Recurrence of early stage colon cancer predicted by expression pattern of circulating microRNAs.
Systemic treatment of patients with early-stage cancers attempts to eradicate occult metastatic disease to prevent recurrence and increased morbidity. However, prediction of recurrence from an analysis of the primary tumor is limited because disseminated cancer cells only represent a small subset of...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0084686 |
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| author | Narayan Shivapurkar Louis M Weiner John L Marshall Subha Madhavan Anne Deslattes Mays Hartmut Juhl Anton Wellstein |
| author_facet | Narayan Shivapurkar Louis M Weiner John L Marshall Subha Madhavan Anne Deslattes Mays Hartmut Juhl Anton Wellstein |
| author_sort | Narayan Shivapurkar |
| collection | DOAJ |
| description | Systemic treatment of patients with early-stage cancers attempts to eradicate occult metastatic disease to prevent recurrence and increased morbidity. However, prediction of recurrence from an analysis of the primary tumor is limited because disseminated cancer cells only represent a small subset of the primary lesion. Here we analyze the expression of circulating microRNAs (miRs) in serum obtained pre-surgically from patients with early stage colorectal cancers. Groups of five patients with and without disease recurrence were used to identify an informative panel of circulating miRs using quantitative PCR of genome-wide miR expression as well as a set of published candidate miRs. A panel of six informative miRs (miR-15a, mir-103, miR-148a, miR-320a, miR-451, miR-596) was derived from this analysis and evaluated in a separate validation set of thirty patients. Hierarchical clustering of the expression levels of these six circulating miRs and Kaplan-Meier analysis showed that the risk of disease recurrence of early stage colon cancer can be predicted by this panel of miRs that are measurable in the circulation at the time of diagnosis (P = 0.0026; Hazard Ratio 5.4; 95% CI of 1.9 to 15). |
| format | Article |
| id | doaj-art-5f1773bceda8472fbd337fdf6a1a1f68 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-5f1773bceda8472fbd337fdf6a1a1f682025-08-20T02:22:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8468610.1371/journal.pone.0084686Recurrence of early stage colon cancer predicted by expression pattern of circulating microRNAs.Narayan ShivapurkarLouis M WeinerJohn L MarshallSubha MadhavanAnne Deslattes MaysHartmut JuhlAnton WellsteinSystemic treatment of patients with early-stage cancers attempts to eradicate occult metastatic disease to prevent recurrence and increased morbidity. However, prediction of recurrence from an analysis of the primary tumor is limited because disseminated cancer cells only represent a small subset of the primary lesion. Here we analyze the expression of circulating microRNAs (miRs) in serum obtained pre-surgically from patients with early stage colorectal cancers. Groups of five patients with and without disease recurrence were used to identify an informative panel of circulating miRs using quantitative PCR of genome-wide miR expression as well as a set of published candidate miRs. A panel of six informative miRs (miR-15a, mir-103, miR-148a, miR-320a, miR-451, miR-596) was derived from this analysis and evaluated in a separate validation set of thirty patients. Hierarchical clustering of the expression levels of these six circulating miRs and Kaplan-Meier analysis showed that the risk of disease recurrence of early stage colon cancer can be predicted by this panel of miRs that are measurable in the circulation at the time of diagnosis (P = 0.0026; Hazard Ratio 5.4; 95% CI of 1.9 to 15).https://doi.org/10.1371/journal.pone.0084686 |
| spellingShingle | Narayan Shivapurkar Louis M Weiner John L Marshall Subha Madhavan Anne Deslattes Mays Hartmut Juhl Anton Wellstein Recurrence of early stage colon cancer predicted by expression pattern of circulating microRNAs. PLoS ONE |
| title | Recurrence of early stage colon cancer predicted by expression pattern of circulating microRNAs. |
| title_full | Recurrence of early stage colon cancer predicted by expression pattern of circulating microRNAs. |
| title_fullStr | Recurrence of early stage colon cancer predicted by expression pattern of circulating microRNAs. |
| title_full_unstemmed | Recurrence of early stage colon cancer predicted by expression pattern of circulating microRNAs. |
| title_short | Recurrence of early stage colon cancer predicted by expression pattern of circulating microRNAs. |
| title_sort | recurrence of early stage colon cancer predicted by expression pattern of circulating micrornas |
| url | https://doi.org/10.1371/journal.pone.0084686 |
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