Expression of Progenitor Cell Markers in the Glial-Like Cells of Epiretinal Membranes of Different Origins
Purpose. To investigate the expression of progenitor cell markers (Sox2, Nestin, and Pax2) in idiopathic epiretinal membranes (iERMs) and nonidiopathic epiretinal membranes (niERMs) in relation to glial cell marker expression. Methods. ERMs were obtained from patients with iERMs and niERMs of differ...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2018/7096326 |
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| author | Xiaohe Yan Pål Andresen Xhevat Lumi Qingshan Chen Goran Petrovski |
| author_facet | Xiaohe Yan Pål Andresen Xhevat Lumi Qingshan Chen Goran Petrovski |
| author_sort | Xiaohe Yan |
| collection | DOAJ |
| description | Purpose. To investigate the expression of progenitor cell markers (Sox2, Nestin, and Pax2) in idiopathic epiretinal membranes (iERMs) and nonidiopathic epiretinal membranes (niERMs) in relation to glial cell marker expression. Methods. ERMs were obtained from patients with iERMs and niERMs of different origins: proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR), and uveitis. The membranes were studied by flat-mount or sectional immunohistochemistry for expression of progenitor cell markers as well as glial (GFAP) and proliferation (Ki-67) markers. Results. Cells in the ERMs express strong GFAP, with strong Pax2 expression in the cell nuclei. Some of the GFAP-positive glial cells in all epiretinal membrane types colocalized with Sox2, Pax2, and Nestin. NiERMs are much more cellular than iERMs. Glial cells are more densely packed in all analyzed niERMs, whereas glial cells with long branches are found in the internal limiting membrane parts and the iERMs, which appear to form a local network by their processes. Conclusion. The GFAP-positive glial cells in ERMs are not pure glial cells, and some of them express progenitor cell markers, which indicate that these cells may have potential for self-renewal and differentiation into more glial or neuroglial type of cells. |
| format | Article |
| id | doaj-art-5f2508a2df7b4b09893a426e98d4edae |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-5f2508a2df7b4b09893a426e98d4edae2025-08-20T03:34:22ZengWileyJournal of Ophthalmology2090-004X2090-00582018-01-01201810.1155/2018/70963267096326Expression of Progenitor Cell Markers in the Glial-Like Cells of Epiretinal Membranes of Different OriginsXiaohe Yan0Pål Andresen1Xhevat Lumi2Qingshan Chen3Goran Petrovski4Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, ChinaCenter for Eye Research, Department of Ophthalmology, Oslo University Hospital and University of Oslo, Kirkeveien 166, N-0407 Oslo, NorwayDepartment of Ophthalmology, University Medical Centre Ljubljana, Ljubljana, SloveniaShenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, ChinaCenter for Eye Research, Department of Ophthalmology, Oslo University Hospital and University of Oslo, Kirkeveien 166, N-0407 Oslo, NorwayPurpose. To investigate the expression of progenitor cell markers (Sox2, Nestin, and Pax2) in idiopathic epiretinal membranes (iERMs) and nonidiopathic epiretinal membranes (niERMs) in relation to glial cell marker expression. Methods. ERMs were obtained from patients with iERMs and niERMs of different origins: proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR), and uveitis. The membranes were studied by flat-mount or sectional immunohistochemistry for expression of progenitor cell markers as well as glial (GFAP) and proliferation (Ki-67) markers. Results. Cells in the ERMs express strong GFAP, with strong Pax2 expression in the cell nuclei. Some of the GFAP-positive glial cells in all epiretinal membrane types colocalized with Sox2, Pax2, and Nestin. NiERMs are much more cellular than iERMs. Glial cells are more densely packed in all analyzed niERMs, whereas glial cells with long branches are found in the internal limiting membrane parts and the iERMs, which appear to form a local network by their processes. Conclusion. The GFAP-positive glial cells in ERMs are not pure glial cells, and some of them express progenitor cell markers, which indicate that these cells may have potential for self-renewal and differentiation into more glial or neuroglial type of cells.http://dx.doi.org/10.1155/2018/7096326 |
| spellingShingle | Xiaohe Yan Pål Andresen Xhevat Lumi Qingshan Chen Goran Petrovski Expression of Progenitor Cell Markers in the Glial-Like Cells of Epiretinal Membranes of Different Origins Journal of Ophthalmology |
| title | Expression of Progenitor Cell Markers in the Glial-Like Cells of Epiretinal Membranes of Different Origins |
| title_full | Expression of Progenitor Cell Markers in the Glial-Like Cells of Epiretinal Membranes of Different Origins |
| title_fullStr | Expression of Progenitor Cell Markers in the Glial-Like Cells of Epiretinal Membranes of Different Origins |
| title_full_unstemmed | Expression of Progenitor Cell Markers in the Glial-Like Cells of Epiretinal Membranes of Different Origins |
| title_short | Expression of Progenitor Cell Markers in the Glial-Like Cells of Epiretinal Membranes of Different Origins |
| title_sort | expression of progenitor cell markers in the glial like cells of epiretinal membranes of different origins |
| url | http://dx.doi.org/10.1155/2018/7096326 |
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