Study of the mechanisms of colistin heteroresistance in a strain of Enterobacter cloacae by random mutagenesis
Objective: The Enterobacter cloacae complex belongs to a group in which colistin resistance is not well documented due to their frequent heteroresistance. Methods: We investigated the molecular basis of colistin heteroresistance using genome analysis and random mutagenesis in a strain of E. cloacae....
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524004405 |
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| Summary: | Objective: The Enterobacter cloacae complex belongs to a group in which colistin resistance is not well documented due to their frequent heteroresistance. Methods: We investigated the molecular basis of colistin heteroresistance using genome analysis and random mutagenesis in a strain of E. cloacae. Results: The arnA gene was truncated in the six colistin susceptible mutants we obtained. However, the mcr-9 gene was present in these mutants suggesting this gene is not responsible for colistin resistance. Conclusions: Previous studies have highlighted the role of the two-component system PhoPQ and its negative regulator mgrB or the arnBCADTEF operon. Our results confirm the crucial role of this operon. |
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| ISSN: | 2213-7165 |