AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing
Abstract Current methods for producing cardiomyocytes from human induced pluripotent stem cells (hiPSCs) using 2D monolayer differentiation are often hampered by batch-to-batch variability and inefficient purification processes. Here, we introduce CM-AI, a novel artificial intelligence-guided laser...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-05-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-08162-0 |
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| author | Prakaimuk Saraithong Peyton Krajcarski Yukako Kusaka Moe Yamada Junichi Matsumoto Hailey Cunningham Sama Salih Darby Jones Devika Baddhan Christian Hausner Justus Anumonwo Anthony Rosenzweig Mary M. Navarro Luis Villa Diaz Joseph Criscione Deok-Ho Kim Todd J. Herron |
| author_facet | Prakaimuk Saraithong Peyton Krajcarski Yukako Kusaka Moe Yamada Junichi Matsumoto Hailey Cunningham Sama Salih Darby Jones Devika Baddhan Christian Hausner Justus Anumonwo Anthony Rosenzweig Mary M. Navarro Luis Villa Diaz Joseph Criscione Deok-Ho Kim Todd J. Herron |
| author_sort | Prakaimuk Saraithong |
| collection | DOAJ |
| description | Abstract Current methods for producing cardiomyocytes from human induced pluripotent stem cells (hiPSCs) using 2D monolayer differentiation are often hampered by batch-to-batch variability and inefficient purification processes. Here, we introduce CM-AI, a novel artificial intelligence-guided laser cell processing platform designed for rapid, label-free purification of hiPSC-derived cardiomyocytes (hiPSC-CMs). This approach significantly reduces processing time without the need for chronic metabolic selection or antibody-based sorting. By integrating real-time cellular morphology analysis and targeted laser ablation, CM-AI selectively removes non-cardiomyocyte populations with high precision. This streamlined process preserves cardiomyocyte viability and function, offering a scalable and efficient solution for cardiac regenerative medicine, disease modeling, and drug discovery. |
| format | Article |
| id | doaj-art-5f067b4f67464d5d95228ffad96ec5db |
| institution | OA Journals |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-5f067b4f67464d5d95228ffad96ec5db2025-08-20T02:25:12ZengNature PortfolioCommunications Biology2399-36422025-05-018111010.1038/s42003-025-08162-0AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturingPrakaimuk Saraithong0Peyton Krajcarski1Yukako Kusaka2Moe Yamada3Junichi Matsumoto4Hailey Cunningham5Sama Salih6Darby Jones7Devika Baddhan8Christian Hausner9Justus Anumonwo10Anthony Rosenzweig11Mary M. Navarro12Luis Villa Diaz13Joseph Criscione14Deok-Ho Kim15Todd J. Herron16Frankel Cardiovascular Regeneration Core Laboratory University of MichiganFrankel Cardiovascular Regeneration Core Laboratory University of MichiganKataoka CorporationKataoka CorporationKataoka CorporationFrankel Cardiovascular Regeneration Core Laboratory University of MichiganFrankel Cardiovascular Regeneration Core Laboratory University of MichiganFrankel Cardiovascular Regeneration Core Laboratory University of MichiganFrankel Cardiovascular Regeneration Core Laboratory University of MichiganFrankel Cardiovascular Regeneration Core Laboratory University of MichiganDepartment of Internal Medicine-Cardiology, University of MichiganDepartment of Internal Medicine-Cardiology, University of MichiganStemBioSys, Inc.Departments of Biological Sciences and Bioengineering, Oakland UniversityDepartment of Biomedical Engineering, Johns Hopkins UniversityDepartment of Biomedical Engineering, Johns Hopkins UniversityFrankel Cardiovascular Regeneration Core Laboratory University of MichiganAbstract Current methods for producing cardiomyocytes from human induced pluripotent stem cells (hiPSCs) using 2D monolayer differentiation are often hampered by batch-to-batch variability and inefficient purification processes. Here, we introduce CM-AI, a novel artificial intelligence-guided laser cell processing platform designed for rapid, label-free purification of hiPSC-derived cardiomyocytes (hiPSC-CMs). This approach significantly reduces processing time without the need for chronic metabolic selection or antibody-based sorting. By integrating real-time cellular morphology analysis and targeted laser ablation, CM-AI selectively removes non-cardiomyocyte populations with high precision. This streamlined process preserves cardiomyocyte viability and function, offering a scalable and efficient solution for cardiac regenerative medicine, disease modeling, and drug discovery.https://doi.org/10.1038/s42003-025-08162-0 |
| spellingShingle | Prakaimuk Saraithong Peyton Krajcarski Yukako Kusaka Moe Yamada Junichi Matsumoto Hailey Cunningham Sama Salih Darby Jones Devika Baddhan Christian Hausner Justus Anumonwo Anthony Rosenzweig Mary M. Navarro Luis Villa Diaz Joseph Criscione Deok-Ho Kim Todd J. Herron AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing Communications Biology |
| title | AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing |
| title_full | AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing |
| title_fullStr | AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing |
| title_full_unstemmed | AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing |
| title_short | AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing |
| title_sort | ai guided laser purification of human ipsc derived cardiomyocytes for next generation cardiac cell manufacturing |
| url | https://doi.org/10.1038/s42003-025-08162-0 |
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