CRISPR/Cas9 recombineering‐mediated deep mutational scanning of essential genes in Escherichia coli
Abstract Deep mutational scanning can provide significant insights into the function of essential genes in bacteria. Here, we developed a high‐throughput method for mutating essential genes of Escherichia coli in their native genetic context. We used Cas9‐mediated recombineering to introduce a libra...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Nature
2020-03-01
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| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.15252/msb.20199265 |
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