Overexpression of syndecan-4 inhibits myogenesis by regulating the expression of myogenic regulatory factors
Syndecan-4, a type of heparin sulfate proteoglycan, plays an important role in muscle development, regeneration, and maintenance. Although, the important effects of syndecan-4 on the regulation of myogenesis in mice, turkeys, and bovines have been consistently reported, the molecular mechanisms of s...
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Korean Society of Animal Sciences and Technology
2025-03-01
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| Series: | Journal of Animal Science and Technology |
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| Online Access: | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e8 |
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| author | Sarang Choi Jeong-Woong Park Sang In Lee Sangsu Shin |
| author_facet | Sarang Choi Jeong-Woong Park Sang In Lee Sangsu Shin |
| author_sort | Sarang Choi |
| collection | DOAJ |
| description | Syndecan-4, a type of heparin sulfate proteoglycan, plays an important role in muscle development, regeneration, and maintenance. Although, the important effects of syndecan-4 on the regulation of myogenesis in mice, turkeys, and bovines have been consistently reported, the molecular mechanisms of syndecan-4 in myogenesis are not well understood. In this study, the role of syndecan-4 in regulating myogenesis was investigated in quail myoblas (QM7) cells, which constitute a quail myogenic cell line. Overexpression of syndecan-4 inhibited myogenesis, resulting in reduced myoblast fusion and shorter myotubes than in the control group. Therefore, the cells overexpressing syndecan-4 showed a smaller total myotube area than did the control cells. Furthermore, these cells had lesser myosin heavy chain proteins, suggesting that muscle differentiation is inhibited by syndecan-4. To investigate the inhibitory effect of syndecan-4 on myogenic differentiation, the mRNA expression levels in several genes known to regulate myoblast proliferation and differentiation were compared. Myogenic regulatory factors, including myogenic factor 5, myogenic differentiation 1, and myogenin, showed significantly different expressions between the groups during myogenesis. Myostatin, a negative regulator of muscle growth, showed significantly higher expression on day 4 in cells overexpressing syndecan-4. In conclusion, syndecan-4 could delay and inhibit muscle differentiation by regulating the expression levels of myogenic factors and muscle growth regulator in quail myocytes. This study provides valuable information regarding the role of syndecan-4 in myogenesis, which may aid in improving the production of poultry meat. |
| format | Article |
| id | doaj-art-5ef7eebe9dbe42f6975f0ed9f3348079 |
| institution | DOAJ |
| issn | 2672-0191 2055-0391 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj-art-5ef7eebe9dbe42f6975f0ed9f33480792025-08-20T03:18:37ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912025-03-0167241042010.5187/jast.2024.e8Overexpression of syndecan-4 inhibits myogenesis by regulating the expression of myogenic regulatory factorsSarang Choi0Jeong-Woong Park1Sang In Lee2Sangsu Shin3Department of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, KoreaCenter for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup 56212, KoreaDepartment of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, KoreaDepartment of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, KoreaSyndecan-4, a type of heparin sulfate proteoglycan, plays an important role in muscle development, regeneration, and maintenance. Although, the important effects of syndecan-4 on the regulation of myogenesis in mice, turkeys, and bovines have been consistently reported, the molecular mechanisms of syndecan-4 in myogenesis are not well understood. In this study, the role of syndecan-4 in regulating myogenesis was investigated in quail myoblas (QM7) cells, which constitute a quail myogenic cell line. Overexpression of syndecan-4 inhibited myogenesis, resulting in reduced myoblast fusion and shorter myotubes than in the control group. Therefore, the cells overexpressing syndecan-4 showed a smaller total myotube area than did the control cells. Furthermore, these cells had lesser myosin heavy chain proteins, suggesting that muscle differentiation is inhibited by syndecan-4. To investigate the inhibitory effect of syndecan-4 on myogenic differentiation, the mRNA expression levels in several genes known to regulate myoblast proliferation and differentiation were compared. Myogenic regulatory factors, including myogenic factor 5, myogenic differentiation 1, and myogenin, showed significantly different expressions between the groups during myogenesis. Myostatin, a negative regulator of muscle growth, showed significantly higher expression on day 4 in cells overexpressing syndecan-4. In conclusion, syndecan-4 could delay and inhibit muscle differentiation by regulating the expression levels of myogenic factors and muscle growth regulator in quail myocytes. This study provides valuable information regarding the role of syndecan-4 in myogenesis, which may aid in improving the production of poultry meat. http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e8syndecan-4QM7 cell lineMyogenic regulatory factorsMyogenesisMuscle differentiation |
| spellingShingle | Sarang Choi Jeong-Woong Park Sang In Lee Sangsu Shin Overexpression of syndecan-4 inhibits myogenesis by regulating the expression of myogenic regulatory factors Journal of Animal Science and Technology syndecan-4 QM7 cell line Myogenic regulatory factors Myogenesis Muscle differentiation |
| title | Overexpression of syndecan-4 inhibits myogenesis by regulating the expression of myogenic regulatory factors |
| title_full | Overexpression of syndecan-4 inhibits myogenesis by regulating the expression of myogenic regulatory factors |
| title_fullStr | Overexpression of syndecan-4 inhibits myogenesis by regulating the expression of myogenic regulatory factors |
| title_full_unstemmed | Overexpression of syndecan-4 inhibits myogenesis by regulating the expression of myogenic regulatory factors |
| title_short | Overexpression of syndecan-4 inhibits myogenesis by regulating the expression of myogenic regulatory factors |
| title_sort | overexpression of syndecan 4 inhibits myogenesis by regulating the expression of myogenic regulatory factors |
| topic | syndecan-4 QM7 cell line Myogenic regulatory factors Myogenesis Muscle differentiation |
| url | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e8 |
| work_keys_str_mv | AT sarangchoi overexpressionofsyndecan4inhibitsmyogenesisbyregulatingtheexpressionofmyogenicregulatoryfactors AT jeongwoongpark overexpressionofsyndecan4inhibitsmyogenesisbyregulatingtheexpressionofmyogenicregulatoryfactors AT sanginlee overexpressionofsyndecan4inhibitsmyogenesisbyregulatingtheexpressionofmyogenicregulatoryfactors AT sangsushin overexpressionofsyndecan4inhibitsmyogenesisbyregulatingtheexpressionofmyogenicregulatoryfactors |