei Chu Jing Xu Adipose-Derived Stem Cell Exosomes Promote Scar-Free Healing of Diabetic Wounds via miR-204-5p/TGF-β1/Smad Pathway Stem Cells International |
| title | Adipose-Derived Stem Cell Exosomes Promote Scar-Free Healing of Diabetic Wounds via miR-204-5p/TGF-β1/Smad Pathway |
| title_full | Adipose-Derived Stem Cell Exosomes Promote Scar-Free Healing of Diabetic Wounds via miR-204-5p/TGF-β1/Smad Pathway |
| title_fullStr | Adipose-Derived Stem Cell Exosomes Promote Scar-Free Healing of Diabetic Wounds via miR-204-5p/TGF-β1/Smad Pathway |
| title_full_unstemmed | Adipose-Derived Stem Cell Exosomes Promote Scar-Free Healing of Diabetic Wounds via miR-204-5p/TGF-β1/Smad Pathway |
| title_short | Adipose-Derived Stem Cell Exosomes Promote Scar-Free Healing of Diabetic Wounds via miR-204-5p/TGF-β1/Smad Pathway |
| title_sort | adipose derived stem cell exosomes promote scar free healing of diabetic wounds via mir 204 5p tgf β1 smad pathway |
| url | http://dx.doi.org/10.1155/sci/6344844 |
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