Surface Display Technologies for Whole-Cell Biocatalysts: Advances in Optimization Strategies, Food Applications, and Future Perspectives
Surface display technology has revolutionized whole-cell biocatalysis by enabling efficient enzyme immobilization on microbial cell surfaces. Compared with traditional enzyme immobilization, this technology has the advantages of high enzyme activity, mild process, simple operation and low cost, whic...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/14/10/1803 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850257259263688704 |
|---|---|
| author | Baoyu Zhang Xing Gao Yu Zhou Shengping You Wei Qi Mengfan Wang |
| author_facet | Baoyu Zhang Xing Gao Yu Zhou Shengping You Wei Qi Mengfan Wang |
| author_sort | Baoyu Zhang |
| collection | DOAJ |
| description | Surface display technology has revolutionized whole-cell biocatalysis by enabling efficient enzyme immobilization on microbial cell surfaces. Compared with traditional enzyme immobilization, this technology has the advantages of high enzyme activity, mild process, simple operation and low cost, which thus has been widely studied and applied in various fields. This review explores the principles, optimization strategies, applications in the food industry, and future prospects. We summarize the membrane and anchor protein structures of common host cells (<i>Escherichia coli</i>, <i>Bacillus subtilis</i>, and yeast) and discuss cutting-edge optimization approaches, including host strain genetic engineering, rational design of anchor proteins, innovative linker peptide engineering, and precise regulation of signal peptides and promoters, to maximize surface display efficiency. Additionally, we also explore its diverse applications in food processing and manufacturing, additive synthesis, food safety, and other food-related industries (such as animal feed and PET packaging degradation), demonstrating their potential to address key challenges in the food industry. This work bridges fundamental research and industrial applications, offering valuable insights for advancing agricultural and food chemistry. |
| format | Article |
| id | doaj-art-5ed8a7db8321447db637063f2b70289e |
| institution | OA Journals |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-5ed8a7db8321447db637063f2b70289e2025-08-20T01:56:28ZengMDPI AGFoods2304-81582025-05-011410180310.3390/foods14101803Surface Display Technologies for Whole-Cell Biocatalysts: Advances in Optimization Strategies, Food Applications, and Future PerspectivesBaoyu Zhang0Xing Gao1Yu Zhou2Shengping You3Wei Qi4Mengfan Wang5School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaSchool of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaSchool of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, ChinaSurface display technology has revolutionized whole-cell biocatalysis by enabling efficient enzyme immobilization on microbial cell surfaces. Compared with traditional enzyme immobilization, this technology has the advantages of high enzyme activity, mild process, simple operation and low cost, which thus has been widely studied and applied in various fields. This review explores the principles, optimization strategies, applications in the food industry, and future prospects. We summarize the membrane and anchor protein structures of common host cells (<i>Escherichia coli</i>, <i>Bacillus subtilis</i>, and yeast) and discuss cutting-edge optimization approaches, including host strain genetic engineering, rational design of anchor proteins, innovative linker peptide engineering, and precise regulation of signal peptides and promoters, to maximize surface display efficiency. Additionally, we also explore its diverse applications in food processing and manufacturing, additive synthesis, food safety, and other food-related industries (such as animal feed and PET packaging degradation), demonstrating their potential to address key challenges in the food industry. This work bridges fundamental research and industrial applications, offering valuable insights for advancing agricultural and food chemistry.https://www.mdpi.com/2304-8158/14/10/1803whole-cell biocatalystsurface displayfood applicationsimmobilizationanchor proteinspassenger proteins |
| spellingShingle | Baoyu Zhang Xing Gao Yu Zhou Shengping You Wei Qi Mengfan Wang Surface Display Technologies for Whole-Cell Biocatalysts: Advances in Optimization Strategies, Food Applications, and Future Perspectives Foods whole-cell biocatalyst surface display food applications immobilization anchor proteins passenger proteins |
| title | Surface Display Technologies for Whole-Cell Biocatalysts: Advances in Optimization Strategies, Food Applications, and Future Perspectives |
| title_full | Surface Display Technologies for Whole-Cell Biocatalysts: Advances in Optimization Strategies, Food Applications, and Future Perspectives |
| title_fullStr | Surface Display Technologies for Whole-Cell Biocatalysts: Advances in Optimization Strategies, Food Applications, and Future Perspectives |
| title_full_unstemmed | Surface Display Technologies for Whole-Cell Biocatalysts: Advances in Optimization Strategies, Food Applications, and Future Perspectives |
| title_short | Surface Display Technologies for Whole-Cell Biocatalysts: Advances in Optimization Strategies, Food Applications, and Future Perspectives |
| title_sort | surface display technologies for whole cell biocatalysts advances in optimization strategies food applications and future perspectives |
| topic | whole-cell biocatalyst surface display food applications immobilization anchor proteins passenger proteins |
| url | https://www.mdpi.com/2304-8158/14/10/1803 |
| work_keys_str_mv | AT baoyuzhang surfacedisplaytechnologiesforwholecellbiocatalystsadvancesinoptimizationstrategiesfoodapplicationsandfutureperspectives AT xinggao surfacedisplaytechnologiesforwholecellbiocatalystsadvancesinoptimizationstrategiesfoodapplicationsandfutureperspectives AT yuzhou surfacedisplaytechnologiesforwholecellbiocatalystsadvancesinoptimizationstrategiesfoodapplicationsandfutureperspectives AT shengpingyou surfacedisplaytechnologiesforwholecellbiocatalystsadvancesinoptimizationstrategiesfoodapplicationsandfutureperspectives AT weiqi surfacedisplaytechnologiesforwholecellbiocatalystsadvancesinoptimizationstrategiesfoodapplicationsandfutureperspectives AT mengfanwang surfacedisplaytechnologiesforwholecellbiocatalystsadvancesinoptimizationstrategiesfoodapplicationsandfutureperspectives |