The characteristics of intestinal microflora in infants with rotavirus enteritis, changes in microflora before and after treatment and their clinical values
Abstract Rotavirus (RV) is a leading pathogen causing diarrhea in children. In this study, a total of 51 fecal samples from children with RV enteritis, 29 post-treatment fecal samples, and 38 fecal samples from age-matched healthy controls were collected. Microbial DNA was isolated from the samples...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-88312-w |
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| author | Qiyan Yan Yuanyuan Chen E-Bin Gao Yanbo Lu Junhua Wu Haiyan Qiu |
| author_facet | Qiyan Yan Yuanyuan Chen E-Bin Gao Yanbo Lu Junhua Wu Haiyan Qiu |
| author_sort | Qiyan Yan |
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| description | Abstract Rotavirus (RV) is a leading pathogen causing diarrhea in children. In this study, a total of 51 fecal samples from children with RV enteritis, 29 post-treatment fecal samples, and 38 fecal samples from age-matched healthy controls were collected. Microbial DNA was isolated from the samples followed by high throughput Illumina sequencing targeting 16 S rRNA gene. Compared to the healthy group, the RV-infected group exhibited reduced microbial diversity. Both groups shared Firmicutes as the dominant phylum. Additionally, the abundance of Proteobacteria increased significantly in the RV-infected group. At the genus level, among the top 50 most abundant genera, 34 showed significant differences, with these differential genera correlating with certain clinical indicators such as dehydration levels and C-reactive protein (CRP). Notably, there were no significant differences in the microbiota before and after treatment in RV-infected children. Only 8.82% (3/34) of the differential genera in the post-treatment group showed a recovery trend towards the healthy state. This study enhances the understanding of how RV infection alters the gut microbiota structure in children and provides a scientific basis for improving clinical diagnosis and treatment strategies. |
| format | Article |
| id | doaj-art-5f092e99239046df94d8b63aee5a6655 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-5f092e99239046df94d8b63aee5a66552025-02-09T12:36:35ZengNature PortfolioScientific Reports2045-23222025-02-0115111010.1038/s41598-025-88312-wThe characteristics of intestinal microflora in infants with rotavirus enteritis, changes in microflora before and after treatment and their clinical valuesQiyan Yan0Yuanyuan Chen1E-Bin Gao2Yanbo Lu3Junhua Wu4Haiyan Qiu5Department of Pediatrics, The Affiliated Women and Children’s Hospital of Ningbo UniversityDepartment of Pediatrics, Jinhua Women’s and Children’s HospitalSchool of Life Sciences, Jiangsu UniversityDepartment of Pediatrics, The Affiliated Women and Children’s Hospital of Ningbo UniversityDepartment of Pediatrics, The Affiliated Women and Children’s Hospital of Ningbo UniversityDepartment of Pediatrics, The Affiliated Women and Children’s Hospital of Ningbo UniversityAbstract Rotavirus (RV) is a leading pathogen causing diarrhea in children. In this study, a total of 51 fecal samples from children with RV enteritis, 29 post-treatment fecal samples, and 38 fecal samples from age-matched healthy controls were collected. Microbial DNA was isolated from the samples followed by high throughput Illumina sequencing targeting 16 S rRNA gene. Compared to the healthy group, the RV-infected group exhibited reduced microbial diversity. Both groups shared Firmicutes as the dominant phylum. Additionally, the abundance of Proteobacteria increased significantly in the RV-infected group. At the genus level, among the top 50 most abundant genera, 34 showed significant differences, with these differential genera correlating with certain clinical indicators such as dehydration levels and C-reactive protein (CRP). Notably, there were no significant differences in the microbiota before and after treatment in RV-infected children. Only 8.82% (3/34) of the differential genera in the post-treatment group showed a recovery trend towards the healthy state. This study enhances the understanding of how RV infection alters the gut microbiota structure in children and provides a scientific basis for improving clinical diagnosis and treatment strategies.https://doi.org/10.1038/s41598-025-88312-wInfants and young childrenRotavirus enteritisIntestinal flora16S rRNAClinical value |
| spellingShingle | Qiyan Yan Yuanyuan Chen E-Bin Gao Yanbo Lu Junhua Wu Haiyan Qiu The characteristics of intestinal microflora in infants with rotavirus enteritis, changes in microflora before and after treatment and their clinical values Scientific Reports Infants and young children Rotavirus enteritis Intestinal flora 16S rRNA Clinical value |
| title | The characteristics of intestinal microflora in infants with rotavirus enteritis, changes in microflora before and after treatment and their clinical values |
| title_full | The characteristics of intestinal microflora in infants with rotavirus enteritis, changes in microflora before and after treatment and their clinical values |
| title_fullStr | The characteristics of intestinal microflora in infants with rotavirus enteritis, changes in microflora before and after treatment and their clinical values |
| title_full_unstemmed | The characteristics of intestinal microflora in infants with rotavirus enteritis, changes in microflora before and after treatment and their clinical values |
| title_short | The characteristics of intestinal microflora in infants with rotavirus enteritis, changes in microflora before and after treatment and their clinical values |
| title_sort | characteristics of intestinal microflora in infants with rotavirus enteritis changes in microflora before and after treatment and their clinical values |
| topic | Infants and young children Rotavirus enteritis Intestinal flora 16S rRNA Clinical value |
| url | https://doi.org/10.1038/s41598-025-88312-w |
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