Maternal Supplementation with <i>Lacticaseibacillus rhamnosus</i> GG Improves Glucose Tolerance and Modulates the Intestinal Microbiota of Offspring
Introduction: Consuming hypercaloric diets during pregnancy induces metabolic, immune, and maternal intestinal dysbiosis disorders. These conditions are transferred to the offspring through the placenta and breastfeeding, increasing susceptibility to metabolic diseases. We investigated the effect of...
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2024-12-01
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| author | Dayane Correia Gomes José Enrique Meza Alvarado Jesus Alejandro Zamora Briseño Cynthia Cano Sarmiento Alberto Camacho Morales Rubi Viveros Contreras |
| author_facet | Dayane Correia Gomes José Enrique Meza Alvarado Jesus Alejandro Zamora Briseño Cynthia Cano Sarmiento Alberto Camacho Morales Rubi Viveros Contreras |
| author_sort | Dayane Correia Gomes |
| collection | DOAJ |
| description | Introduction: Consuming hypercaloric diets during pregnancy induces metabolic, immune, and maternal intestinal dysbiosis disorders. These conditions are transferred to the offspring through the placenta and breastfeeding, increasing susceptibility to metabolic diseases. We investigated the effect of <i>L. rhamnosus</i> GG supplementation on offspring maternally programmed with a hypercaloric diet. Methods: Our study involved sixteen female Wistar rats aged ten weeks, which were divided into four groups based on their diets: control (Ctrl), cafeteria (CAF), control + probiotic (PRO), and cafeteria + probiotic (CPRO). The control + probiotic and cafeteria + probiotic groups received a daily oral administration of 250 μL of <i>L. rhamnosus</i> GG cell suspension (equivalent to 10<sup>9</sup> UFC) for nine weeks. The body weight of the animals was recorded weekly, and their food intake was monitored every 24 h. An oral glucose tolerance test was conducted on the offspring at seven weeks of age. At the ninth week of age, animals were euthanized, and blood, tissues, and organs were collected. Results: Maternal supplementation with <i>L. rhamnosus</i> GG decreased food intake and the average birth weight, improved glucose sensitivity, and lowered the levels of LDL, cholesterol, triglycerides, and mesenteric adipose tissue in offspring compared with the control and cafeteria groups. Conclusions: Our findings indicate that supplementing with LGG during maternal programming could protect offspring from metabolic disruptions caused by a hypercaloric maternal diet. |
| format | Article |
| id | doaj-art-5f0b6fd7627b4ad7aeea5b49ccf976c3 |
| institution | DOAJ |
| issn | 2079-9721 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Diseases |
| spelling | doaj-art-5f0b6fd7627b4ad7aeea5b49ccf976c32025-08-20T02:57:12ZengMDPI AGDiseases2079-97212024-12-01121231210.3390/diseases12120312Maternal Supplementation with <i>Lacticaseibacillus rhamnosus</i> GG Improves Glucose Tolerance and Modulates the Intestinal Microbiota of OffspringDayane Correia Gomes0José Enrique Meza Alvarado1Jesus Alejandro Zamora Briseño2Cynthia Cano Sarmiento3Alberto Camacho Morales4Rubi Viveros Contreras5Centro de Investigaciones Biomédicas, Doctorado en Ciencias Biomédicas, Universidad Veracruzana, Xalapa 91190, MexicoCentro de Investigaciones Biomédicas, Doctorado en Ciencias Biomédicas, Universidad Veracruzana, Xalapa 91190, MexicoAdvanced Molecular Studies Network, Campus III, Institute of Ecology A. C., Xalapa 91612, MexicoFood Research and Development Unit, Technological Institute of Veracruz, National Institute of Technology of Mexico, M.A. de Quevedo 2779, Veracruz 91897, MexicoFaculty of Medicine, Department of Biochemistry and Molecular Medicine, Autonomous University of Nuevo León, Monterrey 66455, MexicoCentro de Investigaciones Biomédicas, Doctorado en Ciencias Biomédicas, Universidad Veracruzana, Xalapa 91190, MexicoIntroduction: Consuming hypercaloric diets during pregnancy induces metabolic, immune, and maternal intestinal dysbiosis disorders. These conditions are transferred to the offspring through the placenta and breastfeeding, increasing susceptibility to metabolic diseases. We investigated the effect of <i>L. rhamnosus</i> GG supplementation on offspring maternally programmed with a hypercaloric diet. Methods: Our study involved sixteen female Wistar rats aged ten weeks, which were divided into four groups based on their diets: control (Ctrl), cafeteria (CAF), control + probiotic (PRO), and cafeteria + probiotic (CPRO). The control + probiotic and cafeteria + probiotic groups received a daily oral administration of 250 μL of <i>L. rhamnosus</i> GG cell suspension (equivalent to 10<sup>9</sup> UFC) for nine weeks. The body weight of the animals was recorded weekly, and their food intake was monitored every 24 h. An oral glucose tolerance test was conducted on the offspring at seven weeks of age. At the ninth week of age, animals were euthanized, and blood, tissues, and organs were collected. Results: Maternal supplementation with <i>L. rhamnosus</i> GG decreased food intake and the average birth weight, improved glucose sensitivity, and lowered the levels of LDL, cholesterol, triglycerides, and mesenteric adipose tissue in offspring compared with the control and cafeteria groups. Conclusions: Our findings indicate that supplementing with LGG during maternal programming could protect offspring from metabolic disruptions caused by a hypercaloric maternal diet.https://www.mdpi.com/2079-9721/12/12/312<i>Lacticaseibacillus rhamnosus</i>maternal programmingmetabolic syndromeglucose intolerancehigh-fat diet |
| spellingShingle | Dayane Correia Gomes José Enrique Meza Alvarado Jesus Alejandro Zamora Briseño Cynthia Cano Sarmiento Alberto Camacho Morales Rubi Viveros Contreras Maternal Supplementation with <i>Lacticaseibacillus rhamnosus</i> GG Improves Glucose Tolerance and Modulates the Intestinal Microbiota of Offspring Diseases <i>Lacticaseibacillus rhamnosus</i> maternal programming metabolic syndrome glucose intolerance high-fat diet |
| title | Maternal Supplementation with <i>Lacticaseibacillus rhamnosus</i> GG Improves Glucose Tolerance and Modulates the Intestinal Microbiota of Offspring |
| title_full | Maternal Supplementation with <i>Lacticaseibacillus rhamnosus</i> GG Improves Glucose Tolerance and Modulates the Intestinal Microbiota of Offspring |
| title_fullStr | Maternal Supplementation with <i>Lacticaseibacillus rhamnosus</i> GG Improves Glucose Tolerance and Modulates the Intestinal Microbiota of Offspring |
| title_full_unstemmed | Maternal Supplementation with <i>Lacticaseibacillus rhamnosus</i> GG Improves Glucose Tolerance and Modulates the Intestinal Microbiota of Offspring |
| title_short | Maternal Supplementation with <i>Lacticaseibacillus rhamnosus</i> GG Improves Glucose Tolerance and Modulates the Intestinal Microbiota of Offspring |
| title_sort | maternal supplementation with i lacticaseibacillus rhamnosus i gg improves glucose tolerance and modulates the intestinal microbiota of offspring |
| topic | <i>Lacticaseibacillus rhamnosus</i> maternal programming metabolic syndrome glucose intolerance high-fat diet |
| url | https://www.mdpi.com/2079-9721/12/12/312 |
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