The Mechanosensitive PIEZO1 Channel Contributes to the Reaction of RAW264.7 Macrophages to Mechanical Strain
The mechanosensitive channel ‘piezo type mechanosensitive ion channel component 1’ (PIEZO1) plays a regulatory role in the response of periodontal ligament fibroblasts (PDLFs) to the mechanical strain that occurs during orthodontic tooth movement. In addition to PDLFs, immune cells such as macrophag...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/mi/9998838 |
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| author | Agnes Schröder Hanna Engelhardt Andressa Nogueira Björn Clausen Christian Kirschneck Jonathan Jantsch Peter Proff Kathrin Renner Eva Paddenberg-Schubert |
| author_facet | Agnes Schröder Hanna Engelhardt Andressa Nogueira Björn Clausen Christian Kirschneck Jonathan Jantsch Peter Proff Kathrin Renner Eva Paddenberg-Schubert |
| author_sort | Agnes Schröder |
| collection | DOAJ |
| description | The mechanosensitive channel ‘piezo type mechanosensitive ion channel component 1’ (PIEZO1) plays a regulatory role in the response of periodontal ligament fibroblasts (PDLFs) to the mechanical strain that occurs during orthodontic tooth movement. In addition to PDLFs, immune cells such as macrophages are also exposed to mechanical stimuli. Macrophages respond to mechanical strain with increased expression of inflammatory mediators. The role of PIEZO1 in this response remains elusive. To investigate the effect of PIEZO1 activation, RAW264.7 macrophages were stimulated with the PIEZO1 activator YODA1 without concurrent application of pressure. To further examine the specific role of PIEZO1 during mechanical strain, RAW264.7 macrophages were exposed to mechanical strain without and with simultaneous inhibition of PIEZO1 either by chemical inhibition (GsMTx4) or siRNA silencing. The expression of genes and proteins involved in orthodontic tooth movement was examined by quantitative PCR, western blot, and enzyme-linked immunosorbent assay (ELISA). Activation of PIEZO1 by YODA1 or mechanical strain increased the expression of inflammatory cytokines and osteoprotegerin (Opg), which is critically involved in bone remodeling processes. Conversely, inhibition of the PIEZO1 channel attenuates the effects of mechanical stress. In conclusion, our data demonstrate that the PIEZO1 channel is a major contributor to the response of macrophages to mechanical strain encountered during orthodontic tooth movement and affects the expression of inflammatory and bone remodeling factors. |
| format | Article |
| id | doaj-art-5ed3bbbc972c490181ebbc169af3a9e6 |
| institution | DOAJ |
| issn | 1466-1861 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5ed3bbbc972c490181ebbc169af3a9e62025-08-20T03:08:57ZengWileyMediators of Inflammation1466-18612025-01-01202510.1155/mi/9998838The Mechanosensitive PIEZO1 Channel Contributes to the Reaction of RAW264.7 Macrophages to Mechanical StrainAgnes Schröder0Hanna Engelhardt1Andressa Nogueira2Björn Clausen3Christian Kirschneck4Jonathan Jantsch5Peter Proff6Kathrin Renner7Eva Paddenberg-Schubert8Department of OrthodonticsDepartment of OrthodonticsDepartment of Periodontology and Operative DentistryInstitute for Molecular MedicineDepartment of OrthodonticsInstitute for Medical Microbiology and HygieneDepartment of OrthodonticsDepartment of OtorhinolaryngologyDepartment of OrthodonticsThe mechanosensitive channel ‘piezo type mechanosensitive ion channel component 1’ (PIEZO1) plays a regulatory role in the response of periodontal ligament fibroblasts (PDLFs) to the mechanical strain that occurs during orthodontic tooth movement. In addition to PDLFs, immune cells such as macrophages are also exposed to mechanical stimuli. Macrophages respond to mechanical strain with increased expression of inflammatory mediators. The role of PIEZO1 in this response remains elusive. To investigate the effect of PIEZO1 activation, RAW264.7 macrophages were stimulated with the PIEZO1 activator YODA1 without concurrent application of pressure. To further examine the specific role of PIEZO1 during mechanical strain, RAW264.7 macrophages were exposed to mechanical strain without and with simultaneous inhibition of PIEZO1 either by chemical inhibition (GsMTx4) or siRNA silencing. The expression of genes and proteins involved in orthodontic tooth movement was examined by quantitative PCR, western blot, and enzyme-linked immunosorbent assay (ELISA). Activation of PIEZO1 by YODA1 or mechanical strain increased the expression of inflammatory cytokines and osteoprotegerin (Opg), which is critically involved in bone remodeling processes. Conversely, inhibition of the PIEZO1 channel attenuates the effects of mechanical stress. In conclusion, our data demonstrate that the PIEZO1 channel is a major contributor to the response of macrophages to mechanical strain encountered during orthodontic tooth movement and affects the expression of inflammatory and bone remodeling factors.http://dx.doi.org/10.1155/mi/9998838 |
| spellingShingle | Agnes Schröder Hanna Engelhardt Andressa Nogueira Björn Clausen Christian Kirschneck Jonathan Jantsch Peter Proff Kathrin Renner Eva Paddenberg-Schubert The Mechanosensitive PIEZO1 Channel Contributes to the Reaction of RAW264.7 Macrophages to Mechanical Strain Mediators of Inflammation |
| title | The Mechanosensitive PIEZO1 Channel Contributes to the Reaction of RAW264.7 Macrophages to Mechanical Strain |
| title_full | The Mechanosensitive PIEZO1 Channel Contributes to the Reaction of RAW264.7 Macrophages to Mechanical Strain |
| title_fullStr | The Mechanosensitive PIEZO1 Channel Contributes to the Reaction of RAW264.7 Macrophages to Mechanical Strain |
| title_full_unstemmed | The Mechanosensitive PIEZO1 Channel Contributes to the Reaction of RAW264.7 Macrophages to Mechanical Strain |
| title_short | The Mechanosensitive PIEZO1 Channel Contributes to the Reaction of RAW264.7 Macrophages to Mechanical Strain |
| title_sort | mechanosensitive piezo1 channel contributes to the reaction of raw264 7 macrophages to mechanical strain |
| url | http://dx.doi.org/10.1155/mi/9998838 |
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