Genome-wide association study of the antibody response to <i>Corynebacterium pseudotuberculosis</i> in sheep
<p>Caseous lymphadenitis (CLA) is a chronic and highly contagious disease that is widespread among sheep and goats and adversely affects animal welfare and production. This study aimed to reveal the host genomic influences on <i>Corynebacterium pseudotuberculosis</i> antibody level...
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| Format: | Article |
| Language: | English |
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Copernicus Publications
2025-02-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/68/109/2025/aab-68-109-2025.pdf |
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| author | J. Kyselová L. Tichý J. Marková A. Gurgul Z. Sztankóová K. Vališ S. Šlosárková K. Kavanová M. Beinhauerová T. Szmatola T. Szmatola |
| author_facet | J. Kyselová L. Tichý J. Marková A. Gurgul Z. Sztankóová K. Vališ S. Šlosárková K. Kavanová M. Beinhauerová T. Szmatola T. Szmatola |
| author_sort | J. Kyselová |
| collection | DOAJ |
| description | <p>Caseous lymphadenitis (CLA) is a chronic and highly contagious disease that is widespread among sheep and goats and adversely affects animal welfare and production. This study aimed to reveal the host genomic influences on <i>Corynebacterium pseudotuberculosis</i> antibody levels as indicators of disease susceptibility or resistance, thereby potentially identifying genetic markers associated with these traits. Blood samples were collected from 321 sheep from four large Czech sheep herds. Animals of the Suffolk breed, ranging in age from 2 to 8 years, were sampled regularly once a year for 4 years. The sheep were classified into healthy and diseased groups based on two different commercial enzyme-linked immunosorbent assay (ELISA) serological antigen tests. Genomic DNA was genotyped with the GeneSeek<sup>®</sup> Genomic Profiler™ Ovine 50 K, and 41 301 markers were used for the genome-wide association analysis (GWAS). A case–control GWAS including 143 seropositive and 178 seronegative sheep was conducted to assess the relationship between the sheep genome and the antibody response to a phospholipase D antigen of <i>C. pseudotuberculosis</i> using GCTA software. The study revealed two suggestive SNPs (single-nucleotide polymorphisms) on chromosomes 11 and 20, with the most significant SNP in the first exon of the <i>TRIM16</i> (tripartite-motif-containing 16) gene. By analysing genomic alterations and their context between positive and negative animals, including a gene ontology (GO) analysis of genes within <span class="inline-formula">±</span> 500 kb regions of the suggestive SNPs, we identified genes and immune-related processes and pathways potentially influencing CLA susceptibility. These include antigen processing and presentation of exogenous peptides via major histocompatibility complex (MHC) class I and II, Th17 mucosal cell differentiation, cellular autophagy, and phagosome-related mechanisms. This research provides insights into the genetic basis of the antibody response to <i>C. pseudotuberculosis</i>, identifying suggestive associations and underlying biological mechanisms that could guide future breeding and genetic strategies for improving resistance to CLA in sheep.</p> |
| format | Article |
| id | doaj-art-5f30ba40a6d649ffb74e77ecb68a76b6 |
| institution | Kabale University |
| issn | 0003-9438 2363-9822 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Archives Animal Breeding |
| spelling | doaj-art-5f30ba40a6d649ffb74e77ecb68a76b62025-02-11T10:45:37ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222025-02-016810912410.5194/aab-68-109-2025Genome-wide association study of the antibody response to <i>Corynebacterium pseudotuberculosis</i> in sheepJ. Kyselová0L. Tichý1J. Marková2A. Gurgul3Z. Sztankóová4K. Vališ5S. Šlosárková6K. Kavanová7M. Beinhauerová8T. Szmatola9T. Szmatola10Department of Genetics and Breeding of Farm Animals, Institute of Animal Science, 104 00 Prague, Czech RepublicDepartment of Genetics and Breeding of Farm Animals, Institute of Animal Science, 104 00 Prague, Czech RepublicDepartment of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, 621 00 Brno, Czech RepublicCentre for Experimental and Innovative Medicine, University of Agriculture in Krakow, 30-248 Kraków, PolandDepartment of Genetics and Breeding of Farm Animals, Institute of Animal Science, 104 00 Prague, Czech RepublicDepartment of Genetics and Breeding of Farm Animals, Institute of Animal Science, 104 00 Prague, Czech RepublicDepartment of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, 621 00 Brno, Czech RepublicDepartment of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, 621 00 Brno, Czech RepublicDepartment of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, 621 00 Brno, Czech RepublicCentre for Experimental and Innovative Medicine, University of Agriculture in Krakow, 30-248 Kraków, PolandDepartment of Animal Molecular Biology, National Research Institute of Animal Production, 32-083 Balice, Poland<p>Caseous lymphadenitis (CLA) is a chronic and highly contagious disease that is widespread among sheep and goats and adversely affects animal welfare and production. This study aimed to reveal the host genomic influences on <i>Corynebacterium pseudotuberculosis</i> antibody levels as indicators of disease susceptibility or resistance, thereby potentially identifying genetic markers associated with these traits. Blood samples were collected from 321 sheep from four large Czech sheep herds. Animals of the Suffolk breed, ranging in age from 2 to 8 years, were sampled regularly once a year for 4 years. The sheep were classified into healthy and diseased groups based on two different commercial enzyme-linked immunosorbent assay (ELISA) serological antigen tests. Genomic DNA was genotyped with the GeneSeek<sup>®</sup> Genomic Profiler™ Ovine 50 K, and 41 301 markers were used for the genome-wide association analysis (GWAS). A case–control GWAS including 143 seropositive and 178 seronegative sheep was conducted to assess the relationship between the sheep genome and the antibody response to a phospholipase D antigen of <i>C. pseudotuberculosis</i> using GCTA software. The study revealed two suggestive SNPs (single-nucleotide polymorphisms) on chromosomes 11 and 20, with the most significant SNP in the first exon of the <i>TRIM16</i> (tripartite-motif-containing 16) gene. By analysing genomic alterations and their context between positive and negative animals, including a gene ontology (GO) analysis of genes within <span class="inline-formula">±</span> 500 kb regions of the suggestive SNPs, we identified genes and immune-related processes and pathways potentially influencing CLA susceptibility. These include antigen processing and presentation of exogenous peptides via major histocompatibility complex (MHC) class I and II, Th17 mucosal cell differentiation, cellular autophagy, and phagosome-related mechanisms. This research provides insights into the genetic basis of the antibody response to <i>C. pseudotuberculosis</i>, identifying suggestive associations and underlying biological mechanisms that could guide future breeding and genetic strategies for improving resistance to CLA in sheep.</p>https://aab.copernicus.org/articles/68/109/2025/aab-68-109-2025.pdf |
| spellingShingle | J. Kyselová L. Tichý J. Marková A. Gurgul Z. Sztankóová K. Vališ S. Šlosárková K. Kavanová M. Beinhauerová T. Szmatola T. Szmatola Genome-wide association study of the antibody response to <i>Corynebacterium pseudotuberculosis</i> in sheep Archives Animal Breeding |
| title | Genome-wide association study of the antibody response to <i>Corynebacterium pseudotuberculosis</i> in sheep |
| title_full | Genome-wide association study of the antibody response to <i>Corynebacterium pseudotuberculosis</i> in sheep |
| title_fullStr | Genome-wide association study of the antibody response to <i>Corynebacterium pseudotuberculosis</i> in sheep |
| title_full_unstemmed | Genome-wide association study of the antibody response to <i>Corynebacterium pseudotuberculosis</i> in sheep |
| title_short | Genome-wide association study of the antibody response to <i>Corynebacterium pseudotuberculosis</i> in sheep |
| title_sort | genome wide association study of the antibody response to i corynebacterium pseudotuberculosis i in sheep |
| url | https://aab.copernicus.org/articles/68/109/2025/aab-68-109-2025.pdf |
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