Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
Abstract Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. Here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressi...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2016-08-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms12617 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850272859764555776 |
|---|---|
| author | Yi Zhang Zhen Liang Yuan Zong Yanpeng Wang Jinxing Liu Kunling Chen Jin-Long Qiu Caixia Gao |
| author_facet | Yi Zhang Zhen Liang Yuan Zong Yanpeng Wang Jinxing Liu Kunling Chen Jin-Long Qiu Caixia Gao |
| author_sort | Yi Zhang |
| collection | DOAJ |
| description | Abstract Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. Here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressing CRISPR/Cas9 introduced as DNA or RNA. This transient expression-based genome-editing system is highly efficient and specific for producing transgene-free and homozygous wheat mutants in the T0 generation. We demonstrate our protocol to edit genes in hexaploid bread wheat and tetraploid durum wheat, and show that we are able to generate mutants with no detectable transgenes. Our methods may be applicable to other plant species, thus offering the potential to accelerate basic and applied plant genome-engineering research. |
| format | Article |
| id | doaj-art-5eeff47d0d90427daddfe48074ff7432 |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2016-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-5eeff47d0d90427daddfe48074ff74322025-08-20T01:51:39ZengNature PortfolioNature Communications2041-17232016-08-01711810.1038/ncomms12617Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNAYi Zhang0Zhen Liang1Yuan Zong2Yanpeng Wang3Jinxing Liu4Kunling Chen5Jin-Long Qiu6Caixia Gao7State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesAbstract Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. Here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressing CRISPR/Cas9 introduced as DNA or RNA. This transient expression-based genome-editing system is highly efficient and specific for producing transgene-free and homozygous wheat mutants in the T0 generation. We demonstrate our protocol to edit genes in hexaploid bread wheat and tetraploid durum wheat, and show that we are able to generate mutants with no detectable transgenes. Our methods may be applicable to other plant species, thus offering the potential to accelerate basic and applied plant genome-engineering research.https://doi.org/10.1038/ncomms12617 |
| spellingShingle | Yi Zhang Zhen Liang Yuan Zong Yanpeng Wang Jinxing Liu Kunling Chen Jin-Long Qiu Caixia Gao Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA Nature Communications |
| title | Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA |
| title_full | Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA |
| title_fullStr | Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA |
| title_full_unstemmed | Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA |
| title_short | Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA |
| title_sort | efficient and transgene free genome editing in wheat through transient expression of crispr cas9 dna or rna |
| url | https://doi.org/10.1038/ncomms12617 |
| work_keys_str_mv | AT yizhang efficientandtransgenefreegenomeeditinginwheatthroughtransientexpressionofcrisprcas9dnaorrna AT zhenliang efficientandtransgenefreegenomeeditinginwheatthroughtransientexpressionofcrisprcas9dnaorrna AT yuanzong efficientandtransgenefreegenomeeditinginwheatthroughtransientexpressionofcrisprcas9dnaorrna AT yanpengwang efficientandtransgenefreegenomeeditinginwheatthroughtransientexpressionofcrisprcas9dnaorrna AT jinxingliu efficientandtransgenefreegenomeeditinginwheatthroughtransientexpressionofcrisprcas9dnaorrna AT kunlingchen efficientandtransgenefreegenomeeditinginwheatthroughtransientexpressionofcrisprcas9dnaorrna AT jinlongqiu efficientandtransgenefreegenomeeditinginwheatthroughtransientexpressionofcrisprcas9dnaorrna AT caixiagao efficientandtransgenefreegenomeeditinginwheatthroughtransientexpressionofcrisprcas9dnaorrna |