Optimization of Critical Factors for In Vitro Vegetative Growth of Pleurotus cornucopiae to Improve Branched Oyster Mushroom Production

The present investigation was carried out during (January–April) 2025 at Mushroom Research Laboratory, Department of Plant Pathology, College of Horticulture and Forestry, Neri, Hamirpur (HP) in which effect of different cultural parameters on vegetative growth of P. cornucopiae was evaluated. Mush...

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Main Authors: Arunesh Kumar, R. S. Jarial, Kumud Jarial, Savita Jandaik, Meenu Gupta, Chidembra Bhardwaj, Satish Kumar Sharma, Ankita, Surender Kumar
Format: Article
Language:English
Published: Puspa Publishing House 2025-07-01
Series:International Journal of Economic Plants
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Online Access:https://ojs.pphouse.org/index.php/IJEP/article/view/6609
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author Arunesh Kumar
R. S. Jarial
Kumud Jarial
Savita Jandaik
Meenu Gupta
Chidembra Bhardwaj
Satish Kumar Sharma
Ankita
Surender Kumar
author_facet Arunesh Kumar
R. S. Jarial
Kumud Jarial
Savita Jandaik
Meenu Gupta
Chidembra Bhardwaj
Satish Kumar Sharma
Ankita
Surender Kumar
author_sort Arunesh Kumar
collection DOAJ
description The present investigation was carried out during (January–April) 2025 at Mushroom Research Laboratory, Department of Plant Pathology, College of Horticulture and Forestry, Neri, Hamirpur (HP) in which effect of different cultural parameters on vegetative growth of P. cornucopiae was evaluated. Mushroom, an edible fungus, grows on moist surfaces such as wood or other organic matter including natural or semi-synthetic compost and consume globally due to their high nutritious vegetarian delicacy. The first crucial step in the successful generation of spawn was the preservation and restoration of a pure mycelium culture of exceptional quality. The fungus P. cornucopiae, branched oyster mushroom was widely used due to its nutritional and medicinal properties. Its cultivation was rather new in Indian conditions and much work has not yet been done. The solid and liquid media including potato dextrose, malt extract, oat meal, Asthana and Hawker’s and Czapek’s dox were evaluated in the present study for P. cornucopiae growth in which maximum diametric growth on potato dextrose agar and minimum on Asthana and Hawker’s agar were recorded. Similarly, maximum biomass was observed in potato dextrose broth and minimum in Asthana and Hawker’s broth. The growth was maximum under neutral and minimum under acidic condition, which ranged from sparse, fluffy to thick strand, and optimum at 25 °C and pH 7. It will require to validate these findings through comparative studies on different mushroom varieties.
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spelling doaj-art-5ed8e82aeef1424da0bd04f19bd9fa612025-08-20T03:16:15ZengPuspa Publishing HouseInternational Journal of Economic Plants2349-47352025-07-0112July, 410.23910/2/2025.6295bOptimization of Critical Factors for In Vitro Vegetative Growth of Pleurotus cornucopiae to Improve Branched Oyster Mushroom ProductionArunesh Kumar0R. S. Jarial1Kumud Jarial2Savita Jandaik3Meenu Gupta4Chidembra Bhardwaj5Satish Kumar Sharma6Ankita7Surender Kumar8Dept. of Plant Pathology, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh (173 230), IndiaDept. of Plant Pathology, College of Horticulture and Forestry, Neri, Hamirpur, Himachal Pradesh (177 001), IndiaDept. of Plant Pathology, College of Horticulture and Forestry, Neri, Hamirpur, Himachal Pradesh (177 001), IndiaDept. of Plant Pathology, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh (173 230), IndiaDept. of Vegetable Science, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh (173 230), IndiaDept. of Agriculture, Khalsa College Amritsar, Punjab (143 002), IndiaDept. of Plant Pathology, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh (173 230), IndiaDept. of Forestry, Post Graduate College of Agriculture, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur, Bihar (848 125), IndiaDept. of Biotechnology, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh (173 230), India The present investigation was carried out during (January–April) 2025 at Mushroom Research Laboratory, Department of Plant Pathology, College of Horticulture and Forestry, Neri, Hamirpur (HP) in which effect of different cultural parameters on vegetative growth of P. cornucopiae was evaluated. Mushroom, an edible fungus, grows on moist surfaces such as wood or other organic matter including natural or semi-synthetic compost and consume globally due to their high nutritious vegetarian delicacy. The first crucial step in the successful generation of spawn was the preservation and restoration of a pure mycelium culture of exceptional quality. The fungus P. cornucopiae, branched oyster mushroom was widely used due to its nutritional and medicinal properties. Its cultivation was rather new in Indian conditions and much work has not yet been done. The solid and liquid media including potato dextrose, malt extract, oat meal, Asthana and Hawker’s and Czapek’s dox were evaluated in the present study for P. cornucopiae growth in which maximum diametric growth on potato dextrose agar and minimum on Asthana and Hawker’s agar were recorded. Similarly, maximum biomass was observed in potato dextrose broth and minimum in Asthana and Hawker’s broth. The growth was maximum under neutral and minimum under acidic condition, which ranged from sparse, fluffy to thick strand, and optimum at 25 °C and pH 7. It will require to validate these findings through comparative studies on different mushroom varieties. https://ojs.pphouse.org/index.php/IJEP/article/view/6609Mushroom, Pleurotus cornucopiae, liquid media, temperature, pH
spellingShingle Arunesh Kumar
R. S. Jarial
Kumud Jarial
Savita Jandaik
Meenu Gupta
Chidembra Bhardwaj
Satish Kumar Sharma
Ankita
Surender Kumar
Optimization of Critical Factors for In Vitro Vegetative Growth of Pleurotus cornucopiae to Improve Branched Oyster Mushroom Production
International Journal of Economic Plants
Mushroom, Pleurotus cornucopiae, liquid media, temperature, pH
title Optimization of Critical Factors for In Vitro Vegetative Growth of Pleurotus cornucopiae to Improve Branched Oyster Mushroom Production
title_full Optimization of Critical Factors for In Vitro Vegetative Growth of Pleurotus cornucopiae to Improve Branched Oyster Mushroom Production
title_fullStr Optimization of Critical Factors for In Vitro Vegetative Growth of Pleurotus cornucopiae to Improve Branched Oyster Mushroom Production
title_full_unstemmed Optimization of Critical Factors for In Vitro Vegetative Growth of Pleurotus cornucopiae to Improve Branched Oyster Mushroom Production
title_short Optimization of Critical Factors for In Vitro Vegetative Growth of Pleurotus cornucopiae to Improve Branched Oyster Mushroom Production
title_sort optimization of critical factors for in vitro vegetative growth of pleurotus cornucopiae to improve branched oyster mushroom production
topic Mushroom, Pleurotus cornucopiae, liquid media, temperature, pH
url https://ojs.pphouse.org/index.php/IJEP/article/view/6609
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